Increased susceptibility to metal catalysed oxidation of diabetic lens beta L crystallin: possible protection by dietary supplementation with acetylsalicylic acid.

abstract：PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...

journal_title：Experimental eye research

authors： Teixeira-Pinheiro LC,Toledo MF,Nascimento-Dos-Santos G,Mendez-Otero R,Mesentier-Louro LA,Santiago MF

更新日期：2020-11-01 00:00:00

abstract：:Polyclonal antisera have been made to synthetic peptides corresponding to the N- and C-terminal regions of the putative gamma 1-2 gene from human lens. These antisera are specific for gamma crystallin, showing no cross-reactivity with any polypeptides of the alpha- and beta-crystallin fractions. Western blot analysis ...

journal_title：Experimental eye research

authors： Takemoto L,Kodama T,Takemoto D

更新日期：1987-08-01 00:00:00

abstract：:Proliferative vitreoretinopathy (PVR) is a potentially blinding fibrotic complication. It is caused by the formation and contraction of epiretinal membranes (ERMs) that ultimately lead to retinal folds and traction retinal detachments. While multiple cell types have been identified in ERMs, retinal pigment epithelial ...

journal_title：Experimental eye research

authors： Tamiya S,Kaplan HJ

更新日期：2016-01-01 00:00:00

abstract：:Cathepsin D is a ubiquitous enzyme which plays an important role in the catabolism of proteins. Enzymatic studies showed that cathepsin D is the most important lysosomal enzyme in the proteolysis of opsin. The importance of cathepsin D in the lysosomal digestion of phagocytosed photoreceptor outer segments by the reti...

journal_title：Experimental eye research

authors： Rakoczy PE,Sarks SH,Daw N,Constable IJ

更新日期：1999-10-01 00:00:00

abstract：:Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is cap...

journal_title：Experimental eye research

authors： Meakin SO,Reddan JR,Tsui LC,Breitman ML

更新日期：1989-01-01 00:00:00

abstract：:Nerve growth factor in the tear film and corneal epithelium is hypothesized to play an important role in ocular surface maintenance and corneal wound healing. The purpose of this study was to determine the expression of nerve growth factor and its high affinity (trkA) receptor in tears, cornea, and lacrimal glands of ...

journal_title：Experimental eye research

authors： Woo HM,Bentley E,Campbell SF,Marfurt CF,Murphy CJ

更新日期：2005-05-01 00:00:00

abstract：:The aim of this study was to develop consistently focal elevated choroidal masses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A h...

journal_title：Experimental eye research

authors： Mueller AJ,Folberg R,Freeman WR,Bartsch DU,Bergeron-Lynn G,Mehaffey MG,Kan-Mitchell J,Huang X,Jian G,Avila C,Taskintuna I,Cheng L,Wang J

更新日期：1999-06-01 00:00:00

abstract：:The ocular hypotensive effect of topically applied prostaglandins (PGs) is well documented. Although PGs introduced in the posterior chamber accumulate in the anterior tissues (e.g. iris/ciliary complex), little is known about the metabolism of PGs by these tissues. We have recently found that non-pigmented epithelial...

journal_title：Experimental eye research

authors： Asakura T,Shichi H

更新日期：1992-08-01 00:00:00

abstract：:A key model for examining the activity-dependent development of primary visual cortex (V1) involves the imbalance in activity between the two eyes induced by monocular deprivation (MD). MD early in life causes dramatic changes in the functional and structural organization of mammalian visual cortex. The molecular sign...

journal_title：Experimental eye research

authors： Shan L,Yong H,Song Q,Wei Y,Qin R,Zhang G,Xu M,Zhang S

更新日期：2013-04-01 00:00:00

abstract：:The scleral spur in 37 human (age 17-87 years) and six cynomolgus monkey eyes (2-4 years) was investigated. Serial meridional and tangential sections were studied with ultrastructural and immunocytochemical methods. The bundles of the ciliary muscle do not enter the scleral spur, but their tendons, which consist of el...

