Abstract：
:Several studies have suggested that antioxidants retard the process of cataractogenesis by scavenging free oxygen radicals. The present study sought to assess the efficacy of the antioxidant acetyl-L-carnitine (ALCAR) in preventing selenite-induced cataractogenesis in an experimental setting. The first, in vitro phase of the study was performed on lenses from Wistar rats incubated for 24 h at 37 degrees C in Dulbecco's Modified Eagle Medium (DMEM) alone (control, Group I), or in DMEM containing 100 microM of selenite (Group II) or in DMEM containing 100 microM of selenite and 200 microM/ml ALCAR added at the same time as selenite (Group IIIa) or 30 min, 1 h or 2 h later (Groups IIIb, IIIc and IIId, respectively). Gross morphological examination of these lenses revealed dense opacification (cataract formation) in Group II, minimal opacification in some Group IIIa lenses and no opacification in Group I. The mean activities of the antioxidant enzymes catalase and glutathione peroxidase were significantly lower in Group II than in Group I or Group IIIa lenses, while malondialdehyde concentration (an indicator of lipid peroxidation) was significantly higher in Group II lenses than that in Group I or Group IIIa lenses. The second, in vivo phase of the study revealed dense opacification (cataract formation) in 100% of Wistar rat pups receiving subcutaneous sodium selenite alone (19 microM/kg body weight) but in only 37.5% of those receiving subcutaneous selenite and intraperitoneal ALCAR. These data suggest that ALCAR is able to significantly retard experimental selenite-induced cataractogenesis.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Geraldine P,Sneha BB,Elanchezhian R,Ramesh E,Kalavathy CM,Kaliamurthy J,Thomas PAdoi
10.1016/j.exer.2006.07.009subject
Has Abstractpub_date
2006-12-01 00:00:00pages
1340-9issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(06)00310-1journal_volume
83pub_type
杂志文章abstract：:Interphotoreceptor retinoid-binding protein (IRBP) greatly enhances the conversion of all- trans -retinol to 11- cis -retinal by the retinal pigment epithelium (RPE) and facilitates 11- cis -retinal release from the RPE. However, the mechanisms by which IRBP exerts these effects are not clear. Using a model system of ...
journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：1988-11-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1186
更新日期：1993-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90051-f
更新日期：1991-05-01 00:00:00
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更新日期：2018-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1993-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1057
更新日期：1994-05-01 00:00:00
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更新日期：1989-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.02.005
更新日期：2007-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1997-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1983-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90045-9
更新日期：1984-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90008-g
更新日期：1992-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80051-x
更新日期：1995-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
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更新日期：2011-08-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2015-12-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2003.08.006
更新日期：2004-03-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2003-09-01 00:00:00
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更新日期：2018-03-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1997.0336
更新日期：1997-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1986-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107826
更新日期：2019-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.09.017
更新日期：2010-01-01 00:00:00
abstract：:The purpose of this study was to evaluate specific keratocyte phenotypes (keratocyte, fibroblast, myofibroblast) for cell contractility and ability to contract extracellular matrix. Rabbit keratocyte phenotype was modulated by exposure to optimal proliferative doses of IGF-I, IL-1alpha, FGF2, PDGF-AB, and TGFbeta(1). ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00188-x
更新日期：2003-11-01 00:00:00
