Abstract：
:Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stained positively with dialyzed iron, alcian blue and cationized ferritin, all of which are markers for anionic sulfate or carboxyl groups. The corneal surface was negative for high iron diamine, which specifically stains sulfate groups. These results indicate that the murine ocular surface is rich in carboxyl groups. Treatment with neuraminidase prior to fixation significantly reduced (P less than 0.005) cationic ferritin binding, suggesting that most of the carboxyl groups at the ocular surface are associated with sialic acid residues. The corneal surface also stained positively at the TEM level when a periodic acid-thiocarbohydrazide-silver protein sequence (PA-T-SP) was applied. This result indicated the presence of periodic acid-Schiff (PAS)-positive glycoprotein and glycolipid at the ocular surface.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Wells PA,Hazlett LDdoi
10.1016/0014-4835(84)90111-8subject
Has Abstractpub_date
1984-07-01 00:00:00pages
19-35issue
1eissn
0014-4835issn
1096-0007journal_volume
39pub_type
杂志文章abstract：:To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0429
更新日期：1998-03-01 00:00:00
abstract：:We investigated the pharmacological actions of hydrogen sulfide (H(2)S) using sodium hydrosulfide (NaHS) and sodium sulfide (Na(2)S) as donors on isolated porcine irides in the presence of tone induced by muscarinic receptor stimulation. Furthermore, we also investigated the mechanism of action of H(2)S in this smooth...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.09.011
更新日期：2008-12-01 00:00:00
abstract：:A prior study found that inhibition of nitric oxide synthase with L-NAME causes a large, rapid decrease in IOP in anesthetized rabbits. In this follow-up study we sought to determine if this hypotensive effect was due to decreased aqueous production, possibly caused by ciliary vasoconstriction. Two protocols were perf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1050
更新日期：2001-09-01 00:00:00
abstract：:This study of lens protein composition found that some cytoskeletal proteins were degraded during the earliest stages of cataract formation. Cataract was induced in 13-14 day old rats by a single subcutaneous injection of sodium selenite (19 mumol kg-1). By 24 hr after the injection of selenite, the ratio of insoluble...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0220
更新日期：1997-03-01 00:00:00
abstract：:Research with animal models of Pseudomonas aeruginosa keratitis has shown that use of a topical corticosteroid alone against an established infection can significantly increase the number of colonizing bacteria or worsen clinical disease. Moreover, retrospective analysis has suggested that corticosteroid use in humans...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.10.010
更新日期：2019-02-01 00:00:00
abstract：:Harmonic analysis was used to characterize the rabbit flicker ERG elicited by sinusoidally modulated full-field stimuli under light-adapted conditions. The frequency-response function for fundamental amplitude, derived from Fourier analysis of the ERG waveforms, exhibited two limbs, with an amplitude minimum at approx...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.10.005
更新日期：2010-12-01 00:00:00
abstract：:Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.11.001
更新日期：2018-02-01 00:00:00
abstract：:The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90019-o
更新日期：1992-05-01 00:00:00
abstract：:In order to study acute retinal toxicity of intravitreal triamcinolone acetonide (TA) at high doses in an animal model, thirty New Zealand albino rabbits were injected with intravitreal TA. The animals were divided in five groups: Group 1 received an intravitreal injection of 0.1 mL balanced salt solution; Group 2, 0....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.10.006
更新日期：2007-02-01 00:00:00
abstract：:High-mobility group Box-1 (HMGB1) is known to be released from injured cells and to induce an inflammatory response. Although HMGB1 was reported to mediate ischemia-reperfusion injury of the brain, its role in glutamate excitotoxicity of the retina remains controversial. Here, the authors demonstrated the evidence tha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.003
更新日期：2015-08-01 00:00:00
abstract：:The transmembrane glycoprotein CD44 is a cell adhesion molecule which has recently been localized in a variety of cell types. It mediates cell attachment to extracellular matrix components and also binds to the actin cytoskeleton within the cell. In this study, the presence and distribution of CD44 in the mouse retina...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1026
更新日期：1994-03-01 00:00:00
abstract：:Adenosine, produced from the decomposition of adenosine triphosphate, is believed to provide protective effects during ischemia. On the other hand, adenosine metabolites may serve as precursors for oxygen free radical formation. The time course of formation of adenosine and its purine metabolites was studied during re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0391
更新日期：1997-12-01 00:00:00
abstract：:Macular corneal dystrophy (MCD) is an autosomal recessive disease characterized by abnormal deposition of glycosaminoglycans in corneal stroma, keratocytes, Descemet's membrane and corneal endothelium. According to the presence and distribution of sulfated keratan sulfate (KS)-epitopes in serum and cornea (using mAb 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1080
