Abstract：
:A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages through an Agarose A-1.5 m column. The purified proteinase exhibited molecular weight of 38 kDa on a SDS-polyacrylamide gel. A polyclonal antiserum was raised against bovine lens membrane proteinase, and used as a probe to examine distribution of the enzyme among water-soluble and insoluble proteins of human lenses of different ages. The antiserum had strict specificity to the human membrane proteinase as it showed immunoreaction to only the proteinase among human membrane proteins and crystallins. In addition, the antiserum also inhibited the proteinase activity on incubation. The Western blot of water-soluble proteins from 2-yr-old lens showed a 22 kDa immunoreactive protein, but an additional protein of 43 kD in lenses older than 19 years was observed. A similar Western blot analysis of the water-insoluble proteins from these lenses showed a single protein of 18 kDa that was identified as the subunit of the bovine lens membrane proteinase (Srivastava, 1988a). Furthermore, the immunoreactive 18 kD protein of the water-insoluble protein fractions could be solubilized with urea. The proteinase activity was found to increase with aging, as judged by the extraction of the enzyme with 2% deoxycholate from membranes of lenses of different ages and proteinase activity determination. Similarly, an age-related increase in the immunoreaction was also observed on measuring radio-iodinated protein A bound to an immunoreactive 18 kD protein of the water-insoluble protein fractions.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Srivastava OP,Srivastava Kdoi
10.1016/s0014-4835(89)80067-3subject
Has Abstractpub_date
1989-02-01 00:00:00pages
161-75issue
2eissn
0014-4835issn
1096-0007journal_volume
48pub_type
杂志文章abstract：:A newly-developed fibre-optic sensor (wavelength of 670 nm) was used to measure the index gradient in 16 human lenses ranging in age from 38 to 78 years. Two of these lenses had nuclear cataract. The refractive index, calculated from the proportion of reflected light at the sensor/sample interface, appeared to increas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80097-1
更新日期：1995-04-01 00:00:00
abstract：:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0765
更新日期：2000-02-01 00:00:00
abstract：:Choroidal neovascularization (CNV) is one of the severe pathological consequences of the end-stage of age-related macular degeneration (AMD). Several lines of evidence implicate increased levels of vascular endothelial growth factor (VEGF) in the retinas of AMD patients. Current available agents for the inhibition of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.06.019
更新日期：2010-09-01 00:00:00
abstract：:The purpose of this study was to investigate the roles of endothelium-derived factors in the retinal arteriolar responses to acute severe elevation in systemic blood pressure (BP) in cats. Acute elevation of mean arterial BP by 60% for 5 min was achieved by inflating a balloon-tipped catheter in the descending aorta. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.08.007
更新日期：2012-10-01 00:00:00
abstract：:The purpose of the current study was to localize the lens membrane protein, MIP 26, in nuclear fiber cells from different regions of aged normal and age-related cataractous human lenses. Adult, juvenile, fetal and embryonic nuclear regions in aged normal and age-related nuclear cataractous lenses were morphologically ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0579
更新日期：1999-01-01 00:00:00
abstract：:Dysfunctional vascular endothelial nitric oxide synthase (eNOS) has been proposed to play a main pathophysiological role in various ocular diseases. The aim of the present study was to test the hypothesis that the chronic lack of eNOS impairs endothelium-dependent vasodilation in retinal arterioles. The relevance of e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.022
更新日期：2019-04-01 00:00:00
abstract：:The ability of interphotoreceptor retinoid-binding protein (IRBP) to facilitate the incorporation of retinol into retinyl esters by the retinal pigment epithelium (RPE) was examined in toad (Bufo marinus) eyecup preparations devoid of neural retina (RPE-eyecup). Solutions containing purified bovine IRBP and all-trans[...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80059-4
更新日期：1989-10-01 00:00:00
abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.exer.2010.03.008. The duplicate article has therefore been withdrawn. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.07.012
更新日期：2011-07-29 00:00:00
abstract：:A technology has recently been developed that allows for the rapid transduction of full-length functionally active proteins into intact tissue through intravenous injection and into cultured cells. This technology involves the fusion of an 11 amino acid sequence of the HIV TAT protein to the protein of interest. In th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.12.010
更新日期：2004-05-01 00:00:00
abstract：:Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90150-s
更新日期：1990-10-01 00:00:00
abstract：:The expression and intracellular localization of the estrogen receptor (ER) subtypes, ERalpha and ERbeta were examined in cultured human lens epithelial cells (HLE-B3). ERalpha and ERbeta mRNA expression was evaluated by coupled reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Subce...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.027
更新日期：2004-04-01 00:00:00
abstract：:Suzuki et al. [Vis. Res. 26, 425-9 (1986); Vis. Res. 28, 1061-70 (1988)] have described a formaldehyde-based (HCHO-based) extraction procedure that efficiently recovers 11-cis retinal initially present as rhodopsin chromophore in photoreceptor membranes. Using the isolated retina of the toad (Bufo marinus), we tested ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0331
更新日期：1997-09-01 00:00:00
abstract：:miRNA-7a plays critical roles in various biological aspects in health and disease. We aimed to reveal roles of miR-7a in mouse retinal development by loss- and gain-of-function analyses of miR-7a. Plasmids encoding miR-7a or miR-7a-decoy (anti-sense miR-7a) were introduced into mouse retina at P0, and the retina was c...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.022
