Abstract:
:Electrical stimulation of the preganglionic cervical sympathetic nerve produced ocular vasoconstrictor responses as measured from the anterior choroid of anesthetized cats using laser-Doppler flowmetry. Ipsilateral nictitating membrane contractions were simultaneously measured as an established index of neural sympathetic activation. The frequency-response relationships for both effectors were nearly linear from 0.125-32 Hz. When elicited at 2-min intervals, submaximal evoked responses of both systems were stable for more than 1 hr. Ocular vasoconstrictor and nictitating membrane responses were blocked in a dose-dependent fashion by intravenous treatment with the non-selective alpha-adrenoceptor antagonist phentolamine (0.1-3.0 mg kg-1) and with phenoxybenzamine (3.0 mg kg-1) as well as with the selective alpha 1-adrenoceptor antagonist, prazosin (10-300 micrograms kg-1). In contrast, neither evoked response was antagonized by alpha 2-adrenoceptor blocker, yohimbine (10-300 micrograms kg-1), nor with rauwolscine (500 micrograms kg-1). No significant alteration in magnitude of ocular vasoconstriction was seen with blockade of either muscarinic (atropine, 1 mg kg-1) or beta-adrenergic (propranolol, 1 mg kg-1) receptors. These results demonstrate the usefulness of laser-Doppler flowmetry in studies of the choroidal circulation and suggest that, as previously established for the nictitating membrane, adrenergic neurogenic vasoconstriction in the anterior segment of the eye is mediated almost exclusively by alpha 1-adrenoceptor mechanisms.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Koss MC,Gherezghiher Tdoi
10.1006/exer.1993.1146subject
Has Abstractpub_date
1993-10-01 00:00:00pages
441-7issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71146-2journal_volume
57pub_type
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