更新日期：2001-11-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
abstract：:Transforming growth factor β (TGFβ) signaling is one of the most important signaling pathways regulating cell behavior in ocular tissues. Its functions are mainly linked to tissue fibrosis and inflammatory responses in ophthalmology. In epithelial cells, however, the growth inhibitory activity of TGFβ was reported in ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.03.014
更新日期：2019-05-01 00:00:00
abstract：:There is currently no available method to efficiently deliver proteins across the plasma membrane of photoreceptor or retinal pigment epithelium (RPE) cells in vivo. Thus, current clinical application of recombinant proteins in ophthalmology is limited to the use of proteins that perform their biological function extr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.009
更新日期：2016-04-01 00:00:00
abstract：:When 15-day-old developing chick embryos were administered hydrocortisone hemisuccinate sodium (HC:0.25 mumol per egg) the concentration of glucose in both the aqueous humor and the vitreous body began to increase significantly after 3 hr and reached 24.4 +/- 0.3 mM and 22.3 +/- 0.9 mM, respectively, at 48 hr. Thereaf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80050-8
更新日期：1989-10-01 00:00:00
abstract：:Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2009.03.024
更新日期：2009-09-01 00:00:00
abstract：:In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this s...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.06.006
更新日期：2005-01-01 00:00:00
abstract：:Limbal microvascular endothelial cells (L-MVEC) contribute to formation of the corneal-limbal stem cell niche and to neovascularization of diseased and injuries corneas. Nevertheless, despite these important roles in corneal health and disease, few attempts have been made to isolate L-MVEC with the view to studying th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.12.008
更新日期：2015-02-01 00:00:00
abstract：:The C-terminal region of peripherin/rds contains three predicted alpha-helical domains. One of these domains, corresponding to amino acids 311-322, form an amphiphilic alpha-helix previously shown to promote membrane fusion. The present studies were conducted to determine how the additional alpha-helical regions of th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.2013
更新日期：2002-08-01 00:00:00
abstract：:An investigation was undertaken to expand the data base for elemental concentrations within eye tissues of different species. This report provides data on the distribution of calcium, copper, iron and zinc in human, dog, bovine, bird, amphibian and fish ocular tissues. The variation between different eyes of the same ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90138-0
更新日期：1983-12-01 00:00:00
abstract：:Retinoblastoma generally occurs before 5 years of age and often requires enucleation (surgical removal of one eye) of the cancerous eye. We have previously shown using behavioural methods that this disruption in binocular vision during the critical period of visual development results in impaired face perception. In t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.09.013
更新日期：2019-06-01 00:00:00
abstract：:The effects of the instillation of a PGE2-analogue (11-deoxy-13,14-didehydro-16 (S)-methyl PGE2 methylester: FCE 20700) in the conjunctival sac of the rabbit were studied by means of two methods. The former is a clinical study (Dohlman test), the latter is a morphological investigation (semithin sections) on specimens...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80113-6
更新日期：1987-11-01 00:00:00
abstract：:Myopia is one of the leading ocular disorders causing visual impairment worldwide, with the prevalence increasing rapidly. It's a significant global public health concern in 21st century. Myopia, particularly high myopia, often exhibits apparent familial aggregation, and multiple evidences have shown that genetic fact...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107778
更新日期：2019-11-01 00:00:00
abstract：:Antibiotics such as fluoroquinolones (FQLs) are commonly used to treat ocular infections but are also known to cause dermal melanocyte toxicity. The release of dispersed pigments from the iris into the aqueous humor has been considered a possible ocular side effect of the systemic administration of FQLs such as Moxifl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.006
更新日期：2017-09-01 00:00:00
abstract：:Movement of water in the lens, specifically water diffusion, was examined with diffusion imaging and H217O-enhanced proton imaging. Diffusion imaging showed restricted water self-diffusion in the cortex which was not concurrent with the change in proton density; nevertheless it was dependent on the gradient direction ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90076-5
更新日期：1992-01-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:Recent epidemiological evidence in children indicates that time spent outdoors is protective against myopia. Studies in animal models (chick, macaque, tree shrew) have found that light levels (similar to being in the shade outdoors) that are mildly elevated compared to indoor levels, slow form-deprivation myopia and (...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.05.004
更新日期：2013-09-01 00:00:00
abstract：:The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0369
更新日期：1997-11-01 00:00:00
