Abstract：
:The aim of this study was to elucidate the expression of chemokines, their role and regulation in bacterial corneal infection using three bacterial strains (Pseudomonas. aeruginosa- invasive, cytotoxic and contact lens induced acute red eye strains) which have been shown to produce three distinct patterns of corneal disease in the mouse. The predominant chemokine expressed in response to all three strains was MIP-2. Prolonged expression of high levels of MIP-2 was associated with increased severity of corneal inflammation. Significantly reduced disease severity upon administration of anti-MIP-2 antibodies suggested that MIP-2 may play an important role in the pathogenesis of Pseudomonas keratitis at least in part by being a major chemoattractant for polymorphonuclear leukocytes (PMN) recruitment. Interestingly, the numbers of bacteria in eyes with neutralized MIP-2 activity did not decrease even though the severity of the disease was decreased. This implies PMNs as the major destructive factor in microbial keratitis. Further, neutralization of IL-1beta activity alone using monoclonal antibodies resulted in significant reduction of both MIP-2 and KC activity indicating that chemokine levels were regulated by IL-1beta. These studies demonstrate that the regulation of MIP-2 activity may be beneficial in reducing corneal damage during microbial keratitis in rodents and perhaps that regulation of the human homologue of MIP-2, IL-8, may be useful for controlling keratitis in humans.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Xue ML,Thakur A,Willcox MD,Zhu H,Lloyd AR,Wakefield Ddoi
10.1016/s0014-4835(02)00270-1keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
221-31issue
2eissn
0014-4835issn
1096-0007pii
S0014483502002701journal_volume
76pub_type
杂志文章abstract：:Aim of present investigation was to study the effect of catechin and the combination of catechin and cetirizine in ovalbumin induced animal model of allergic conjunctivitis. Guinea pigs were divided into 5 groups: normal control, disease control, disease treated with catechin 100 mg/kg, disease treated with cetirizine...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.05.020
更新日期：2015-08-01 00:00:00
abstract：:There is increasing evidence implicating Transforming growth factor beta (TGF-beta) in pathological states of the lens. However, the underlying signalling mechanisms in human cells have not been fully examined. We have therefore investigated in a human lens cell line, FHL 124, the signalling characteristics of TGF-bet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.08.006
更新日期：2004-03-01 00:00:00
abstract：:Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprag...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.12.001
更新日期：2011-03-01 00:00:00
abstract：:Corneal endothelium appears to be an important target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid, or its synthetic analogue CBS-211 A (10(-8)-10(-7) M) dose-dependently enhances the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90169-u
更新日期：1990-07-01 00:00:00
abstract：:Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stain...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90111-8
更新日期：1984-07-01 00:00:00
abstract：:Traumatic optic neuropathy (TON) can occur following blunt trauma to the orbit and can lead to permanent vision loss. In this study, we investigated the effectiveness of elamipretide (MTP-131), a small mitochondrially-targeted tetrapeptide, in conjunction with etanercept, a tumor necrosis factor (TNF) inhibitor, as ne...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.108178
更新日期：2020-10-01 00:00:00
abstract：:Long living animal models of retinitis pigmentosa (RP) can provide important information on the retinal changes that occur at the late stages of photoreceptor degeneration. The rhodopsin Pro347Leu transgenic rabbit (P347L Tg) is a model of RP, and it has been used to analyze the functional and morphological changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.026
更新日期：2019-07-01 00:00:00
abstract：:This review describes our recent attempts to develop new therapeutic modalities for corneal endothelial disease using animal models including non-human primate model in which the proliferative ability of corneal endothelial cells is severely limited, as is the case in humans. First, we describe our attempt to develop ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2011.10.014
更新日期：2012-02-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:Vitreous from bovine, human and chick embryo has been found to contain a trypsin inhibitory activity. Chymotrypsin-inhibitory activity was also identified in bovine and chick embryo vitreous. Following either ultrafiltration or Bio Gel P-10 chromatography, these activities appear in fractions having a molecular weight...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90031-4
更新日期：1985-11-01 00:00:00
abstract：:Erythropoietin (EPO) plays a critical role in the vascular system and exhibits angiogenic activity in endothelial cells (ECs) such as stimulation of cell proliferation, migration and tube formation in vitro. EPO is the major regulator of cell proliferation and differentiation of erythroid precursors and there by preve...
