Abstract：
:Longitudinal (T1) magnetic relaxation times for the major phosphorus-containing metabolites present in the bovine and rabbit lens under organ culture conditions and in the bovine and rabbit globe have been determined. Significant differences in T1 for the major phosphorus metabolites in each case are observed, as well as for the same metabolite in the two species examined. Species-dependent lens hydration may account, in part, for these differences. Because of the requirement for rapid repetitive pulsing for the attainment of optimum signal collection efficiency by the Fourier transform nuclear magnetic resonance method, significant differential saturation of metabolite resonance intensities occurs in circumstances where appreciable differences in T1 relaxation times are present, which, unless corrected, leads to erroneous determinations of relative metabolite levels. The net effect of assessing relative metabolite levels in terms of the percentage of total phosphate signal, without a correction for T1 discrimination, is to underestimate metabolites with a long T1 (sugar phosphates) and overestimate those metabolites with a short T1 (ATP). Individual metabolite T1 discrimination factors are calculated from integrated areas of spectra acquired using short and long repetition times as well as from metabolite T1 values. They are then employed, for the first time, for the correction of 31P-NMR spectra of bovine and rabbit lenses. Corrected spectra provide relative metabolite levels for lenticular ATP which are in excellent agreement with values determined by chemical and enzymatic assays.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Schleich T,Willis JA,Matson GBdoi
10.1016/0014-4835(84)90045-9subject
Has Abstractpub_date
1984-10-01 00:00:00pages
455-68issue
4eissn
0014-4835issn
1096-0007pii
0014-4835(84)90045-9journal_volume
39pub_type
杂志文章abstract：:Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In th...
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：1990-06-01 00:00:00
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更新日期：1992-10-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1987-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2016-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.11.008
更新日期：2007-03-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1121
更新日期：2002-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90164-n
更新日期：1992-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90089-2
更新日期：1985-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4835(91)90051-f
更新日期：1991-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
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更新日期：1990-11-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0250
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2005.06.020
更新日期：2006-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0599
更新日期：1999-02-01 00:00:00
abstract：:Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.01.001
更新日期：2017-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00175-1
更新日期：2003-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.02.012
更新日期：2017-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.11.002
更新日期：2005-04-01 00:00:00
abstract：:Identification of causal genetic defects for human diseases took a significant leap when the first generation DNA sequencing technologies enabled biologists extract sequence-based genetic information from living beings. However, these sequencing methods had unavoidable constraints of throughput, scalability, rapidity,...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.08.013
更新日期：2017-11-01 00:00:00
