Abstract：
:Previously, we demonstrated that the inwardly rectifying K(+) (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that in addition to Kir7.1, seven other Kir channel subunits (Kir1.1, Kir2.1, Kir2.2, Kir3.1, Kir3.4, Kir4.2 and Kir6.1) are expressed in the RPE, whereas in neural retina, all 14 of the Kir channel subunits examined are expressed. The identities of RT-PCR products in the RPE were confirmed by DNA sequencing. Real-time RT-PCR analysis showed, however, that transcripts of these channels are significantly less abundant than Kir7.1 in the RPE. Western blot analysis of the Kir channel subunits detected in the RPE by RT-PCR revealed the expression of Kir2.1, Kir3.1, Kir3.4, Kir4.2, Kir6.1, and possibly Kir2.2, but not Kir1.1, in both human RPE and neural retina. Our results indicate that human RPE expresses at least five other Kir channel subtypes in addition to Kir7.1, suggesting that multiple members of the Kir channel family may function in this epithelium.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Yang D,Zhang X,Hughes BAdoi
10.1016/j.exer.2008.05.010subject
Has Abstractpub_date
2008-09-01 00:00:00pages
176-83issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(08)00156-5journal_volume
87pub_type
杂志文章abstract：:A potential role for myosin light chain kinase (MLCK) in regulating intraocular pressure and outflow function has recently been reported in living monkey eye and rabbit eye. There is little information about the effects of the ocular hypotensive agents, prostaglandin F2alpha (PGF2alpha) and latanoprost on this signali...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.10.015
更新日期：2004-02-01 00:00:00
abstract：:Longitudinal (T1) magnetic relaxation times for the major phosphorus-containing metabolites present in the bovine and rabbit lens under organ culture conditions and in the bovine and rabbit globe have been determined. Significant differences in T1 for the major phosphorus metabolites in each case are observed, as well...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90045-9
更新日期：1984-10-01 00:00:00
abstract：:The mass receptor potential of the excised, superfused retina of the bullfrog was studied. Photoreceptor responses were isolated by addition of sodium aspartate to the Ringer solutions. Responses of the cones were monitored independently from responses of the rods by employing a two-flash method of stimulation which t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90007-1
更新日期：1984-08-01 00:00:00
abstract：:The polypeptides in portions of human lens epithelium from individuals of various ages were resolved by two-dimensional (2D) gel electrophoresis. The epithelium was divided into a central area of 12.5 mm2, a surrounding area of 50.2 mm2, and an area which was outside of this region. The proteins were solubilized in ur...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90064-l
更新日期：1991-05-01 00:00:00
abstract：:The ocular lens fiber cell assembles a novel cytoskeletal element, the Beaded Filament, from CP49 and filensin, two proteins expressed only in the differentiated lens fiber cell. We report the primary sequence, secondary structural analysis, gene structure and Yeast Two Hybrid interaction data for human filensin, and ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0478
更新日期：1998-05-01 00:00:00
abstract：:Axial-length and refraction measurements are presented for the lid-sutured rhesus monkey at 6-month intervals during the first 1.5 years of life. The experimental data are fitted to an exponential growth theory allowing the calculation of time constants, apparent time origins, and correlation coefficients for 10 eyes ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90043-6
更新日期：1986-06-01 00:00:00
abstract：:The purpose of this study was to investigate the effects of abnormal-cannabidiol (abn-cbd), a non-psychoactive cannabinoid agonist, on aqueous humor outflow via the trabecular meshwork (TM) of porcine eye, and to examine the involvement of a non-CB1/CB2 cannabinoid receptor and the p42/44 mitogen-activated protein kin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.05.001
更新日期：2012-07-01 00:00:00
abstract：:It has been suggested that ambient lighting at night influences eye growth and might play a causal role in human myopia. To test this hypothesis, we reared newly hatched chicks under 12 hr light-dark or light-dim cycles with a light phase intensity of 1500 microW/cm(2) and variable dim phase intensities between 0.01 a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.08.007
更新日期：2004-11-01 00:00:00
abstract：:Lacritin is a mitogen of human salivary gland cells as well as a stimulator of human corneal epithelial cells. It is expected to be an important factor in maintaining the surrounding ocular surface. The monkey would be a relevant animal model in which to study the role of lacritin in ophthalmic physiology and patholog...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.019
更新日期：2007-11-01 00:00:00
abstract：:The current study investigates potential differences in fibroblast phenotype across the anterior segment of the human eye with the aim to understanding factors that support the regenerative function of human limbal epithelial progenitor cells (LEPs) during wound healing. Separate cultures of fibroblasts were establish...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.10.004
更新日期：2011-01-01 00:00:00
abstract：:The in vitro and in vivo effects of a new immunomodulating agent, bucillamine, on experimental autoimmune uveitis (EAU) was studied in the rat. The capacity of S-antigen-sensitized lymphocytes to proliferate in response to the antigen or to produce antigen-specific antibodies was significantly suppressed by bucillamin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1036
更新日期：1994-04-01 00:00:00
abstract：:Selective pericyte loss, the histological hallmark of early diabetic retinopathy (DR), enhances the breakdown of the blood-retinal barrier (BRB) in diabetes. However, the role of pericytes on BRB alteration in diabetes and the signaling pathways involved in their effects are currently unknown. To understand the role o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108043
