An acute intraocular pressure challenge to assess retinal ganglion cell injury and recovery in the mouse.

abstract：:Omental cells (OCs) are shown to help wound healing. The purpose of this study is to investigate if OCs improve cornea repair after alkali injury by subconjunctival injection of activated OCs in rats. Forty eight hours after limbal corneal alkali injury, fresh isolated OCs were injected subconjunctivally into the reci...

journal_title：Experimental eye research

authors： Bu P,Vin AP,Sethupathi P,Ambrecht LA,Zhai Y,Nikolic N,Qiao L,Bouchard CS

更新日期：2014-04-01 00:00:00

abstract：:The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those aft...

journal_title：Experimental eye research

authors： Yabana T,Hooper ML,Farrell SR,Chauhan BC

更新日期：2021-01-01 00:00:00

abstract：:The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...

journal_title：Experimental eye research

authors： Funk R,Rohen JW

更新日期：1985-02-01 00:00:00

abstract：:Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...

journal_title：Experimental eye research

authors： Chen Y,Luo X,Liu S,Shen Y

更新日期：2018-02-01 00:00:00

abstract：:Alpha B-crystallin, a major lens protein, was induced in primary cultures of dog lens epithelial cells and glomerular endothelial cells when they were grown under conditions of hypertonic stress. With Western blot analysis using a specific alpha B-crystallin antibody, we observed a significant increase in the concentr...

journal_title：Experimental eye research

authors： Dasgupta S,Hohman TC,Carper D

更新日期：1992-03-01 00:00:00

abstract：:To investigate the nature of the 140 kDa glycoprotein in the trabecular meshwork, polypeptides were extracted with either urea/sodium dodecyl sulfate (SDS)/beta-2-mercaptoethanol (BME) or guanidine hydrochloride followed by pepsin digestion. After electrophoresis and immunoblotting with anti-type-VI-collagen antibodie...

journal_title：Experimental eye research

authors： Tripathi BJ,Hansen M,Li J,Tripathi RC

更新日期：1994-02-01 00:00:00

abstract：:The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...

journal_title：Experimental eye research

authors： Usui T,Kubo Y,Akanuma S,Hosoya K

更新日期：2013-08-01 00:00:00

abstract：:The distribution of collagen degradation and synthesis (turnover) between the central and peripheral areas of clear corneal allografts was determined, following transplantation of 6-mm [3H]proline-labelled corneal buttons from extensively pre-labelled, young rabbits into 13 nonradioactive mature rabbits. Control, cont...

journal_title：Experimental eye research

authors： Lass JH,Baker DS,Klein LR

更新日期：1987-07-01 00:00:00

abstract：:Recurrences of herpetic stromal keratitis are believed to be initiated by reactivation of herpes simplex virus infection, probably in the trigeminal ganglion. Genetic features of the virus and the host as well as the immune status of the host influence the outcome of infection. Following infection on the snout with HS...

journal_title：Experimental eye research

authors： Jager MJ,Bradley D,Atherton S,Streilein JW

更新日期：1992-06-01 00:00:00

abstract：:Previous studies have suggested that a mannose receptor mediates the phagocytic uptake of effete rod outer segments by retinal pigment epithelial cells. In the present study, the effect of adding a soluble ligand for the mannose receptor, horseradish peroxidase, was examined. Cultured retinal pigment epithelial cells ...

journal_title：Experimental eye research

authors： Lutz DA,Guo Y,McLaughlin BJ

更新日期：1995-10-01 00:00:00

abstract：:Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvast...

journal_title：Experimental eye research

authors： Wang Y,Xu SL,Wu YZ,Zhao MS,Xu WJ,Yang HY,Li YX

更新日期：2013-08-01 00:00:00

abstract：:Retinoblastoma generally occurs before 5 years of age and often requires enucleation (surgical removal of one eye) of the cancerous eye. We have previously shown using behavioural methods that this disruption in binocular vision during the critical period of visual development results in impaired face perception. In t...

journal_title：Experimental eye research

authors： Kelly KR,Gallie BL,Steeves JKE

更新日期：2019-06-01 00:00:00

abstract：:Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newl...

