Abstract：
:Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (L-isoproterenol greater than or equal to L-epinephrine greater than L-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF1 alpha). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Friedman Z,Hackett SF,Campochiaro PAdoi
10.1016/s0014-4835(87)80158-6subject
Has Abstractpub_date
1987-04-01 00:00:00pages
471-9issue
4eissn
0014-4835issn
1096-0007journal_volume
44pub_type
杂志文章abstract：:Exposure to sulfur mustard (SM) may result in severe ocular injuries. While some of the eyes show a clinical resolution of the injury (defined as clinically non-impaired), part of the eyes develop irreversible late ocular pathologies (defined as clinically impaired) that may lead to corneal blindness. Understanding th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.011
更新日期：2019-07-01 00:00:00
abstract：:Vision loss due to various forms of outer retinal degeneration remains a major problem in clinical ophthalmology. Most retinal degenerations are precipitated by genetic mutations affecting the retinal pigment epithelium and sensory retina, but it is becoming increasingly evident that resultant metabolic changes within...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2005.01.018
更新日期：2005-06-01 00:00:00
abstract：:In the neural retina, glial cells control formation of ionic gradients by mediating transmembrane water fluxes through aquaporin (AQP) water channels. Retinal content and immunolocalization of two water channels, AQP1 and AQP4, in the diabetic rat retinas during high-salt loading were examined in this study. Diabetes ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.06.020
更新日期：2009-11-01 00:00:00
abstract：:Mice carrying the rd mutation are affected with an autosomal recessive disease characterized by the total degeneration of retinal photoreceptor cells, which begins at postnatal day 8 and reaches completion by 3 wk of life. Biochemical studies have led to the proposal that a lesion in cGMP metabolism may be the cause o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80079-x
更新日期：1989-02-01 00:00:00
abstract：:Biochemical studies of acidic lens protein degrading activity in the bovine ciliary body were performed. The activity showed two peaks, Fractions A and B, on Sephadex G-75 column chromatography. Fraction A contained cathepsin D and thiol proteinase activities and was inhibited by pepstatin and leupeptin. Fraction B co...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90150-2
更新日期：1985-03-01 00:00:00
abstract：:A number of deep-sea fish have bright yellow lenses whose coloration is attributable to a variety of largely unidentified short-wave absorbing pigments. Here the pigment of the deep-sea fish Stylephorus chordatus has been isolated and identified by NMR and mass spectroscopy as kynurenine; a pigment also found in the h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90091-6
更新日期：1992-07-01 00:00:00
abstract：:Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.013
更新日期：2018-08-01 00:00:00
abstract：:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increase...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
abstract：:Epidermal growth factor (EGF) previously has been shown to stimulate short-term survival in vitro of cells derived from the native amphibian retinal pigment epithelium (RPE). In the present experiments, we have examined intracellular signaling pathways responsible for mediating these survival-specific growth factor ef...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.02.017
更新日期：2004-07-01 00:00:00
abstract：:Our previous gene expression analysis suggested that conjunctival epithelial cells of Sjögren's syndrome (SS) are inclined to hyper-proliferation and keratinisation status. The goal of this study is to elucidate whether such pathological situations really exist in the conjunctival epithelium of SS. Also, involvement o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.07.009
更新日期：2006-03-01 00:00:00
abstract：:The purpose of this study was to determine whether trans-scleral pressure affects scleral solute permeability by altering scleral thickness or hydration, and to investigate the sustained release delivery of dexamethasone. Scleral sections from donor human globes were mounted for in vitro flux studies. Scleral thicknes...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00211-2
更新日期：2004-03-01 00:00:00
abstract：:The purpose of this study was to compare younger and older mice after chronic intraocular pressure (IOP) elevation lasting up to 4 days with respect to mitochondrial density, structure, and movement, as well as axonal integrity, in an ex vivo explant model. We studied 2 transgenic mouse strains, both on a C57BL/6J bac...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.001
更新日期：2018-07-01 00:00:00
abstract：:Although a range of changes in anterior segment structures have been documented to occur during accommodation, the quantification of changes in the structure of the anterior sclera during the accommodation process in human subjects has yet to be examined. This study therefore aimed to investigate the presence of short...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.07.023
更新日期：2018-12-01 00:00:00
abstract：:Photoreceptor degeneration in human photoreceptor dystrophies and in the relevant animal models has been thought to be executed by one common mechanism -- caspase-mediated apoptosis. However, recent experiments have challenged this concept. In previous experiments, analyzing gene expression in the degenerating rd/rd m...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.01.014