journal_title：Experimental eye research

authors： Tamm E,Flügel C,Stefani FH,Rohen JW

更新日期：1992-04-01 00:00:00

abstract：:The aim of the study was to determine anatomical and growth factor profiles in patients with clinically significant macular oedema (CSMO) undergoing pars plana vitrectomy (PPV). Twenty patients with moderate nonproliferative diabetic retinopathy (NPDR) with persistent CSMO underwent PPV. Patients had baseline and post...

journal_title：Experimental eye research

authors： Patel JI,Tombran-Tink J,Hykin PG,Gregor ZJ,Cree IA

更新日期：2006-05-01 00:00:00

abstract：:Beige adipocytes and brown adipocytes can generate heat by using mitochondrial uncoupling protein 1 (Ucp1), a thermogenic protein. Browning/beiging is the emergence of beige adipocytes in white adipose tissues (WAT) for cold acclimatization. Here we show the existence of brown/beige adipocytes in retro-orbital WAT in ...

journal_title：Experimental eye research

authors： Sugiyama M,Shindo D,Kanada N,Ohzeki T,Yoshioka K,Funaba M,Hashimoto O

更新日期：2019-07-01 00:00:00

abstract：:Selective pericyte loss, the histological hallmark of early diabetic retinopathy (DR), enhances the breakdown of the blood-retinal barrier (BRB) in diabetes. However, the role of pericytes on BRB alteration in diabetes and the signaling pathways involved in their effects are currently unknown. To understand the role o...

journal_title：Experimental eye research

authors： Rangasamy S,Monickaraj F,Legendre C,Cabrera AP,Llaci L,Bilagody C,McGuire P,Das A

更新日期：2020-06-01 00:00:00

abstract：:To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...

journal_title：Experimental eye research

authors： Midelfart A,Dybdahl A,MUller N,Sitter B,Gribbestad IS,Krane J

更新日期：1998-03-01 00:00:00

abstract：:We have previously shown that corticotropin-releasing hormone plays an important proinflammatory role in the induction of experimental autoimmune uveoretinitis. In this study, we examined the role of apoptosis in the destruction of the retina during experimental autoimmune uveoretinitis, and the role of corticotropin-...

journal_title：Experimental eye research

authors： Poulaki V,Mitsiades N,Mastorakos G,Caspi RR,Chrousos GP,Bouzas E

更新日期：2001-06-01 00:00:00

abstract：:Rod outer segments (ROS) isolated from adult rat retinas are phagocytized by cultured rat retinal pigment epithelial (RPE) cells. Using a double immunofluorescent labeling procedure, we have compared the binding and ingestion of ROS isolated at different times of the day. After 2 hr of incubation, approximately 98% of...

journal_title：Experimental eye research

authors： Colley NJ,Hall MO

更新日期：1986-04-01 00:00:00

abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...

journal_title：Experimental eye research

authors： Smith WC

更新日期：1996-06-01 00:00:00

abstract：:Previous studies have suggested that a mannose receptor mediates the phagocytic uptake of effete rod outer segments by retinal pigment epithelial cells. In the present study, the effect of adding a soluble ligand for the mannose receptor, horseradish peroxidase, was examined. Cultured retinal pigment epithelial cells ...

journal_title：Experimental eye research

authors： Lutz DA,Guo Y,McLaughlin BJ

更新日期：1995-10-01 00:00:00

abstract：:We studied a new fluorophotometric method to determine trabecular outflow facility in man. Pilocarpine was selected to evaluate this non-invasive method, since its primary ocular hypotensive effect is to increase trabecular outflow facility. Levobunolol was used to decrease intraocular pressure and aqueous humor flow ...

journal_title：Experimental eye research

authors： Hayashi M,Yablonski ME,Novack GD

更新日期：1989-05-01 00:00:00

abstract：:Congenic hereditary cataract mice, BALB/c-nct/nct, were established by introducing the nct gene from Nakano into BALB/c mice. These mice developed a milder cortical form of cataract which developed sporadically and later in life than in Nakano mice. Combined use of BALB/c and BALB/c-nct/nct mice enables biochemical co...

journal_title：Experimental eye research

authors： Wada E,Koyama-Ito H,Matsuzawa A

更新日期：1991-05-01 00:00:00

abstract：:Vision loss due to various forms of outer retinal degeneration remains a major problem in clinical ophthalmology. Most retinal degenerations are precipitated by genetic mutations affecting the retinal pigment epithelium and sensory retina, but it is becoming increasingly evident that resultant metabolic changes within...