journal_title：Experimental eye research

authors： Casaroli Marano RP,Preissner KT,Vilaró S

更新日期：1995-01-01 00:00:00

abstract：:Diabetic retinopathy is a complication of diabetes and a leading cause of vision loss among working-age adults. To assess whether the Wistar rat with Streptozotocin (STZ)-induced diabetes is a suitable animal model of human proliferative diabetic retinopathy we evaluated the vascular changes to assess the diabetic ret...

journal_title：Experimental eye research

authors： Naderi A,Zahed R,Aghajanpour L,Amoli FA,Lashay A

更新日期：2019-07-01 00:00:00

abstract：:The use of murine 3T3 feeder cells needs to be avoided when fabricating corneal epithelial cell sheets for use in treating ocular surface diseases. However, the expression level of the epithelial stem/progenitor cell marker, p63, is down-regulated in feeder-free culture systems. In this study, in order to fabricate co...

journal_title：Experimental eye research

authors： Nakajima R,Takeda S

更新日期：2014-01-01 00:00:00

abstract：:Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...

journal_title：Experimental eye research

authors： Zhang H,Duncan G,Wang L,Liu P,Cui H,Reddan JR,Yang BF,Wormstone IM

更新日期：2007-12-01 00:00:00

abstract：:Simultaneous non-invasive visualization of blood vessels and nerves in patients can be obtained in the eye. The retinal vasculature is a target of many retinopathies. Inflammation, readily manifest by leukocyte adhesion to the endothelial lining, is a key pathophysiological mechanism of many retinopathies, making it a...

journal_title：Experimental eye research

authors： Cahoon JM,Olson PR,Nielson S,Miya TR,Bankhead P,McGeown JG,Curtis TM,Ambati BK

更新日期：2014-03-01 00:00:00

abstract：:Tenon's fibroblast (TF) bleb encapsulation is a common cause of filtering surgery failure. Various pharmacologic agents have been used in an attempt to inhibit this response. The effects of three angiogenesis inhibitors were studied. AGM-1470, a fumagillin analog and hydrocortisone 21-phosphate (HC21P) complexed with ...

journal_title：Experimental eye research

authors： Wong J,Wang N,Miller JW,Schuman JS

更新日期：1994-04-01 00:00:00

abstract：:We sought to characterize the ocular pharmacology, tolerability and intraocular pressure (IOP)-lowering efficacy of FR-190997, a non-peptidic bradykinin (BK) B2-receptor agonist. FR-190997 possessed a relatively high receptor binding affinity (Ki = 27 nM) and a high in vitro potency (EC50 = 18.3 ± 4.4 nM) for inositol...

journal_title：Experimental eye research

authors： Sharif NA,Li L,Katoli P,Xu S,Veltman J,Li B,Scott D,Wax M,Gallar J,Acosta C,Belmonte C

更新日期：2014-11-01 00:00:00

abstract：:An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation. Using FV...

journal_title：Experimental eye research

authors： Ghoghawala SY,Mannis MJ,Murphy CJ,Rosenblatt MI,Isseroff RR

更新日期：2007-06-01 00:00:00

abstract：:The transcription factor Prox1 acts in rodent retinogenesis, at least in promoting cell cycle withdrawal and horizontal cell production. In the mature retina, this protein is detected at the inner nuclear layer of all vertebrate groups. We have made a neurochemical characterisation of Prox1(+) cell types in two differ...

journal_title：Experimental eye research

authors： Cid E,Santos-Ledo A,Parrilla-Monge M,Lillo C,Arévalo R,Lara JM,Aijón J,Velasco A

更新日期：2010-02-01 00:00:00

abstract：:Cysteine cathepsins are a family of proteases involved in intracellular protein turnover and extracellular matrix degradation. Cathepsin B (Ctsb) and cathepsin Z (Ctsz) promote tumorigenesis and Ctsb is a known modulator of tumor angiogenesis. We therefore investigated the angiomodulatory function of these cathepsins ...

