Abstract：
:Cultured bovine lens epithelial cells adapt to hypertonic sodium stress via an increase in Na(+)-myo-inositol cotransporter activity and accumulate myo-inositol. At least 12 hr of hypertonic exposure was necessary to enhance myo-inositol accumulation; and thereafter, uptake activity continued to increase throughout the duration of a 72-hr exposure period. Switching from hypertonic to isotonic medium for 24 hr reversed the otherwise elevated accumulation activity. The protein synthesis inhibitor, cycloheximide, did not affect myo-inositol uptake in isotonic medium but markedly decreased myo-inositol uptake in hypertonic medium. Cells exposed to hypertonic conditions and the microtubule disrupter, colchicine, similarly showed marked impairment of the otherwise enhanced myo-inositol uptake. These studies indicated that hypertonicity-induced elevation of Na(+)-myo-inositol cotransporter activity in cultured bovine lens epithelial cells is not solely attributed to the increased sodium gradient alone, but rather involves increased de novo synthesis of the Na(+)-myo-inositol cotransporter protein(s).
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Cammarata PR,Chen HQ,Zhou C,Reeves Rdoi
10.1006/exer.1994.1083subject
Has Abstractpub_date
1994-07-01 00:00:00pages
83-9issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71083-9journal_volume
59pub_type
杂志文章abstract：:Several scleral cross-linking (SXL) methods were suggested to increase the biomechanical stiffness of scleral tissue and therefore, to inhibit axial eye elongation in progressive myopia. In addition to scleral cross-linking and biomechanical effects caused by riboflavin and light irradiation such a treatment might ind...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.07.005
更新日期：2015-10-01 00:00:00
abstract：:Hypoxic preconditioning protects photoreceptors against light-induced degeneration preserving retinal morphology and function. Although hypoxia inducible transcription factors 1 and 2 (HIF1, HIF2) are the main regulators of the hypoxic response, photoreceptor protection does not depend on HIF1 in rods. Here we used ro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.12.008
更新日期：2016-05-01 00:00:00
abstract：:A prior study found that inhibition of nitric oxide synthase with L-NAME causes a large, rapid decrease in IOP in anesthetized rabbits. In this follow-up study we sought to determine if this hypotensive effect was due to decreased aqueous production, possibly caused by ciliary vasoconstriction. Two protocols were perf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1050
更新日期：2001-09-01 00:00:00
abstract：:The present study was undertaken to develop a new animal model of subretinal neovascularization that does not involve traumatic manipulation of the eye. Using this model, the mechanism of subretinal neovascularization and its penetration through Bruch's membrane, and the various factors that contribute to this process...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1003
更新日期：1994-02-01 00:00:00
abstract：:The purpose of this study was to investigate the impact of different diffusion times of mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. Scleral quadrants of eight human donor eyes were exposed to sponges soaked with MMC for an application time of 1 min. After irrig...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0901
更新日期：2000-11-01 00:00:00
abstract：:The elimination of spermine, the end product of cellular polyamine, from the retina to the blood across the blood-retinal barrier (BRB) was investigated. The in vivo microdialysis study revealed that the elimination of [(3)H]spermine from vitreous humor after vitreous bolus injection was in a biexponential manner. The...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) was studied using immunochemical and immunocytochemical techniques in retinae of mice with allelic combinations at the rd and rds loci at different stages of development and degeneration. Until postnatal day 7 (P7), IRBP is located intracellularly in developing retina...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90012-7
更新日期：1988-08-01 00:00:00
abstract：:γ-secretase inhibitor has been shown to promote intestinal goblet cell differentiation. We now demonstrated that the in vitro addition of γ-secretase inhibitor in the culture of human conjunctival epithelial cells significantly promoted the differentiation of conjunctival goblet cells with typical droplet-like phenoty...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.04.001
更新日期：2014-06-01 00:00:00
abstract：:The majority of dry eye symptoms are due to a chronic inflammation of the lacrimal functional unit resulting in a loss of tear film integrity and normal function. This leads to a reduction in the ability of the ocular surface to respond to environmental challenges. The underlying cause of tear film dysfunction is the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.09.003
更新日期：2004-03-01 00:00:00
abstract：:The purpose of this investigation is to characterize parvalbumin-immunoreactive (IR) neurons in the inner nuclear layer (INL) of zebrafish retina through immunocytochemistry, quantitative analysis, and confocal microscopy. In the INL, parvalbumin-IR neurons were located in the inner marginal portion of the INL. On the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.11.014
更新日期：2009-03-01 00:00:00
abstract：:A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80067-3
更新日期：1989-02-01 00:00:00
abstract：:Myopia is the most common form of refractive eye disease, and the prevalence is increasing rapidly worldwide. However, the key metabolic alterations in individuals with high myopia are not understood clearly, and serum biomarkers remain to be determined. The objectives of this study were to identify serum biomarkers a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107737
更新日期：2019-09-01 00:00:00
