Inductive differentiation of conjunctival goblet cells by γ-secretase inhibitor and construction of recombinant conjunctival epithelium.

abstract：:Epidermal growth factor (EGF) previously has been shown to stimulate short-term survival in vitro of cells derived from the native amphibian retinal pigment epithelium (RPE). In the present experiments, we have examined intracellular signaling pathways responsible for mediating these survival-specific growth factor ef...

journal_title：Experimental eye research

authors： Defoe DM,Grindstaff RD

更新日期：2004-07-01 00:00:00

abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...

journal_title：Experimental eye research

authors： Cabañas ME,Alonso J,Arús C,Anderson ME,Westler WM,Markley JL

更新日期：1993-12-01 00:00:00

abstract：:In the ophthalmological clinic, intravitreally applied antibodies or Fc-containing fusion proteins are frequently used, but the biology and pharmacokinetics of these therapeutics in the retina are not well understood. We have previously shown intracellular uptake of Fc-containing molecules in RPE cells. In this study,...

journal_title：Experimental eye research

authors： Dithmer M,Hattermann K,Pomarius P,Aboul Naga SH,Meyer T,Mentlein R,Roider J,Klettner A

更新日期：2016-04-01 00:00:00

abstract：:In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this s...

journal_title：Experimental eye research

authors： Wang Z,Sosne G,Kurpakus-Wheater M

更新日期：2005-01-01 00:00:00

abstract：:We studied a new fluorophotometric method to determine trabecular outflow facility in man. Pilocarpine was selected to evaluate this non-invasive method, since its primary ocular hypotensive effect is to increase trabecular outflow facility. Levobunolol was used to decrease intraocular pressure and aqueous humor flow ...

journal_title：Experimental eye research

authors： Hayashi M,Yablonski ME,Novack GD

更新日期：1989-05-01 00:00:00

abstract：:Certain mutations in optineurin (gene OPTN) are associated with primary open angle glaucoma. Optineurin is ubiquitously expressed but it shows high level of expression in certain cells and tissues including retinal ganglion cells. It interacts with many proteins, often acting as an adaptor to link two or more proteins...

journal_title：Experimental eye research

authors： Sirohi K,Swarup G

更新日期：2016-03-01 00:00:00

abstract：:Various concentrations of three dopamine antagonists (fluphenazine, haloperidol and sulpiride) with different affinities to the receptor subtypes were applied in order to test their effect on responses from outer (isolated PIII-component of the ERG), middle (b-wave) and inner (optic nerve response, ONR) retinal layers...

journal_title：Experimental eye research

authors： Schneider T,Zrenner E

更新日期：1991-04-01 00:00:00

abstract：:Thioltransferase (TTase) activity was identified and partially purified from the ocular tissue for the first time. The enzyme activity depended on the presence of reduced glutathione (GSH), glutathione reductase (GR) and NADPH to reduce the disulfide bond in a synthetic substrate, hydroxyl ethyl disulfide (HEDS). Maxi...

journal_title：Experimental eye research

authors： Raghavachari N,Lou MF

更新日期：1996-10-01 00:00:00

abstract：:The part of the light spectrum which is responsible for an increase of lenticular green autofluorescence upon white light exposure was determined for the rabbit. The increase of autofluorescence as a function of the light energy was measured, to assess a possible threshold energy for lens alterations. Thirteen rabbits...

journal_title：Experimental eye research

authors： Van Vreeswijk H,Boets EP,Van Best JA

更新日期：1993-03-01 00:00:00

abstract：:In normal retina the developing photoreceptor cells first show presence of opsin over the distal ends of the ciliary protrusions. In a fully differentiated cell intense activity is seen over the rod outer-segment discs; some activity is also seen over the Golgi zone and near the distal ends of the inner segments but t...

journal_title：Experimental eye research

authors： Jansen HG,Sanyal S,De Grip WJ,Schalken JJ

更新日期：1987-03-01 00:00:00

abstract：:Utilizing a human beta-actin promoter, a catalase cDNA expression vector was constructed. This construct was used to transfect two immortal cell lines, mouse alpha TN4-1 and rabbit N/N 1003A. The catalase activity was increased about 3.4 fold in the alpha TN4-1 cells and 38 fold in the N/N 1003A cells. Some changes in...

journal_title：Experimental eye research

authors： Yang Y,Spector A,Ma W,Wang RR,Larsen K,Kleiman NJ

更新日期：1998-12-01 00:00:00

abstract：:The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...

journal_title：Experimental eye research

authors： McKown RL,Wang N,Raab RW,Karnati R,Zhang Y,Williams PB,Laurie GW

更新日期：2009-05-01 00:00:00

abstract：:A small percentage of retinal ganglion cells (RGCs) express melanopsin and are intrinsically photosensitive (ipRGCs). Whether light can affect the development of ipRGCs is not clear. In the rat retina, we found constant light exposure during the first postnatal week significantly increased the number of melanopsin imm...

journal_title：Experimental eye research

authors： Hong J,Zeng Q,Wang H,Kuo DS,Baldridge WH,Wang N

更新日期：2013-06-01 00:00:00

abstract：:Ciliary ganglionectomy inhibits the development of myopia in chicks (Schmid et al., 1999), but has no effect on the compensatory responses to spectacle lenses (Schmid and Wildsoet, 1996). This study was done to assess the potential influence of the other parasympathetic input to the choroid, the pterygopalatine gangli...

journal_title：Experimental eye research

authors： Nickla DL,Schroedl F

更新日期：2012-09-01 00:00:00

abstract：:The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...

