Abstract：
:We have used a polyclonal antiserum to bovine ciliary epithelium, a secretory tissue involved in the formation of aqueous humor, to immunoscreen a directional lambda gt11 Sfi-Not cDNA expression library prepared from bovine ciliary epithelium poly(A)+ RNA. After immunoscreening 6 x 10(5) independent clones, 41 cDNA clones positive for ciliary epithelium were isolated and characterized. About one-third of the positive cDNA clones were found to be identical and to encode a glutathione S-transferase (GST) class-pi. The largest bovine GST cDNA clone isolated, pCN11, contains an open reading frame of 630 bases, encoding a protein of 210 amino acids with a calculated molecular weight of 23,335 Da. The corresponding amino acid sequence showed an overall identity of 85.6% with the human, and 85.2% with the rat and mouse GST class-pi. Northern analysis of bovine ocular tissues revealed that the GST class-pi gene encodes a 0.8-kilobase mRNA which is expressed most abundantly in cornea, ciliary epithelium and retina, and in lower levels in iris and lens. Cell lines derived from non-pigmented or pigmented bovine ciliary epithelium also showed high levels of GST-pi mRNA expression. These results provide additional evidence for differential gene expression of GST class-pi mRNA in various areas of the bovine eye.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Hernando N,Martín-Alonso JM,Ghosh S,Coca-Prados Mdoi
10.1016/0014-4835(92)90175-rkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
711-8issue
5eissn
0014-4835issn
1096-0007journal_volume
55pub_type
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：1997-06-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2004-02-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1987-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/exer.1994.1193
更新日期：1994-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1083
更新日期：1994-07-01 00:00:00
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更新日期：1983-03-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0869
更新日期：2000-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1994-05-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2017-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90054-v
更新日期：1992-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.05.011
更新日期：2012-08-01 00:00:00
abstract：:In order to develop effective optical treatment strategies for myopia, it is important to understand how visual experience influences refractive development. Beginning with the discovery of the phenomenon of form deprivation myopia, research involving many animal species has demonstrated that refractive development is...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2012.11.019
更新日期：2013-09-01 00:00:00
