Abstract:
:We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolonged periods of anoxia, we examined whether anoxia could induce retinal damage. Three hours of anoxia induced in the retina the development of apoptotic cell death, as assessed 48 h later by TUNEL staining. TUNEL-positive cells were particularly found in the inner and outer nuclear layers, and were also present in the ganglion cell layer. Pharmacological activation of mGlu2/3 receptors by systemic injection of LY379268 (0.5 mg/kg, i.p., 15 min before the onset of anoxia) substantially protected retinas against anoxia-induced cell death. In contrast, systemic injection of the mGlu2/3 receptor antagonist, LY341495 (1 mg/kg, i.p., 15 min before the onset of anoxia), significantly amplified cell death. Finally, as mGlu2/3 receptors are implicated in the control of extracellular glutamate concentrations, we examined the stimulation of glutamate release in isolated goldfish retinas. Depolarizing medium containing 30 mM KCl led to a significant increase in glutamate release, which was substantially reduced by LY379268. We conclude that activation of mGlu2/3 receptors may provide a major defensive mechanism against ischemic/anoxic retinal damage.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Beraudi A,Bruno V,Battaglia G,Biagioni F,Rampello L,Nicoletti F,Poli Adoi
10.1016/j.exer.2006.11.008subject
Has Abstractpub_date
2007-03-01 00:00:00pages
544-52issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(06)00449-0journal_volume
84pub_type
杂志文章abstract::A number of variants of the wild-type (wt) estrogen receptor beta (ERbeta-1) coexist in a wide range of tissues. In the human these include, together with others, the expression of several isoforms (ERbeta-2-ERbeta-5) due to alternative splicing of exons encoding the carboxy terminus. In this study, we determined whet...
journal_title:Experimental eye research
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abstract::Electrical field stimulation produced contractions of the rabbit isolated iris sphincter muscle which were completely blocked by tetrodotoxin (10(-6) M). Low frequency (0.16-5 Hz) responses were atropine-resistant, but were prevented by sensory (trigeminal) denervation, and were considered to be owing to antidromic st...
journal_title:Experimental eye research
pub_type: 杂志文章
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abstract::This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title:Experimental eye research
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abstract::A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hat...
journal_title:Experimental eye research
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journal_title:Experimental eye research
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abstract::Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the con...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.11.019
更新日期:2017-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.008
更新日期:2007-04-01 00:00:00
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journal_title:Experimental eye research
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pub_type: 杂志文章
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更新日期:2019-11-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(88)90037-1
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.01.004
更新日期:2005-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.09.011
更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2013.12.002
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pub_type: 杂志文章
doi:10.1016/j.exer.2016.10.001
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abstract::The mass receptor potential of the excised, superfused retina of the bullfrog was studied. Photoreceptor responses were isolated by addition of sodium aspartate to the Ringer solutions. Responses of the cones were monitored independently from responses of the rods by employing a two-flash method of stimulation which t...
journal_title:Experimental eye research
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doi:10.1016/0014-4835(84)90007-1
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更新日期:2013-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0883
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abstract::Neuroretinal diseases are the predominant cause of irreversible blindness worldwide, mainly due to photoreceptor loss. Currently, there are no radical treatments to fully reverse the degeneration or even stop the disease progression. Thus, it is urgent to develop new biological therapeutics for these diseases on the c...
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0087
更新日期:1996-07-01 00:00:00
abstract::The purpose of this study was to investigate the influence of molecular shape, conformability, net surface charge and tissue interaction on transscleral diffusion. Unfixed, porcine sclera was clamped in an Ussing chamber. Fluorophore-labelled neutral albumin, neutral dextran, or neutral ficoll were placed in one hemi-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.07.007
更新日期:2012-09-01 00:00:00
abstract::The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
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