更新日期：2015-09-01 00:00:00
abstract：:Corneal epithelial (CE) cells are exposed to environmental insults (e.g., UV-irradiation), yet they suffer little damage. Our previous studies suggest that chicken CE cells have a novel form of protection involving having ferritin in a nuclear location where it can bind to DNA and sequester free iron. Here we describe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.002
更新日期：2016-04-01 00:00:00
abstract：:The proliferation, migration and transdifferentiation of the remaining lens epithelial cells (LECs) after cataract surgery are a major cause of posterior capsular opacification (PCO). It has previously been reported that salmosin, a novel disintegrin, significantly inhibits solid tumor growth in mice by perturbation o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1150
更新日期：2002-05-01 00:00:00
abstract：:Careful control of iron availability in the retina is central to maintenance of iron homeostasis, as its imbalance is associated with oxidative stress and the progression of several retinopathies. Ferritin, known for its role in iron storage and detoxification, has also been proposed as an iron-transporter protein, th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108292
更新日期：2021-01-01 00:00:00
abstract：:The present study was designed to identify genomic loci modulating the susceptibility of retinal ganglion cells (RGC) to elevated intraocular pressure (IOP) in the BXD recombinant inbred mouse strain set. IOP was elevated by injecting magnetic microspheres into the anterior chamber and blocking the trabecular meshwork...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.12.013
更新日期：2018-04-01 00:00:00
abstract：:Prostaglandins are widely used to lower intraocular pressure (IOP) as part of the treatment regimen for glaucoma. While FP and EP2 agonists are known to lower IOP, we investigated the ocular hypotensive activity and ocular drug distribution of PF-04475270, a novel EP4 agonist following topical administration in normot...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.05.002
更新日期：2009-11-01 00:00:00
abstract：:Early-onset Fuchs endothelial corneal dystrophy (FECD) has been associated with nonsynonymous mutations in collagen VIII α2 (COL8A2), a key extracellular matrix (ECM) protein in Descemet's membrane (DM). Two knock-in strains of mice have been generated to each express a mutant COL8A2 protein (Col8a2L450W/L450W and Col...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.11.021
更新日期：2019-03-01 00:00:00
abstract：PURPOSE:To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates. METHODS:Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.013
更新日期：2004-01-01 00:00:00
abstract：:There is increasing evidence implicating Transforming growth factor beta (TGF-beta) in pathological states of the lens. However, the underlying signalling mechanisms in human cells have not been fully examined. We have therefore investigated in a human lens cell line, FHL 124, the signalling characteristics of TGF-bet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.08.006
更新日期：2004-03-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) greatly enhances the conversion of all- trans -retinol to 11- cis -retinal by the retinal pigment epithelium (RPE) and facilitates 11- cis -retinal release from the RPE. However, the mechanisms by which IRBP exerts these effects are not clear. Using a model system of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0781
更新日期：2000-02-01 00:00:00
abstract：:Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas of SDS gels (Western blots) were used in a developmental study to detect the presence and localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their BALB/c normal controls. Western blot analysis of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80061-1
更新日期：1987-10-01 00:00:00
abstract：:The autonomic innervation in the anterior chamber (AC) structures might play an efferent role in neural intraocular pressure (IOP) regulation, the center of which is thought to be located in the hypothalamus. In this study, we identified the efferent pathway from the hypothalamus to the autonomic innervation in the AC...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108367
更新日期：2021-01-01 00:00:00
abstract：:The effects of temperature on rod outer segment (ROS) length and membrane shedding were studied in a cyprinodont fish, Fundulus zebrinus. After 30 days in 14L/10D cyclic light and 17 degrees C, ROS length averaged 41.2 microns. Fish were then exposed to 7, 17 or 27 degrees C for 10 and 25 days before being sampled 5 h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80036-3
更新日期：1995-08-01 00:00:00
abstract：:Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.08.013
更新日期：2019-06-01 00:00:00
abstract：:To trace the path taken by the putative postganglionic secretomotor fibres to the lacrimal gland the contents of the orbital and pterygopalatine fossa were removed whole, cut coronally into slabs and embedded in resin. Thin sections were cut at varying intervals to reconstruct the pathway taken. One group of rami orbi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.06.001
更新日期：2004-03-01 00:00:00
abstract：:The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0212
更新日期：1997-03-01 00:00:00
abstract：:A prior study found that inhibition of nitric oxide synthase with L-NAME causes a large, rapid decrease in IOP in anesthetized rabbits. In this follow-up study we sought to determine if this hypotensive effect was due to decreased aqueous production, possibly caused by ciliary vasoconstriction. Two protocols were perf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1050
更新日期：2001-09-01 00:00:00
abstract：:Recent studies indicate a key role of Mitf (microphthalmia-associated transcription factor) in the differentiation of the retinal pigment epithelium (RPE). To explore transdifferentiation processes, we used small interfering RNA (siRNA) targeting Mitf. Transfection of embryonic chick RPE cells with a non-silencing flu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.004
更新日期：2005-07-01 00:00:00
