Abstract：
:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-regulated in the retina-RPE-choroid of form-deprived chick eyes. A suppression subtractive hybridization method was used to compare mRNA expression in the retina-RPE-choroid of form-deprived and control eyes. One up-regulated cDNA was isolated and identified as part of chick delta1-crystallin cDNA. Northern blot and RT-PCR analyses demonstrated that delta1-crystallin mRNA was up-regulated in the retina-RPE at day 7 after form-deprivation treatment. Semi-quantitative RT-PCE analysis of the expression of several transcription factors indicated that Sox1 and Sox3 were upregulated in parallel with delta1-crystallin mRNA in form-deprived eyes. Northern blot analysis demonstrated that alphaA-, betaA3/A1-, betaB1-, and betaB2-crystallin mRNAs were also up-regulated in form-deprived eyes. Although the detailed mechanisms and functions of the crystallin family genes in the retina-RPE-choroid of form-deprived eyes remain unclear, results of our study suggests that form-deprivation affects the expression of these genes in chick eyes.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Ishibashi K,Fujii S,Escaño MF,Sekiya Y,Yamamoto Mdoi
10.1006/exer.1999.0765keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
153-8issue
2eissn
0014-4835issn
1096-0007pii
S0014-4835(99)90765-0journal_volume
70pub_type
杂志文章abstract：:This study demonstrates post-translational arginylation of bovine serum albumin and endogenous lens proteins by bovine lens arginyl-tRNA:protein transferase. This reaction has been proposed to be the first step in marking specific proteins for degradation by the non-lysosomal, ATP-dependent, ubiquitin-mediated proteol...
journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
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pub_type： 杂志文章
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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pub_type： 杂志文章
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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