Abstract：
:The aim of the study was to determine anatomical and growth factor profiles in patients with clinically significant macular oedema (CSMO) undergoing pars plana vitrectomy (PPV). Twenty patients with moderate nonproliferative diabetic retinopathy (NPDR) with persistent CSMO underwent PPV. Patients had baseline and postoperative clinical assessment including Ocular Coherence Tomography (OCT). Baseline vitreous and aqueous and serial postoperative aqueous samples were analysed for vascular endothelial growth factor-A (VEGF-A), pigment epithelium derived Factor (PEDF) and other factors (pg/ml) including hepatocyte growth factor, MMP 9, soluble flt-1 Receptor, and TGF beta1 by ELISA. Vitreous from patients with full thickness macular holes (8) and proliferative diabetic retinopathy (22) were collected for comparison as controls. Vitreous VEGF-A concentration in the NPDR group was 957 pg/ml compared to 239 pg/ml in the macula hole (FTMH) control (p < 0.0001) and 596 pg/ml compared to PDR (p = 0.006). The median diabetic vitreous PEDF concentration was 1.36 microg/ml (FTMH 2.6 microg/ml p = 0.05). In NPDR, it was higher (1.59 microg/ml) than PDR (1.27 microg/ml) p = 0.02. There were changes to the HGF, soluble flt-1 Receptor and TGF b1 concentrations in the NPDR compared to either PDR or the normal state. In CSMO, two OCT profiles were identified: dome-shaped macular elevation (Group 1) (n = 4) and diffuse-low elevation profile (Group 2) (n = 16) which also showed differences in the postoperative median aqueous VEGF concentrations despite macular volume decreasing for both. The results suggest that there is an up-regulation of VEGF in the vitreous of the diabetic eye with a reciprocal decrease in PEDF. The structural and molecular differences between the two OCT macular profiles may explain the varying response to PPV in patients with diffuse CSMO.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Patel JI,Tombran-Tink J,Hykin PG,Gregor ZJ,Cree IAdoi
10.1016/j.exer.2005.10.002keywords:
subject
Has Abstractpub_date
2006-05-01 00:00:00pages
798-806issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(05)00324-6journal_volume
82pub_type
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：2017-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.015
更新日期：2018-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0422
更新日期：1998-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90004-e
更新日期：1990-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107700
更新日期：2019-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.015
更新日期：2011-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.010
更新日期：2017-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1146
更新日期：1993-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.07.009
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0079
更新日期：1996-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0133
更新日期：1996-10-01 00:00:00
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更新日期：2018-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1988-06-01 00:00:00
abstract：:We examine the immunoreactivity of the non-receptor tyrosine kinase, c-Src kinase and its downstream molecule, signal transducer and activator of transcription 3 (pStat3) in retinoblastoma (RB), and correlation with invasiveness and differentiation. Tumor samples from 40 patients with RB were available for the study. ...
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doi：10.1016/j.exer.2006.02.011
更新日期：2006-10-01 00:00:00