journal_title：Experimental eye research

authors： Blanks JC,Spee C

更新日期：1992-05-01 00:00:00

abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...

journal_title：Experimental eye research

authors： Stevens A,Augusteyn RC

更新日期：1988-04-01 00:00:00

abstract：:Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...

journal_title：Experimental eye research

authors： Wu W,Tholozan FM,Goldberg MW,Bowen L,Wu J,Quinlan RA

更新日期：2014-03-01 00:00:00

abstract：:The passage of glucose within the cornea has been thought to occur by passive diffusion processes. However, corneal glucose concentration profiles have not been established to support this notion. While microfluorometric methods of metabolite assay typically have been used as a means of assessing regional brain metabo...

journal_title：Experimental eye research

authors： Lattimore MR Jr

更新日期：1988-11-01 00:00:00

abstract：:Recent epidemiological evidence in children indicates that time spent outdoors is protective against myopia. Studies in animal models (chick, macaque, tree shrew) have found that light levels (similar to being in the shade outdoors) that are mildly elevated compared to indoor levels, slow form-deprivation myopia and (...

journal_title：Experimental eye research

authors： Norton TT,Siegwart JT Jr

更新日期：2013-09-01 00:00:00

abstract：:The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...

journal_title：Experimental eye research

authors： Yoo YS,Na KS,Kim DY,Yang SW,Joo CK

更新日期：2017-10-01 00:00:00

abstract：:Alpha B-crystallin, a major lens protein, was induced in primary cultures of dog lens epithelial cells and glomerular endothelial cells when they were grown under conditions of hypertonic stress. With Western blot analysis using a specific alpha B-crystallin antibody, we observed a significant increase in the concentr...

journal_title：Experimental eye research

authors： Dasgupta S,Hohman TC,Carper D

更新日期：1992-03-01 00:00:00

abstract：:Fungal keratitis is a common disease that causes blindness. An effective animal model for fungal keratitis is essential for advancing research on this disease. Our objective is to develop a novel mouse model of Fusarium solani keratitis through the inoculation of fluorescent-labeled fungi into the cornea to facilitate...

journal_title：Experimental eye research

authors： Zhang H,Wang L,Li Z,Liu S,Xie Y,He S,Deng X,Yang B,Liu H,Chen G,Zhao H,Zhang J

更新日期：2013-05-01 00:00:00

abstract：:Electrical field stimulation produced contractions of the rabbit isolated iris sphincter muscle which were completely blocked by tetrodotoxin (10(-6) M). Low frequency (0.16-5 Hz) responses were atropine-resistant, but were prevented by sensory (trigeminal) denervation, and were considered to be owing to antidromic st...

journal_title：Experimental eye research

authors： Zhang SQ,Butler JM,Cole DF

更新日期：1984-02-01 00:00:00

abstract：:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...

journal_title：Experimental eye research

authors： Cilluffo MC,Fain MJ,Fain GL

更新日期：1993-11-01 00:00:00

abstract：:The objective of the study was to investigate the effects of baicalin, baicalein, and wogonin (plant flavonoids) on interleukin-6 (IL-6) and interleukin-8 (IL-8) protein production, mRNA expression, and nuclear factor-kappaB (NF-kappaB) binding activities induced by interleukin-1beta (IL-1beta) in human retinal pigmen...

journal_title：Experimental eye research

authors： Nakamura N,Hayasaka S,Zhang XY,Nagaki Y,Matsumoto M,Hayasaka Y,Terasawa K

更新日期：2003-08-01 00:00:00

abstract：:Myopia is one of the leading ocular disorders causing visual impairment worldwide, with the prevalence increasing rapidly. It's a significant global public health concern in 21st century. Myopia, particularly high myopia, often exhibits apparent familial aggregation, and multiple evidences have shown that genetic fact...

journal_title：Experimental eye research

authors： Cai XB,Shen SR,Chen DF,Zhang Q,Jin ZB

更新日期：2019-11-01 00:00:00

abstract：:Both in vivo and in vitro models were employed in the present study to assess the relative contribution of osmotic stress and increasing calcium levels to the development of sugar cataracts. In galactose cataract obtained from galactosemic weanling rats, the concentration of total calcium increased by nearly 10% at th...

journal_title：Experimental eye research

authors： Hightower KR,Misiak P

更新日期：1998-06-01 00:00:00

abstract：:Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treat...

journal_title：Experimental eye research

authors： Boros S,Wilmarth PA,Kamps B,de Jong WW,Bloemendal H,Lampi K,Boelens WC

更新日期：2008-02-01 00:00:00

abstract：:Keratoconus is a debilitating corneal thinning disease that principally develops in the second and third decades of life. Our group previously developed a novel approach to studying keratoconus, based on the observation that there is a gradient of damage across the keratoconic cone. We identified a number of cellular ...

journal_title：Experimental eye research

authors： Brookes NH,Loh IP,Clover GM,Poole CA,Sherwin T

更新日期：2003-10-01 00:00:00

abstract：:The irritative response to Nd:YAG laser capsulotomy was studied in unanaesthetized rabbits. Posterior lens capsulotomy with a total energy of 100 mJ had no effect on the pupil size but increased the intraocular pressure by 5-10 mmHg and caused a breakdown of the blood-aqueous barrier. Anterior lens capsulotomy with a ...

journal_title：Experimental eye research

authors： Stjernschantz J,von Dickhoff K,Oksala O,Seppä H

更新日期：1986-10-01 00:00:00