journal_title：Experimental eye research

authors： Bühler A,Berger S,Bengsch F,Martin G,Han H,Vierkotten S,Pielen A,Boehringer D,Schlunck G,Fauser S,Agostini HT,Reinheckel T,Stahl A

更新日期：2013-10-01 00:00:00

abstract：:In a variety of tissues including gastrointestinal mucosa, rebamipide (REB) provides cytoprotection, prevents inflammation, and promotes wound healing. Clinically, REB ophthalmic dispersions are used to treat diabetic keratopathy. In this study, we investigated the optimal particle size of REB to promote corneal wound...

journal_title：Experimental eye research

authors： Nagai N,Ito Y,Okamoto N,Shimomura Y

更新日期：2016-10-01 00:00:00

abstract：:Measurement of intraocular pressure (IOP) is an essential tool in monitoring glaucoma. Single IOP assessments during clinical routine examinations represent punctual values and are not able to identify IOP fluctuations and spikes. Telemetric IOP measurements are able to monitor IOP during the day and night, and are lo...

journal_title：Experimental eye research

authors： Mariacher S,Ebner M,Hurst J,Szurman P,Januschowski K

更新日期：2018-01-01 00:00:00

abstract：:Mitochondrial components are of great importance for the maintenance of lens transparency. In our previous work, we confirmed that carbon monoxide (CO) can protect human and rabbit lens epithelial cells (LECs) from hydrogen peroxide (H2O2)-mediated apoptosis, while the mechanism remains undefined. Because CO can bind ...

journal_title：Experimental eye research

authors： Huang Y,Ye Z,Ma T,Li H,Zhao Y,Chen W,Wang Y,Yan X,Gao Y,Li Z

更新日期：2018-04-01 00:00:00

abstract：BACKGROUND:Absence of collagen VII causes blistering of the skin, eyes and many other tissues. This disease is termed dystrophic epidermolysis bullosa (DEB). Corneal fibrosis occurs in up to 41% and vision loss in up to 64% of patients. Standard treatments are supportive and there is no cure. The hypomorphic mouse mode...

journal_title：Experimental eye research

authors： Chen VM,Shelke R,Nyström A,Laver N,Sampson JF,Zhiyi C,Bhat N,Panjwani N

更新日期：2018-10-01 00:00:00

abstract：:Purpose of the study was to develop and assess a novel controlled drug delivery system of latanoprost acid (LA). Poly(lactide)/Monomethoxy-poly(ethyleneglycol) (PLA-PEG) nanoparticles (NPs) were prepared using an emulsification-solvent evaporation technique. NPs were characterized in vitro according to their size, ζ-p...

journal_title：Experimental eye research

authors： Giarmoukakis A,Labiris G,Sideroudi H,Tsimali Z,Koutsospyrou N,Avgoustakis K,Kozobolis V

更新日期：2013-07-01 00:00:00

abstract：:Macular corneal dystrophy (MCD) is an autosomal recessive disease characterized by abnormal deposition of glycosaminoglycans in corneal stroma, keratocytes, Descemet's membrane and corneal endothelium. According to the presence and distribution of sulfated keratan sulfate (KS)-epitopes in serum and cornea (using mAb 5...

journal_title：Experimental eye research

authors： Cursiefen C,Hofmann-Rummelt C,Schlötzer-Schrehardt U,Fischer DC,Haubeck HD,Küchle M,Naumann GO

更新日期：2001-11-01 00:00:00

abstract：:Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...

journal_title：Experimental eye research

authors： Islam MR,Atoji Y

更新日期：2009-09-01 00:00:00

abstract：:Isobutylmethylxanthine (IBMX), a phosphodiesterase/adenosine receptor inhibitor, was combined with norepinephrine (nE), epinephrine (Epi) and isoproterenol, respectively, to evaluate their effect on intraocular pressure (IOP). Application of topical IBMX alone had no measurable effect on IOP. When IBMX was combined wi...

journal_title：Experimental eye research

authors： Hoyng PF,Groeneboer MC,Busch MJ

更新日期：1991-05-01 00:00:00