Abstract：
:The aim of this study was to investigate the developmental potential of embryonic stem (ES) cell-derived eye-like structures as a replacement cell therapy model for retinas with N-methyl-d-aspartate (NMDA)-induced damage. For this purpose mouse ES cells were induced to differentiate into eye-like structures in vitro for 10 days and co-cultured with adult mouse retina treated with or without NMDA treatment. NMDA induces excitotoxic neuronal cell death in the inner neural retina, specifically within the ganglion cell layer. After 10 days of co-culture, the specimens were fixed, embedded in paraffin wax and analyzed by immunohistochemistry. Transplanted eye-like structures differentiate into Tuj1-positive neurons when co-cultured with an adult mouse retina and the cells then migrate into the ganglion cell layer. When co-cultured with an NMDA-treated retina, most of the cells migrating into the ganglion cell layer express the ganglion cell-specific markers Hu and Brn3b. Murine ES cell-derived eye-like structures contain cell populations that can differentiate into ganglion-like cells in the host ganglion cell layer in vitro. Moreover, their contribution to the ganglion cell layer was more prominent when ganglion cell specific damage was induced by NMDA administration. These findings suggest that cells prepared from the eye-like structures generated from ES cells may be useful for cell replacement therapy and may also serve as a model system for such therapies.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Aoki H,Hara A,Niwa M,Motohashi T,Suzuki T,Kunisada Tdoi
10.1016/j.exer.2007.01.007subject
Has Abstractpub_date
2007-05-01 00:00:00pages
868-75issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(07)00029-2journal_volume
84pub_type
杂志文章abstract：:The purpose of this study was to investigate the roles of endothelium-derived factors in the retinal arteriolar responses to acute severe elevation in systemic blood pressure (BP) in cats. Acute elevation of mean arterial BP by 60% for 5 min was achieved by inflating a balloon-tipped catheter in the descending aorta. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.08.007
更新日期：2012-10-01 00:00:00
abstract：:Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (L-isoproterenol greater than or equal to L-epinephrine greater than L-norepinephrine) and some prostagla...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80158-6
更新日期：1987-04-01 00:00:00
abstract：:A shift or displacement of the retinal blood vessels (RBVs) with neuroretinal rim thinning indicates the progression of glaucomatous optic neuropathy. In chronic open angle glaucoma, individuals with RBV positional shifts exhibit more rapid visual field loss than those without RBV shifts. The retinal vessels reportedl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.012
更新日期：2017-09-01 00:00:00
abstract：:Hypertension is an independent risk factor for diabetic retinopathy, yet anti-hypertensive medications such as blockade of angiotensin II do not completely protect against vision-threatening vascular disease. We hypothesized that the potent vasoactive factor, endothelin (ET), is up-regulated in diabetic retinopathy an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.06.025
更新日期：2018-11-01 00:00:00
abstract：:We designed an experiment to study the role of light history and polyunsaturated fatty acids (PUFA) on the susceptibility of the albino rat retina to light damage. Albino rats were born to dams that had been kept in either 1 lx or 250 lx cyclic light for 0-4 days prior to delivery and fed one of three diets containing...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80112-5
更新日期：1995-03-01 00:00:00
abstract：:Diabetic retinopathy (DR) is the most common cause of blindness in the working age population. Early events of DR are accompanied by neurodegeneration of the inner retina resulting in ganglion cell loss. These findings together with reduced retinal thickness are observed within the first weeks of experimental DR. Besi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.013
更新日期：2018-09-01 00:00:00
abstract：:Recently, a unique distribution, namely a reduction of macular pigment optical density (MPOD) within the central retina with a surrounding ring-like structure of preserved MPOD at about 6 degrees eccentricity was suggested to be a common finding in macular telangiectasia (MacTel) type 2. In order to quantify this redu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.006
更新日期：2009-06-15 00:00:00
abstract：:The effect of angiotensin II was studied in vitro on ring segments of bovine retinal resistance arteries (i.d. 126-271 microns) and posterior ciliary arteries (i.d. 207-1153 microns). Although the retinal resistance arteries were responsive to 5-hydroxytryptamine, prostaglandin F2 alpha, and changes in extracellular K...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90034-r
更新日期：1990-05-01 00:00:00
abstract：:An experimental study was conducted on eight litters of newborn rats to evaluate the effects of supplemental oxygen administration on the retinal vasculature. The animals and their mothers were kept inside a pressure chamber and treated for the first 5 days of life. On the sixth day, they were removed and kept for fiv...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80179-3
更新日期：1987-03-01 00:00:00
abstract：:Intraocular pressure (IOP) is the most critical risk factor for primary open angle glaucoma (POAG). In most cases of POAG, IOP is increased because of an abnormally high aqueous humor outflow resistance in the juxtacanalicular region of the trabecular meshwork. A distinct structural change in the trabecular meshwork o...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2009.01.005
更新日期：2009-04-01 00:00:00
abstract：:Analysis of UV filter levels in 48 cataractous human lenses was performed with the use of HPLC. A new chromophore with the absorption maximum at 410nm and molecular mass of 369Da was detected and assigned as deaminated 3-hydroxykynurenine O-beta-D-glucoside (3OHCKAG). Cataractous lenses are characterized by the wide r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.03.013
更新日期：2008-06-01 00:00:00