abstract：:Corneal epithelial defects are a common cause of ocular morbidity and can result in corneal scarring if they do not heal properly. Matrix metalloproteinases (MMPs) are extracellular matrix proteinases that regulate multiple aspects of corneal repair. We have previously shown that MMP12 has a protective effect on corne...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.04.007
更新日期：2017-07-01 00:00:00
abstract：:Phosducin is a retinal and pineal phosphoprotein assumed to play an important role in visual phototransduction. Phosducin is also a uveitopathogenic retinal antigen, but its potency has been reported to be mild. During the course of studies aimed at identifying uveitopathogenic sites in phosducin, we found that rat ph...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0379
更新日期：1997-11-01 00:00:00
abstract：:The state of water and water fractions in normal and senile cataractous eye lenses was studied by the NMR method. Combining NMR with vacuum dehydration provided additional information on multifractional samples. A new mathematical procedure is presented which separates the characteristic parameters of the different fr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90104-5
更新日期：1983-05-01 00:00:00
abstract：:There has been generally little attention paid to the utilization of biomaterials as an anti-myopia treatment. The purpose of this study was to investigate whether polymeric hydrogels, either implanted or injected adjacent to the outer scleral surface, slow ocular elongation. White Leghorn (Gallus gallus domesticus) c...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.10.029
更新日期：2009-03-01 00:00:00
abstract：:A lateral potential drop along the outer surface of the cornea could be measured at the edge of wounds made in the corneal epithelium of the isolated bovine eye when the cornea was covered by simulated tear film of modified Hanks' solution. These lateral fields (LFs) had an average magnitude of 42 +/- 1.4 mV mm-1 in t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90164-n
更新日期：1992-06-01 00:00:00
abstract：:Macular corneal dystrophy (MCD) is an autosomal recessive disease characterized by abnormal deposition of glycosaminoglycans in corneal stroma, keratocytes, Descemet's membrane and corneal endothelium. According to the presence and distribution of sulfated keratan sulfate (KS)-epitopes in serum and cornea (using mAb 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1080
更新日期：2001-11-01 00:00:00
abstract：:Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvast...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.05.013
更新日期：2013-08-01 00:00:00
abstract：:Selective pericyte loss, the histological hallmark of early diabetic retinopathy (DR), enhances the breakdown of the blood-retinal barrier (BRB) in diabetes. However, the role of pericytes on BRB alteration in diabetes and the signaling pathways involved in their effects are currently unknown. To understand the role o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108043
更新日期：2020-06-01 00:00:00
abstract：:This study aimed to assess the cytotoxic effect of low molecular weight components (LMWC) and conventional silicone oils (SOs) 1000 cSt with different degree of purification (raw, intermediate, and purified) using in vitro cytotoxicity tests. Direct contact cytotoxicity tests were performed in BALB 3T3 and human retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108018
更新日期：2020-05-01 00:00:00
abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0069
更新日期：1996-06-01 00:00:00
abstract：:The purpose of these experiments was to develop a hypothesis to explain activation of m-calpain in cataractogenesis observed in rodents. The in vitro model used to study m-calpain activation was to correlate breakdown of the 'reporter' protein alpha-crystallin with the appearance of activated m-calpain using protein s...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0234
更新日期：1997-04-01 00:00:00
abstract：:A number of different agents, such as growth factors, cytokines and phorbol esters have been shown to modulate trabecular meshwork cell function. These studies were designed to evaluate the role extracellular signal-regulated kinase (ERK) pathway plays in mediating the responses to platelet-derived growth factor-BB (P...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1007
更新日期：2001-07-01 00:00:00
abstract：:Age-related Macular Degeneration (AMD) is the leading cause of visual impairment and blindness in the elderly in developed countries. Neovascular/exudative (wet) AMD is the aggressive form of AMD and can involve choroidal neovascularization and vascular leakage. Anti-vascular endothelial growth factor (anti-VEGF) medi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.010
更新日期：2016-04-01 00:00:00
abstract：:The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0047
更新日期：1996-04-01 00:00:00
abstract：:Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.08.013
更新日期：2019-06-01 00:00:00
abstract：:Transmural electrical properties of the isolated rabbit iris-ciliary body (I-CB) were measured in Ussing-Zerahn-type chambers. Control p.d. across the preparation was -1.2 +/- 0.1 mV, with the ciliary process (aqueous)-side consistently negative with respect to the ciliary body (blood)-side and the short-circuit curre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90096-4
更新日期：1984-02-01 00:00:00
abstract：:Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprag...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.12.001
更新日期：2011-03-01 00:00:00
abstract：:The interphotoreceptor matrix (IPM) is a highly organized structure with interconnected domains surrounding cone and rod photoreceptor cells and extends throughout the subretinal space. Based on known roles of the extracellular matrix in other tissues, the IPM is thought to have several prominent functions including s...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.02.017
更新日期：2015-04-01 00:00:00
