Effects of a stable analogue of PGE2 (11-deoxy-13,14-didehydro-16 (S)-methylester methyl PGE2: FCE 20700) on the secretory processes of conjunctival goblet cells of rabbit.

abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...

journal_title：Experimental eye research

authors： de Souza BM,Assmann TS,Kliemann LM,Marcon AS,Gross JL,Canani LH,Crispim D

更新日期：2012-01-01 00:00:00

abstract：:Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is cap...

journal_title：Experimental eye research

authors： Meakin SO,Reddan JR,Tsui LC,Breitman ML

更新日期：1989-01-01 00:00:00

abstract：:Myocilin is a 55-57kDa secreted glycoprotein and member of the olfactomedin family, which is mutated in some forms of primary open-angle glaucoma. To assess the effects of elevated amounts of myocilin on aqueous humor outflow dynamics in an in vivo system, transgenic betaB1-crystallin-MYOC mice have been developed tha...

journal_title：Experimental eye research

authors： Paper W,Kroeber M,Heersink S,Stephan DA,Fuchshofer R,Russell P,Tamm ER

更新日期：2008-09-01 00:00:00

abstract：:To trace the path taken by the putative postganglionic secretomotor fibres to the lacrimal gland the contents of the orbital and pterygopalatine fossa were removed whole, cut coronally into slabs and embedded in resin. Thin sections were cut at varying intervals to reconstruct the pathway taken. One group of rami orbi...

journal_title：Experimental eye research

authors： Ruskell GL

更新日期：2004-03-01 00:00:00

abstract：:Laser Doppler flowmetry (LDF) measures the flux of red blood cells in a vascular network embedded in tissue. In the present report, choroidal and ciliary body blood flow was measured continuously using an LDF technique in pentobarbital anesthetized cats. Blood flow measurements were made from the choroid beneath the l...

journal_title：Experimental eye research

authors： Gherezghiher T,Okubo H,Koss MC

更新日期：1991-08-01 00:00:00

abstract：:The relative distribution of acetylcholine (ACh) receptors on the surface of the isolated ocular lens of the rabbit was determined from induced changes in translens short-circuit current (I(SC)) and the translenticular resistance (R(t)) at seven delineated, parallel zones from the anterior to the posterior pole. For t...

journal_title：Experimental eye research

authors： Candia OA,Zamudio AC,Polikoff LA,Alvarez LJ

更新日期：2002-06-01 00:00:00

abstract：:Corneal endothelial wound healing following scrape injury in the rabbit and cat is characterized by cell spreading and maintenance of a normal endothelial phenotype consisting of apically-localized, circumferential microfilament bands and cell border-associated ZO-1, a tight junction protein and marker for endothelial...

journal_title：Experimental eye research

authors： Petroll WM,Barry-Lane PA,Cavanagh HD,Jester JV

更新日期：1997-02-01 00:00:00

abstract：:Choroidal neovascularization (CNV) associated with the 'wet' form of age related macular degeneration (AMD) is one of the most common causes of central vision loss among the elderly. The 'wet' form of AMD is currently treated by intravitreal delivery of anti-VEGF agents. However, intravitreal injections are associated...

journal_title：Experimental eye research

authors： Leaderer D,Cashman SM,Kumar-Singh R

更新日期：2015-11-01 00:00:00

abstract：:Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newl...

journal_title：Experimental eye research

authors： Casaroli Marano RP,Preissner KT,Vilaró S

更新日期：1995-01-01 00:00:00

abstract：:We showed previously that treatment of cultured rabbit lens epithelial cells (LECs) with hyperbaric oxygen (HBO) produced DNA strand-breaks, caused reversible inhibition of protein synthesis and induced the synthesis of a 32 kD protein. In the present work, we employed immunostaining procedures to identify the 32 kD p...

journal_title：Experimental eye research

authors： Padgaonkar VA,Giblin FJ,Fowler K,Leverenz VR,Reddan JR,Dziedzic DC

更新日期：1997-09-01 00:00:00

abstract：:Antibiotics such as fluoroquinolones (FQLs) are commonly used to treat ocular infections but are also known to cause dermal melanocyte toxicity. The release of dispersed pigments from the iris into the aqueous humor has been considered a possible ocular side effect of the systemic administration of FQLs such as Moxifl...