journal_title：Experimental eye research

authors： Yu DY,Cringle SJ

更新日期：2005-06-01 00:00:00

abstract：:Recently, a unique distribution, namely a reduction of macular pigment optical density (MPOD) within the central retina with a surrounding ring-like structure of preserved MPOD at about 6 degrees eccentricity was suggested to be a common finding in macular telangiectasia (MacTel) type 2. In order to quantify this redu...

journal_title：Experimental eye research

authors： Charbel Issa P,van der Veen RL,Stijfs A,Holz FG,Scholl HP,Berendschot TT

更新日期：2009-06-15 00:00:00

abstract：:Intravitreal cidofovir has been shown to be a long acting and highly efficacious treatment for CMV retinitis; however decrease in IOP is an adverse effect. We wanted to determine the effect of cidofovir on intraocular pressure (IOP) in the guinea pig, and rabbit eye to develop an animal model of cidofovir induced ocul...

journal_title：Experimental eye research

authors： Taskintuna I,Banker AS,Rao NA,Wiley CA,Flores-Aguilar M,Munguia D,Bergeron-Lynn G,De Clercq E,Keefe K,Freeman WR

更新日期：1997-05-01 00:00:00

abstract：:Limbal microvascular endothelial cells (L-MVEC) contribute to formation of the corneal-limbal stem cell niche and to neovascularization of diseased and injuries corneas. Nevertheless, despite these important roles in corneal health and disease, few attempts have been made to isolate L-MVEC with the view to studying th...

journal_title：Experimental eye research

authors： Gillies PJ,Bray LJ,Richardson NA,Chirila TV,Harkin DG

更新日期：2015-02-01 00:00:00

abstract：:Rho GTPase regulated contractile signaling in the trabecular meshwork (TM) has been shown to modulate aqueous humor (AH) outflow and intraocular pressure (IOP). To explore whether elevated IOP, a major risk factor for primary open angle glaucoma (POAG) influences Rho GTPase signaling in the TM, we recorded AH outflow ...

journal_title：Experimental eye research

authors： Pattabiraman PP,Inoue T,Rao PV

更新日期：2015-07-01 00:00:00

abstract：:For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...

journal_title：Experimental eye research

authors： Zhang P,Mocci J,Wahl DJ,Meleppat RK,Manna SK,Quintavalla M,Muradore R,Sarunic MV,Bonora S,Pugh EN Jr,Zawadzki RJ

更新日期：2018-07-01 00:00:00

abstract：:Fungal keratitis is a common disease that causes blindness. An effective animal model for fungal keratitis is essential for advancing research on this disease. Our objective is to develop a novel mouse model of Fusarium solani keratitis through the inoculation of fluorescent-labeled fungi into the cornea to facilitate...

journal_title：Experimental eye research

authors： Zhang H,Wang L,Li Z,Liu S,Xie Y,He S,Deng X,Yang B,Liu H,Chen G,Zhao H,Zhang J

更新日期：2013-05-01 00:00:00

abstract：:Primary cultures of chick lens annular pad (CLAP) cells were exposed to beta-adrenergic drugs and lipid soluble cAMP analogues. In dose-dependent manners, these agents inhibited re-entry into the cell cycle when these normally post-mitotic cells were cultured in the presence of serum. The inhibition of cell division w...

journal_title：Experimental eye research

authors： Ireland ME,Tran K,Mrock L

更新日期：1993-09-01 00:00:00

abstract：:Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...

journal_title：Experimental eye research

authors： Richardson R,Smart M,Tracey-White D,Webster AR,Moosajee M

更新日期：2017-02-01 00:00:00

abstract：:As wounds in stratified epithelia close, the numbers of cells at the leading edge of migration decreases. It is not known whether cells at the leading edge are continually replaced or whether some retain their position at the leading edge over time. Replication-deficient adenovirus carrying the green fluorescent prote...

journal_title：Experimental eye research

authors： Danjo Y,Gipson IK

更新日期：2002-02-01 00:00:00