Abstract：
:The corneal endothelium of man, primates and carnivores has limited regenerative ability. Fibroblast growth factor (FGF) stimulates the proliferation of various mesoderm-derived cells, including corneal endothelial cells, in vitro. In the present work the effect of FGF on the regeneration of injured cat endothelium in vivo was studied. Scraping of cat corneal endothelium off Descemet's membrane was carried out by a specially designed instrument. FGF from crude preparations (25 micrograms in 50 microliter saline) or affinity purified FGF (0.5 micrograms in 25 microliter saline) were injected immediately after the scraping of the endothelium, and in both cases significant stimulation of endothelium regeneration was observed. During the first 2 weeks after scraping, the endothelial cell density (ECD) in FGF-treated eyes was higher by a factor of 1.7-2.3 than that in the control eyes. This marked difference decreased with time, but even after 12 weeks the ECD in FGF-treated eyes was higher by a factor of 1.15-1.30 than in the control eyes. Furthermore, FGF improved the polygonal shape of the cells and decreased the corneal thickness. These results clearly demonstrate the efficacy of FGF in inducing the proliferation of cat corneal endothelial cells in vivo and indicate its potential application in clinical practice.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Landshman N,Belkin M,Ben-Hanan I,Ben-Chaim O,Assia E,Savion Ndoi
10.1016/s0014-4835(87)80097-0subject
Has Abstractpub_date
1987-12-01 00:00:00pages
805-11issue
6eissn
0014-4835issn
1096-0007journal_volume
45pub_type
杂志文章abstract：:A lens serine proteinase with trypsin-like specificity has been purified to homogeneity. This is one of two serine proteinases associated with the alpha-crystallin fraction from bovine lens. The purification was accomplished by a combination of isoelectric precipitation, activation to release the proteinase, gel-filtr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90135-5
更新日期：1983-12-01 00:00:00
abstract：:Recent studies indicate a key role of Mitf (microphthalmia-associated transcription factor) in the differentiation of the retinal pigment epithelium (RPE). To explore transdifferentiation processes, we used small interfering RNA (siRNA) targeting Mitf. Transfection of embryonic chick RPE cells with a non-silencing flu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.004
更新日期：2005-07-01 00:00:00
abstract：:The purpose of this study was to evaluate the composition of ghost vessels and the newly identified intravitreal structures type 1 and 2 (IVS-1 and 2) observed in the rabbit vitreous body. Rabbit eyes (n = 10, 0.5- approximately 36 months of age) were fixed and embedded in plastic. Post-embedding immuno transmission e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0865
更新日期：2000-08-01 00:00:00
abstract：:Human Kv3.3/KCNC3 is a Shaw-type potassium channel that has been mapped to chromosome 19q13.3-13.4. Complete mouse and rat Kv3.3 cDNA coding sequences have been published, yet the human Kv3.3 cDNA has remained incomplete for years. We report here for the first time the amino acid sequence for hKv3.3 and the electrophy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0796
更新日期：2000-03-01 00:00:00
abstract：:Exfoliation syndrome (XFS) is an important risk factor for glaucoma (XFG) worldwide. LOXL1 variants are highly associated with XFS in most populations; however, the high frequency of risk alleles in normal individuals and the reversal of risk alleles in different ethnic populations suggest that other factors contribut...
journal_title：Experimental eye research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.exer.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：:Understanding how tissues and organs acquire and maintain an appropriate size and shape remains one of the most challenging areas in developmental biology. The eye lens represents an excellent system to provide insights into regulatory mechanisms because in addition to its relative simplicity in cellular composition (...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.04.009
更新日期：2017-03-01 00:00:00
abstract：:Addition of substance P (10(-7) to 10(-6) M) to rabbit iris sphincter muscle induced: (a) a rapid phosphodiesteratic breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) into 1,2-diacylglycerol (DG), measured as phosphatidic acid, and inositol triphosphate (IP3), measured by anion-exchange chromatography; (b) a r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80089-6
更新日期：1986-08-01 00:00:00
abstract：:Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.01.033
更新日期：2006-09-01 00:00:00
abstract：:The suitability of the rat derived SV-40T immortalized RPE-J cell line for identifying proteome changes associated with RPE differentiation was evaluated by surveying changes in protein expression levels. Rat RPE-J cells were induced to undergo differentiation in culture by growth at the nonpermissive temperature of 4...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1058
更新日期：2001-10-01 00:00:00
abstract：:Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90095-v
更新日期：1991-03-01 00:00:00
abstract：:The R49C mutation of alphaA-crystallin (alphaA-R49C) causes hereditary cataracts in humans; patients in a four-generation Caucasian family were found be heterozygous for this autosomal dominant mutation. We previously generated knock-in mouse models of this mutation and found that by 2 months of age, heterozygous muta...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.02.009
更新日期：2010-06-01 00:00:00
abstract：:Experiments were performed using [1-14C]PGH2 endoperoxide as substrate to assess which, if any, of the PGH2 utilizing enzymes were present in rat lens microsomes. After a 15 min incubation, prostaglandins (PGs) were extracted, separated by thin-layer chromatography and labeled PGs located by autoradiography. The bands...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80010-6
更新日期：1987-02-01 00:00:00
abstract：:This study was performed to further characterize expression and function of the Fas/Fas ligand system in the cornea. Specifically, these experiments examined (1) the effect of genetic inactivation of Fas or Fas ligand genes on keratocyte apoptosis in response to corneal epithelial wounding, (2) whether cultured human ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0371