journal_title：Experimental eye research

authors： Mahanty S,Kawali AA,Dakappa SS,Mahendradas P,Kurian M,Kharbanda V,Shetty R,Setty SRG

更新日期：2017-09-01 00:00:00

abstract：:It has been suggested that human iris pigment epithelial (IPE) cells isolated from iridectomized tissue could be used as autologous cells for transplantation into the subretinal space in diseases with dysfunctional retinal pigment epithelium (RPE). RPE cells synthesize a number of cytokines and their receptors which a...

journal_title：Experimental eye research

authors： Kociok N,Heppekausen H,Schraermeyer U,Esser P,Thumann G,Grisanti S,Heimann K

更新日期：1998-08-01 00:00:00

abstract：:Iron accumulation in the retina is associated with the development of age-related macular degeneration (AMD). IV iron is a common method to treat iron deficiency anemia in adults, and its retinal manifestations have not hitherto been identified. To assess whether IV iron formulations can be retina-toxic, we generated ...

journal_title：Experimental eye research

authors： Song D,Kanu LN,Li Y,Kelly KL,Bhuyan RK,Aleman T,Morgan JI,Dunaief JL

更新日期：2016-10-01 00:00:00

abstract：:Exposure to hyposmotic solution causes release of ATP from lens cells via hemichannels. Because hemichannel opening feasibly could swamp the cells with calcium, we carried out studies to measure the magnitude of the increase in cytoplasmic calcium concentration caused by hemichannel opening. In studies on porcine lens...

journal_title：Experimental eye research

authors： Mandal A,Shahidullah M,Delamere NA

更新日期：2015-03-01 00:00:00

abstract：:Keratoconus is a debilitating corneal thinning disease that principally develops in the second and third decades of life. Our group previously developed a novel approach to studying keratoconus, based on the observation that there is a gradient of damage across the keratoconic cone. We identified a number of cellular ...

journal_title：Experimental eye research

authors： Brookes NH,Loh IP,Clover GM,Poole CA,Sherwin T

更新日期：2003-10-01 00:00:00

abstract：:In ocular surface inflammatory diseases, such as dry eye disease, long-term symptom relief requires targeting the inflammation itself rather than treating only the surface-associated dryness with artificial tears. Therefore, we included an anti-inflammatory agent in an unpreserved liposome-based (LP) formulation used ...

journal_title：Experimental eye research

authors： Soriano-Romaní L,Vicario-de-la-Torre M,Crespo-Moral M,López-García A,Herrero-Vanrell R,Molina-Martínez IT,Diebold Y

更新日期：2017-01-01 00:00:00

abstract：:Glucosyltransferase, an enzyme involved in the synthesis of the carbohydrate portion of basement membranes and collagens, and collagen content were determined in the retina of normal and streptozotocin diabetic rats. No significant difference in glucosyltransferase activity was found at 4 weeks of diabetes. However, t...

journal_title：Experimental eye research

authors： Reddi AS

更新日期：1985-09-01 00:00:00

abstract：:Several variants of growth hormone (GH) are found in the retina and vitreous of the chick embryo, where they appear to act as cell survival factors, having neuroprotective effects on retinal ganglion cells (RGCs). Here, we investigate the molecular mechanisms of the anti-apoptotic effect of GH in cultured RGCs. GH tre...

journal_title：Experimental eye research

authors： Sanders EJ,Parker E,Harvey S

更新日期：2006-11-01 00:00:00

abstract：:Experimental autoimmune uveoretinitis (EAU) in mice provides a useful platform to study the pathogenesis and experimental therapeutics of human uveitis. One often used EAU model employs C57BL/6 (B6) mice sensitized with a peptide residue having 1 to 20 amino acids of human interphotoreceptor retinoid binding protein (...

journal_title：Experimental eye research

authors： Tajiri N,Kato T,Satoh M,Iizuka M,Taniguchi M,Kitaichi N,Iwabuchi K

更新日期：2020-12-19 00:00:00

abstract：:The corneal endothelium of man, primates and carnivores has limited regenerative ability. Fibroblast growth factor (FGF) stimulates the proliferation of various mesoderm-derived cells, including corneal endothelial cells, in vitro. In the present work the effect of FGF on the regeneration of injured cat endothelium in...

