当前位置： SCI文献检索 > EXPERIMENTAL EYE RESEARCH期刊下所有文献 > SPACRCAN in the interphotoreceptor matrix of the mouse retina: molecular, developmental and promoter analysis.

SPACRCAN in the interphotoreceptor matrix of the mouse retina: molecular, developmental and promoter analysis.

Abstract：

:SPACRCAN is a novel proteoglycan present in the interphotoreceptor matrix (IPM) of the rat and human retina that resists aqueous extraction through its binding to hyaluronan. The purpose of this study was: to clone mouse Spacrcan; to characterize the promoter elements; to define the deduced amino acid sequence; to establish the time of Spacrcan expression during retinal development; and to determine the time of appearance and distribution of SPACRCAN protein. Spacrcan cDNA clone was obtained through PCR amplification of a mouse retina cDNA library, and RT-PCR amplification and 5'RACE of mouse retina RNA. The deduced polypeptide sequence of mouse SPACRCAN contains a signal peptide at the N-terminal, seven N-link glycosylation sites, numerous potential O-linked glycosylation sites in a central mucin-like domain, two glycosaminoglycan attachment sites, five potential hyaluronan-binding motifs, two epidermal growth factor-like domains, and a hydrophobic stretch of 23 amino acids near the C-terminal. Comparison of the genomic structure of mouse and human SPACRCAN showed significant structure conservation. Analysis of the promoter region revealed several important putative regulatory elements including a Ret-1/PCE-1 element, an 11 base motif for Crx binding, six copies of PIRE, a Ret-4 element, three copies of AP-1, a CRE element, and five copies of GATA3. Northern blot analysis and immunohistochemistry were used to determine the tissue specificity of Spacrcan mRNA and to localize SPACRCAN in developing retina. Spacrcan mRNA is expressed in both retina and pineal gland and was detectable as early as embryonic day 15. The protein is first detectable in the IPM at postnatal day 8 where it increases in concert with the extension of photoreceptor inner and outer segments from the outer retinal surface. The presence of several unique regulatory elements in the promoter region and characteristic molecular features shared with the orthologue in human and rat suggest an important functional role of SPACRCAN in the IPM. The time of appearance of the SPACRCAN protein during retinal development suggests that this matrix protein may establish the extracellular microenvironment into which photoreceptor outer segments are elaborated.

journal_name

Exp Eye Res

journal_title

Experimental eye research

authors

Chen Q,Lee JW,Nishiyama K,Shadrach KG,Rayborn ME,Hollyfield JG

doi

10.1016/s0014-4835(02)00273-7

keywords:

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

1-14

issue

eissn

0014-4835

issn

1096-0007

pii

S0014483502002737

journal_volume

pub_type

杂志文章

在线工具

SPACRCAN in the interphotoreceptor matrix of the mouse retina: molecular, developmental and promoter analysis.