更新日期：2020-06-01 00:00:00
abstract：:As wounds in stratified epithelia close, the numbers of cells at the leading edge of migration decreases. It is not known whether cells at the leading edge are continually replaced or whether some retain their position at the leading edge over time. Replication-deficient adenovirus carrying the green fluorescent prote...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1115
更新日期：2002-02-01 00:00:00
abstract：:Post-translational modifications by transglutaminase may contribute to the remodeling of cellular architecture in the development of lens fiber cells, and there is evidence that the enzyme may also play a role in cataract formation. It catalyses hydrolytic deamidations as well as amide exchanges on select glutamine si...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0463
更新日期：1998-05-01 00:00:00
abstract：:Choroidal neovascularization (CNV) associated with the 'wet' form of age related macular degeneration (AMD) is one of the most common causes of central vision loss among the elderly. The 'wet' form of AMD is currently treated by intravitreal delivery of anti-VEGF agents. However, intravitreal injections are associated...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.09.005
更新日期：2015-11-01 00:00:00
abstract：:Several scleral cross-linking (SXL) methods were suggested to increase the biomechanical stiffness of scleral tissue and therefore, to inhibit axial eye elongation in progressive myopia. In addition to scleral cross-linking and biomechanical effects caused by riboflavin and light irradiation such a treatment might ind...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.07.005
更新日期：2015-10-01 00:00:00
abstract：:Retinal degeneration (RD) results from photoreceptor apoptosis. Cell transplantation, one potential therapeutic approach, requires expandable stem cells that can form mature photoreceptors when differentiated. Freshly dissociated primary retinal cells from postnatal day 2-6 (PN2-6) mouse retina can give rise, post-tra...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90095-v
更新日期：1991-03-01 00:00:00
abstract：:Mutations in the optic atrophy type 1 (OPA1) gene give rise to human autosomal dominant optic atrophy. The purpose of this study is to investigate OPA1 protein expression in the human retina and optic nerve. A rabbit polyclonal antiserum was generated using a fusion protein covering amino acids 647 to 808 of the human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.06.004
更新日期：2006-11-01 00:00:00
abstract：:The causes of vitreous humor (VH) liquefaction remain unclear. Diabetes accelerates this process and other ocular diseases. The weakening of the blood-retina barrier observed with diabetes could enhance the rate of transfer of relatively small molecules such as glucose (Glu) and phospholipids (PLs) from the retina to ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108221
更新日期：2020-11-01 00:00:00
abstract：:The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107869
更新日期：2020-01-01 00:00:00
abstract：:Accurate thermal models for the cornea of the eye support the development of thermal techniques for reshaping the cornea and other scientific purposes. Heat transfer in the cornea must be quantified accurately so that a thermal treatment does not destroy the endothelial layer, which cannot regenerate, and yet is respo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.029
更新日期：2013-10-01 00:00:00
abstract：:Retinal stimulation with high spatial resolution requires close proximity of electrodes to target cells. This study examines the effects of material coatings and 3-dimensional geometries of subretinal prostheses on their integration with the retina. A trans-scleral implantation technique was developed to place microfa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.09.018
更新日期：2009-01-01 00:00:00
abstract：:Lens lipid composition and lipid hydrocarbon chain structure change with age, region and cataract. Since the lens Ca(2+)-ATPase pump is important to the maintenance of calcium homeostasis and lens clarity, muscle sarcoplasmic reticulum Ca(2+)-ATPase was reconstituted with bovine lens lipids and dihydrosphingomyelin, t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0703
更新日期：1999-09-01 00:00:00
abstract：:We sequenced selected peptides of alligator rhodopsin that accounted for about half of the total protein. These sequences were confirmed when the total primary structure of alligator rhodopsin was deduced from the cDNA sequence. Differences in the amino-terminal region, compared to that of bovine rhodopsin, account fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80051-x
更新日期：1995-11-01 00:00:00
abstract：:The effect of a 'soft' anti-inflammatory corticosteroid on the corneal wound healing was investigated in rabbits. Albino rabbits were treated with soft steroid and dexamethasone after linear perforating corneal incision. Soft drug design is aimed to control and direct the drug activity and in vivo metabolism to nontox...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90229-n
更新日期：1990-02-01 00:00:00
abstract：:[3H]-retinol binding to cellular retinol-binding protein (CRBP) increased more than five-fold in developing chicken corneal epithelium at 14 days of incubation, the time when the initial increase in corneal transparency occurs in the developing embryo. In contrast, [3H]-retinoic acid binding to cellular retinoic acid-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90026-x
更新日期：1983-11-01 00:00:00
abstract：:For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.03.027
更新日期：2018-07-01 00:00:00
abstract：:Omental cells (OCs) are shown to help wound healing. The purpose of this study is to investigate if OCs improve cornea repair after alkali injury by subconjunctival injection of activated OCs in rats. Forty eight hours after limbal corneal alkali injury, fresh isolated OCs were injected subconjunctivally into the reci...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.02.014
更新日期：2014-04-01 00:00:00
abstract：:The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2008.09.002
更新日期：2009-05-01 00:00:00