journal_title：Experimental eye research

authors： Landshman N,Belkin M,Ben-Hanan I,Ben-Chaim O,Assia E,Savion N

更新日期：1987-12-01 00:00:00

abstract：:The P23H-1 rat strain carries a rhodopsin mutation frequently found in retinitis pigmentosa patients. We investigated the progressive degeneration of the inner retina in this strain, focussing on retinal ganglion cells (RGCs) fate. Our data show that photoreceptor death commences in the ventral retina, spreading to th...

journal_title：Experimental eye research

authors： García-Ayuso D,Salinas-Navarro M,Agudo M,Cuenca N,Pinilla I,Vidal-Sanz M,Villegas-Pérez MP

更新日期：2010-12-01 00:00:00

abstract：:Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas of SDS gels (Western blots) were used in a developmental study to detect the presence and localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their BALB/c normal controls. Western blot analysis of...

journal_title：Experimental eye research

authors： Usukura J,Bok D

更新日期：1987-10-01 00:00:00

abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...

journal_title：Experimental eye research

authors： McDowell CM,Tebow HE,Wordinger RJ,Clark AF

更新日期：2013-11-01 00:00:00

abstract：:The objective of this study was to compare the efficiency of various formulations in maximizing the ratio of ocular to systemic absorption of topically applied timolol in the pigmented rabbit. Formulations of various pHs, tonicities, and concentrations of benzalkonium chloride, EDTA, poly(vinyl alcohol), poly(vinylpyr...

journal_title：Experimental eye research

authors： Podder SK,Moy KC,Lee VH

更新日期：1992-05-01 00:00:00

abstract：:The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...

journal_title：Experimental eye research

authors： Winkler J,Wirbelauer C,Frank V,Laqua H

更新日期：2001-03-01 00:00:00

abstract：:Thirty-one trabeculectomy specimens of patients suffering from primary open-angle glaucoma (POAG) who had received no, or only minor, medication prior to surgery were ultrastructurally and quantitatively analysed. Most of the specimens revealed thickened trabeculae, increased amounts of plaque-material deposited withi...

journal_title：Experimental eye research

authors： Rohen JW,Lütjen-Drecoll E,Flügel C,Meyer M,Grierson I

更新日期：1993-06-01 00:00:00

abstract：:Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...

journal_title：Experimental eye research

authors： Wiechmann AF,Font RL,Hollyfield JG

更新日期：1991-03-01 00:00:00

abstract：:The cornea receives the densest sensory innervation of the body, which is exclusively from small-fiber nociceptive (pain-sensing) neurons. These are similar to those in the skin of the legs, the standard location for neurodiagnostic skin biopsies used to diagnose small-fiber peripheral polyneuropathies. Many cancer ch...

journal_title：Experimental eye research

authors： Ferrari G,Nallasamy N,Downs H,Dana R,Oaklander AL

更新日期：2013-08-01 00:00:00

abstract：:Myofibroblasts expressing α-smooth muscle actin (α-SMA) are the key cellular mediator of fibrosis. Fibrovascular epiretinal membranes from patients with proliferative diabetic retinopathy (PDR) are characterized by the accumulation of a large number of myofibroblasts. We explored the hypothesis that proliferating endo...

journal_title：Experimental eye research

authors： Abu El-Asrar AM,De Hertogh G,van den Eynde K,Alam K,Van Raemdonck K,Opdenakker G,Van Damme J,Geboes K,Struyf S

更新日期：2015-03-01 00:00:00

abstract：:Bovine corneal endothelial cells in culture bind internalize and degrade [125I]-trypsin. Binding involves the active site of trypsin and increases as a function of [125I]-trypsin concentration. Saturation is observed at a concentration of 0.5-1.0 micrograms ml-1. The cell surface binding of [125I]-trypsin is specific:...

journal_title：Experimental eye research

authors： Glovinsky J,Savion N

更新日期：1985-05-01 00:00:00