更新日期：1997-10-01 00:00:00
abstract：:This is a brief overview of multifaceted anatomical, experimental and clinical studies conducted by the author since 1955 on the optic nerve head circulation in health and disease. Conclusions, based on the accumulated information provided by these studies, are summarized. The studies on the pattern of blood supply of...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(05)80121-6
更新日期：1995-09-01 00:00:00
abstract：:Two low-Ca2+-requiring proteases (calpain I) and one high-Ca2+-requiring protease (calpain II) have been separated from the cytosol of rat retina by DEAE-cellulose column chromatography. Calpain I was half-maximally activated at 3 microM free Ca2+ and fully activated at 10 microM free Ca2+. Half-maximal activation of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90098-3
更新日期：1985-07-01 00:00:00
abstract：:Long living animal models of retinitis pigmentosa (RP) can provide important information on the retinal changes that occur at the late stages of photoreceptor degeneration. The rhodopsin Pro347Leu transgenic rabbit (P347L Tg) is a model of RP, and it has been used to analyze the functional and morphological changes in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.026
更新日期：2019-07-01 00:00:00
abstract：:Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.12.004
更新日期：2014-03-01 00:00:00
abstract：:Rhegmatogenous retinal detachment is by far the most common indication for retinal surgery and a major cause of severe vision loss. Increased levels of glutamate found in the vitreous of human patients and persistent remodeling, even after reattachment, suggest substantial neurochemical, functional and anatomical chan...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.02.009
更新日期：2012-04-01 00:00:00
abstract：:The combined effects of adenosine and acetylcholine on the intracellular free-Ca2+ concentration in nonpigmented epithelial cells of the rabbit ciliary body were investigated using fura-2 fluorescence-ratio imaging. Acetylcholine (10 microM) by itself produced a modest increase in [Ca2+]i. Acetylcholine in combination...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0194
更新日期：1997-02-01 00:00:00
abstract：:The sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA) is a key intracellular calcium transporter, which regulates cellular calcium concentration [Ca2+] by transporting Ca2+ ions from the cytosol into the endoplasmic reticulum. SERCA-mediated Ca2+ sequestration controls proper folding of newly synthesized prote...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.007
更新日期：2005-12-01 00:00:00
abstract：:Biochemical alterations in the composition of retinal capillary basement membrane components were investigated in galactosemic rats, an animal model that develops basement membrane lesions comparable to those of diabetic retinopathy. Normotensive Wistar-Kyoto rats fed a 30% galactose diet for 9 months developed signif...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90211-c
更新日期：1990-03-01 00:00:00
abstract：:Extracts of bovine corneal stroma have been shown to activate keratocytes in culture to proliferate. We fractionated stromal extract on a column of Sephacryl S-300 and tested the fractions for mitogenic activity using cell culture and for the presence of IGF-II and its binding protein IGFBP-2 by Western blot. We found...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.12.004
更新日期：2008-03-01 00:00:00
abstract：:A prior study found that inhibition of nitric oxide synthase with L-NAME causes a large, rapid decrease in IOP in anesthetized rabbits. In this follow-up study we sought to determine if this hypotensive effect was due to decreased aqueous production, possibly caused by ciliary vasoconstriction. Two protocols were perf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1050
更新日期：2001-09-01 00:00:00
abstract：:Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotec...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.022
更新日期：2011-11-01 00:00:00
abstract：:Excised bovine and rhesus monkey vitreous samples were subjected to different hydrostatic pressures (0.03, 1 and 2 atm) for 12 hr. At the end of the experiment the samples were frozen in a -70 degrees C dry ice-acetone bath and thereafter maintained in frozen conditions. Differential scanning calorimetry (DSC) was use...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1021
更新日期：2001-07-01 00:00:00
abstract：:The transport kinetics across the plasma-ocular barriers of labeled molecules including urea, D-glucose, 3-0-methyl-D-glucose, L-glucose, and sucrose were studied in adult RCS rats and compared to control albino Sprague-Dawleys (SD). Results indicate that substances that passively cross the plasma-aqueous and plasma-v...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90156-2
更新日期：1983-09-01 00:00:00
abstract：:The purpose of this study was to investigate the roles of endothelium-derived factors in the retinal arteriolar responses to acute severe elevation in systemic blood pressure (BP) in cats. Acute elevation of mean arterial BP by 60% for 5 min was achieved by inflating a balloon-tipped catheter in the descending aorta. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.08.007
更新日期：2012-10-01 00:00:00
abstract：:This study describes the phosphatic metabolite contribution of the porcine corneal stroma [by phosphorus-31 nuclear magnetic resonance (P-31 NMR)] and the corneal epithelium (by difference calculation) to the total phosphatic metabolic profile of the intact (i.e. whole, undissected) cornea. In order to provide such a ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90147-2
更新日期：1985-03-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:Peroxisomes were localized in the head of wild-type and mutant strains of Drosophila melanogaster by use of a cytochemical method for the demonstration of D-amino acid oxidase activity. With similar techniques we had found previously that vertebrate photoreceptors have few, if any, bodies with cytochemically demonstra...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90092-9
更新日期：1990-11-01 00:00:00
