Abstract:
:The sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA) is a key intracellular calcium transporter, which regulates cellular calcium concentration [Ca2+] by transporting Ca2+ ions from the cytosol into the endoplasmic reticulum. SERCA-mediated Ca2+ sequestration controls proper folding of newly synthesized proteins within the ER as well as the timing and spatial patterning of depolarization-evoked Ca2+ responses in the cytoplasm. To understand the spatial and temporal properties of Ca2+ homeostasis in retinal neurons better, I studied expression and distribution of all three SERCA isoforms in the mouse retina using isoform-specific antibodies. No immunostaining was observed with the SERCA1 antibody. SERCA2 was expressed in photoreceptor inner segments, amacrine and ganglion cells of the mouse retina. Similar SERCA2 localization was observed in adult rat, macaque and ground squirrel retinas. Analysis of distribution of SERCA2 immunofluorescence in the developing mouse retina revealed prominent SERCA2 signals throughout postnatal development. The N89 antibodys used to identify the SERCA3 isoforms labelled cone outer segments, inner segments of photoreceptors and cell processes in the inner nuclear layer of the mouse retina. These results imply that the SERCA2 isoform controls Ca2+ sequestration into the endoplasmic reticulum in most classes of retinal neuron. A potential role for SERCA3 in cone function is suggested.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Krizaj Ddoi
10.1016/j.exer.2005.04.007keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
690-9issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(05)00121-1journal_volume
81pub_type
杂志文章abstract::Lens development and transparency have been hypothesized to depend on intercellular gap junction channels, consisting of alpha3 (Cx46) and alpha8 (Cx50) connexin subunits, to transport metabolites, secondary messages and ions between lens cells. To evaluate this hypothesis, we have generated alpha3(-/-) alpha8(-/-) do...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.03.013
更新日期:2006-09-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1006/exer.2000.0918
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journal_title:Experimental eye research
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pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80026-5
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2014.05.010
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doi:10.1016/0014-4835(85)90077-6
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pub_type: 杂志文章
doi:10.1016/0014-4835(92)90202-4
更新日期:1992-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80010-6
更新日期:1987-02-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2009.03.020
更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2007.09.013
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journal_title:Experimental eye research
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doi:10.1006/exer.1996.0211
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2015.01.008
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journal_title:Experimental eye research
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