journal_title：Experimental eye research
pub_type： 杂志文章,收录出版
doi：10.1016/j.exer.2010.03.005
更新日期：2010-06-01 00:00:00
abstract：:Rabbit immunoglobulin G (IgG) binds specifically to the human corneal epithelium. We investigated this phenomenon to identify the binding material in the tissue. Sections of human cornea were immunostained with negative-control grade rabbit IgG, F(ab')(2) (the antigen recognition fragment of IgG yielded by pepsin dige...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.01.006
更新日期：2004-06-01 00:00:00
abstract：:An attachment to (Haag-Streit and Topcon) slit-lamps is described. This permits projection of a patterned target into the eye and the visual or photographic analysis of the details of the image reflected from the posterior surface of the crystalline lens. A numerical assessment can then be made of the optical quality ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90124-b
更新日期：1992-09-01 00:00:00
abstract：:An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation. Using FV...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.01.021
更新日期：2007-06-01 00:00:00
abstract：:A number of variants of the wild-type (wt) estrogen receptor beta (ERbeta-1) coexist in a wide range of tissues. In the human these include, together with others, the expression of several isoforms (ERbeta-2-ERbeta-5) due to alternative splicing of exons encoding the carboxy terminus. In this study, we determined whet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.019
更新日期：2005-08-01 00:00:00
abstract：:Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.013
更新日期：2018-08-01 00:00:00
abstract：:Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.005
更新日期：2015-11-01 00:00:00
abstract：:The causes of vitreous humor (VH) liquefaction remain unclear. Diabetes accelerates this process and other ocular diseases. The weakening of the blood-retina barrier observed with diabetes could enhance the rate of transfer of relatively small molecules such as glucose (Glu) and phospholipids (PLs) from the retina to ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108221
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:Glaucoma is characterized by the neurodegeneration of retinal ganglion cells (RGCs) and the optic nerve. Numerous studies have reported that S100A4 participates in the metastasis of tumor cells and nerve protection. This study was intended to explore the role of S100A4 on RGCs under retinal ischemia-reperfus...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108281
更新日期：2020-12-01 00:00:00
abstract：:The lipid composition of retinal pigment epithelial cells was determined for normal cells which have full phagocytic ability and for a genetic variant with impaired phagocytic function. Retinal pigment epithelial cells from 9-14-day-old congenic strains of normal (RCS-rdy+) and dystrophic (RCS-rdy/rdy) rats were separ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80006-9
更新日期：1986-11-01 00:00:00
abstract：:Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90095-v
更新日期：1991-03-01 00:00:00
abstract：:Recently, a unique distribution, namely a reduction of macular pigment optical density (MPOD) within the central retina with a surrounding ring-like structure of preserved MPOD at about 6 degrees eccentricity was suggested to be a common finding in macular telangiectasia (MacTel) type 2. In order to quantify this redu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.006
更新日期：2009-06-15 00:00:00
abstract：:Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90059-6
更新日期：1989-06-01 00:00:00
abstract：:The purpose of this study was to investigate the effects of soluble VEGF on human choroidal endothelial cell (CEC) transmigration across an RPE monolayer as it relates to choroidal neovascularization in AMD. In coculture assays, ARPE-19 (ARPE) was plated on the undersides of Transwell inserts having 0.4 microm pores. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.021
更新日期：2006-04-01 00:00:00
abstract：:In retinal degenerative disease (RD), the diminished light signal from dying photoreceptors has been considered the sole cause of visual impairment. Recent studies show a 10-fold increase in spontaneous activity in the RD network, challenging this paradigm. This aberrant activity forms a new barrier for the light sign...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.05.013
更新日期：2016-09-01 00:00:00
abstract：:The purpose of this study was to evaluate the composition of ghost vessels and the newly identified intravitreal structures type 1 and 2 (IVS-1 and 2) observed in the rabbit vitreous body. Rabbit eyes (n = 10, 0.5- approximately 36 months of age) were fixed and embedded in plastic. Post-embedding immuno transmission e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0865
更新日期：2000-08-01 00:00:00
abstract：:We examine the immunoreactivity of the non-receptor tyrosine kinase, c-Src kinase and its downstream molecule, signal transducer and activator of transcription 3 (pStat3) in retinoblastoma (RB), and correlation with invasiveness and differentiation. Tumor samples from 40 patients with RB were available for the study. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.011
更新日期：2006-10-01 00:00:00
abstract：:Myofibroblast development and haze generation in the corneal stroma is mediated by cytokines, including transforming growth factor-beta (TGF-beta), and possibly other cytokines. This study examined the effects of stromal PDGF-beta blockade on the development of myofibroblasts in response to -9.0 diopter photorefractiv...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.12.006
更新日期：2009-05-01 00:00:00
abstract：:Loss of vision in severe retinal degenerations often is a result of photoreceptor cell or retinal pigment epithelial cell death or dysfunction. Cell replacement therapy has the potential to restore useful vision for these individuals especially after they have lost most or all of their light-sensing cells in the eye. ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.07.010
更新日期：2014-06-01 00:00:00
abstract：PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108212
更新日期：2020-11-01 00:00:00