更新日期：2006-08-01 00:00:00
abstract：:The purpose of this study was to investigate the influence of molecular shape, conformability, net surface charge and tissue interaction on transscleral diffusion. Unfixed, porcine sclera was clamped in an Ussing chamber. Fluorophore-labelled neutral albumin, neutral dextran, or neutral ficoll were placed in one hemi-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.07.007
更新日期：2012-09-01 00:00:00
abstract：:The aim of this study was to investigate the developmental potential of embryonic stem (ES) cell-derived eye-like structures as a replacement cell therapy model for retinas with N-methyl-d-aspartate (NMDA)-induced damage. For this purpose mouse ES cells were induced to differentiate into eye-like structures in vitro f...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.01.007
更新日期：2007-05-01 00:00:00
abstract：:Staphylococcus is a leading cause of microbial keratitis, characterized by destruction of the cornea by bacterial exoproteins and host-associated factors. The aim of this study was to compare extracellular and cell-associated proteins produced by two different isolates of S. aureus, a virulent clinical isolate (Staph ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.08.016
更新日期：2016-10-01 00:00:00
abstract：:By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80009-0
更新日期：1995-06-01 00:00:00
abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.exer.2010.03.008. The duplicate article has therefore been withdrawn. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.07.012
更新日期：2011-07-29 00:00:00
abstract：:Diabetic retinopathy (DR) is the most common cause of blindness in the working age population. Early events of DR are accompanied by neurodegeneration of the inner retina resulting in ganglion cell loss. These findings together with reduced retinal thickness are observed within the first weeks of experimental DR. Besi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.013
更新日期：2018-09-01 00:00:00
abstract：:This review offers a contemporary history of the renin-angiotensin (RAS) and kallikrein-kinin (KKS) systems with emphasis on how these complex systems affect the eye. It describes the types of communication (cross-talk) between the two systems and evaluates their potential role in the development of diabetic retinopat...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.05.007
更新日期：2018-01-01 00:00:00
abstract：:The purpose of present study is to dissect the role of PI3K/AKT signaling in the anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on rat retinal ganglion cells (RGCs) after optic nerve (ON) crush. The ONs of seventy-two adult male Wistar rats were crushed by a standardized method. Control ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.01.004
更新日期：2010-05-01 00:00:00
abstract：:Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107860
更新日期：2019-12-01 00:00:00
abstract：:The localization of [35S]-sulfate and [3H]-glucosamine in the hamster non-pigmented ciliary epithelium was studied by light and electron microscopic autoradiography. The radioactivity was concentrated in the Golgi complex of the non-pigmented ciliary epithelium 10 mins after injections of [35S]-sulfate or [3H]-glucosa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90009-x
更新日期：1983-02-01 00:00:00
abstract：:By using immunohistochemical techniques, we demonstrated that HLA class I (A, B and C) and HLA class II (DR), the major histocompatibility antigens in man, are expressed constitutively by cells of the trabecular meshwork/Schlemm's canal system and corneal endothelium, as well as by the conjunctival epithelium, Langerh...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90023-n
更新日期：1990-09-01 00:00:00
abstract：:The rapid increase in popularity of extracapsular cataract extraction may predispose the eye to postoperative bacterial infections by introducing viable organisms through the additional instrumentation and irrigation necessary for the extracapsular technique. Lens protein released into the aqueous humor of the anterio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80034-x
更新日期：1989-11-01 00:00:00
abstract：:Previous studies have shown that corneal endothelial cells contain mRNA and protein of various growth factors. However, the role of these endogenous growth factors in corneal endothelial wound healing is not fully elucidated. In the present study, we investigated the role of endogenous factors and several growth facto...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0918
更新日期：2000-12-01 00:00:00
abstract：:Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90048-1
更新日期：1989-09-01 00:00:00
abstract：:Isobutylmethylxanthine (IBMX), a phosphodiesterase/adenosine receptor inhibitor, was combined with norepinephrine (nE), epinephrine (Epi) and isoproterenol, respectively, to evaluate their effect on intraocular pressure (IOP). Application of topical IBMX alone had no measurable effect on IOP. When IBMX was combined wi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90051-f
更新日期：1991-05-01 00:00:00
abstract：:Retinal ischemia/reperfusion (IR) injury causes profound tissue damage, especially retinal ganglion cell death. The aims of the study were twofold: (1) to investigate the benefits of epigallocatechin-3-gallate (EGCG), the major catechin found in tea, after IR challenge, and (2) to elucidate the mechanism of EGCG inhib...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.01.008
更新日期：2008-04-01 00:00:00
