Abstract：
:The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily through a properly functioning tear film that provides protection, lubrication, and an environment for corneal epithelial cell renewal. LFU cells express thousands of proteins. Over 200 new LFU proteins have been discovered in the last decade. Lacritin is a new LFU-specific growth factor in human tears that flows through ducts to target corneal epithelial cells on the ocular surface. When applied topically in rabbits, lacritin appears to increase the volume of basal tear secretion. Lacritin is one of only a handful of tear proteins preliminarily reported to be downregulated in blepharitis and in two dry eye syndromes. Computational analysis predicts an ordered C-terminal domain that binds the corneal epithelial cell surface proteoglycan syndecan-1 (SDC1) and is required for lacritin's low nanomolar mitogenic activity. The lacritin-binding site on the N-terminus of SDC1 is exposed by heparanase. Heparanase is constitutively expressed by the corneal epithelium and appears to be a normal constituent of tears. Binding triggers rapid signaling to downstream NFAT and mTOR. A wealth of other new proteins, originally designated as hypothetical when first identified by genomic sequencing, are expressed by the human LFU including: ALS2CL, ARHGEF19, KIAA1109, PLXNA1, POLG, WIPI1 and ZMIZ2. Their demonstrated or implied roles in human genetic disease or basic cellular functions are fuel for new investigation. Addressing topical areas in ocular surface physiology with new LFU proteins may reveal interesting new biological mechanisms and help get to the heart of ocular surface dysfunction.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
McKown RL,Wang N,Raab RW,Karnati R,Zhang Y,Williams PB,Laurie GWdoi
10.1016/j.exer.2008.09.002subject
Has Abstractpub_date
2009-05-01 00:00:00pages
848-58issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(08)00317-5journal_volume
88pub_type
杂志文章,评审abstract：:Retinal neovascularization (NV) due to retinal ischemia is one of the major causes of vision reduction in patients with different types of retinal diseases although anti-vascular endothelial growth factor (anti-VEGF) therapy can partially reduce the size of the retinal NV. We recently reported that periostin plays an ...
journal_title：Experimental eye research
pub_type： 杂志文章
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abstract：:In normal retina the developing photoreceptor cells first show presence of opsin over the distal ends of the ciliary protrusions. In a fully differentiated cell intense activity is seen over the rod outer-segment discs; some activity is also seen over the Golgi zone and near the distal ends of the inner segments but t...
journal_title：Experimental eye research
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更新日期：1987-03-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2018-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
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更新日期：1995-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2005-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.008
更新日期：2016-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0626
更新日期：1999-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2012-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2013-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.07.019
更新日期：2009-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.07.005
更新日期：2017-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1984-02-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2003-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.07.020
更新日期：2006-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.10.006
更新日期：2005-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1144
更新日期：1994-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2006-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2019-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1997-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2020-10-01 00:00:00
abstract：:Microaneurysms, acellular capillaries and pericyte ghosts are characteristic of diabetic retinopathy, but it is not clear what causes these lesions or whether they are causally related to each other. The distribution of microaneurysms, acellular capillaries and pericyte ghosts has been evaluated in two animal models o...
journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1995-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107898
更新日期：2020-01-01 00:00:00
abstract：:We previously demonstrated that there are two waves of increased collagen synthesis following corneal laceration in rabbits. In the present study, we have examined whether increases in collagen synthesis and degradation result from increased amounts of mRNAs for collagen and collagenase, respectively. Rabbits were ane...
journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80007-1
更新日期：1988-04-01 00:00:00
