Abstract：
:Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the protein structure of AR. To this end, we have determined the complete protein sequence of rat lens AR using cDNA analysis and primer extension of mRNA. By comparing protein sequences, we have found that the structural relatedness (41% to 57%) among the vertebrate proteins, aldose reductase, aldehyde reductase, prostaglandin F synthase and the frog lens protein rho-crystallin can now be extended to prokaryotes by the inclusion of Corynebacterium 2,5-diketo-D-gluconate reductase. This more distantly related protein shares 30-40% identity with the vertebrate enzymes. Sequence alignments reveal that 18% of the amino acids are completely conserved in all members of the superfamily, many of them in clusters, suggesting that they mark important structural features such as the nucleotide binding site and substrate binding site. rho-Crystallin, which is structurally related to this superfamily of NADPH-dependent reductases, does not appear to reduce PGH2, PGD2, xylose or glyceraldehyde to any appreciable extent. It does, however, bind NADPH.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Carper DA,Wistow G,Nishimura C,Graham C,Watanabe K,Fujii Y,Hayashi H,Hayaishi Odoi
10.1016/0014-4835(89)90048-1subject
Has Abstractpub_date
1989-09-01 00:00:00pages
377-88issue
3eissn
0014-4835issn
1096-0007pii
0014-4835(89)90048-1journal_volume
49pub_type
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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pub_type： 杂志文章
doi：10.1016/0014-4835(84)90116-7
更新日期：1984-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
abstract：:Numerous animal studies indicate that aldose reductase inhibitors (ARIs) are beneficial for the prevention or amelioration of diabetic complications such as neuropathy, nephropathy and the ocular complications of cataract, retinopathy and keratopathy. To aid in the identification of novel potent ARIs, we have previous...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.05.011
更新日期：2004-12-01 00:00:00
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更新日期：2017-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.017
更新日期：2017-11-01 00:00:00
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更新日期：1992-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2021.108450
更新日期：2021-01-23 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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doi：10.1006/exer.1999.0753
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doi：10.1006/exer.1998.0534
更新日期：1998-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2010.01.004
更新日期：2010-05-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1989-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.012
更新日期：2008-02-01 00:00:00
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更新日期：2020-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2013-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90071-4
更新日期：1983-08-01 00:00:00
abstract：:A panel of anti-rhodopsin monoclonal antibodies (MAbs) of defined epitope specificity has been evaluated by immunocytochemistry. Most of the IgG class MAbs (23/27) gave positive results, but only a few of the IgM class MAbs (2/21) were useful for this application. MAbs specific to the N-terminal region stained rod out...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80022-3
更新日期：1989-12-01 00:00:00