abstract：:The state of water and water fractions in normal and senile cataractous eye lenses was studied by the NMR method. Combining NMR with vacuum dehydration provided additional information on multifractional samples. A new mathematical procedure is presented which separates the characteristic parameters of the different fr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90104-5
更新日期：1983-05-01 00:00:00
abstract：:Myofibroblasts expressing α-smooth muscle actin (α-SMA) are the key cellular mediator of fibrosis. Fibrovascular epiretinal membranes from patients with proliferative diabetic retinopathy (PDR) are characterized by the accumulation of a large number of myofibroblasts. We explored the hypothesis that proliferating endo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.023
更新日期：2015-03-01 00:00:00
abstract：:Inadequate cross-linking between collagen lamellae is a characteristic feature of keratoconus corneas. The formation of covalent bonds between collagen and elastin fibrils, which maintain the biomechanical properties of the cornea, is mediated by the cuproenzyme lysyl oxidase and four lysyl oxidase-like enzymes. The a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.09.005
更新日期：2012-11-01 00:00:00
abstract：:The corneal endothelium of man, primates and carnivores has limited regenerative ability. Fibroblast growth factor (FGF) stimulates the proliferation of various mesoderm-derived cells, including corneal endothelial cells, in vitro. In the present work the effect of FGF on the regeneration of injured cat endothelium in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80097-0
更新日期：1987-12-01 00:00:00
abstract：:This study was performed to determine whether cells in the posterior stroma undergo apoptosis in response to endothelial cell injury and to determine whether basement membrane component nidogen-1 was present in the cornea. New Zealand White rabbits had an olive tip cannula inserted into the anterior chamber to mechani...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.03.025
更新日期：2018-07-01 00:00:00
abstract：:This study was undertaken to develop a feline model of corticosteroid-induced ocular hypertension. In the first experiment, eight cats were selected whose intraocular pressure (17 +/- 0.4 mmHg) was consistently below the mean baseline intraocular pressure of our colony (24 +/- 0.5) during the preceding 2 months. Unila...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80210-6
更新日期：1992-02-01 00:00:00
abstract：:Macular corneal dystrophy (MCD) is an autosomal recessive disease characterized by abnormal deposition of glycosaminoglycans in corneal stroma, keratocytes, Descemet's membrane and corneal endothelium. According to the presence and distribution of sulfated keratan sulfate (KS)-epitopes in serum and cornea (using mAb 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1080
更新日期：2001-11-01 00:00:00
abstract：:The lysosomal proteases cathepsin B and dipeptidyl peptidase II (DPP II) were identified biochemically in the supernatant fraction of rat and guinea-pig lens homogenates, using as substrates CBZ-Arg-Arg-4-methylcoumarin-7-amide (AMC) (5 microM, pH 6.0, 37 degrees C for cathepsin B, and Lys-Ala-AMC or Lys-Pro-AMC (5 mi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80038-6
更新日期：1987-06-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(95)80001-8
更新日期：1995-02-01 00:00:00
abstract：:Anthocyanins (ACs) are phenolic compounds that are distributed widely in fruits and vegetables. Although consumption of these compounds has been shown to improve visual function, the distribution of ACs in ocular tissue has not been examined in detail. The aim of this study was therefore to evaluate the ocular distrib...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.12.019
更新日期：2006-08-01 00:00:00
abstract：:Anti-Vascular Endothelial Growth Factors (Anti-VEGF) agents have received recent interest as potential anti-fibrotic agents for their concurrent use with trabeculectomy. Preliminary cohort studies have revealed improved bleb morphology following trabeculectomy augmented with ranibizumab. The effects of this humanized ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.08.005
更新日期：2014-10-01 00:00:00
abstract：:Albino mice exposed to 1 X 10(-4) W cm-2 of UV radiation in the range 290-400 nm for 12 hr per day developed anterior lens opacities within 1-2 months after onset of the exposure. Histological changes demonstrated marked derangement of the lens epithelium. When the radiation was filtered with glass, eliminating radiat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80011-7
更新日期：1985-10-01 00:00:00
abstract：:Ocular rigidity (OR) is thought to play a role in the pathogenesis of glaucoma, but the lack of reliable non-invasive measurements has been a major technical challenge. We recently developed a clinical method using optical coherence tomography time-lapse imaging and automated choroidal segmentation to measure the puls...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107831
更新日期：2020-01-01 00:00:00
abstract：:The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80042-3
更新日期：1988-06-01 00:00:00
abstract：:Metabolic disturbances in diabetes mellitus include changes in the type and concentration of lipids in the blood plasma which may contribute to the development of diabetic retinopathy. This disease is characterized by changes in retinal blood flow secondary to changes in the tone of retinal arterioles which is regulat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.013
更新日期：2013-10-01 00:00:00
abstract：:The relative distribution of acetylcholine (ACh) receptors on the surface of the isolated ocular lens of the rabbit was determined from induced changes in translens short-circuit current (I(SC)) and the translenticular resistance (R(t)) at seven delineated, parallel zones from the anterior to the posterior pole. For t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.1185
更新日期：2002-06-01 00:00:00
abstract：:Two previous studies have shown that N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of neuronal nitric oxide synthase, protects retinas of albino rats and mice from damaging levels of light. The aims of the present study were two-fold: (1) to confirm the protective effect of L-NAME on wild type albino rats ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00334-2
更新日期：2003-04-01 00:00:00
abstract：:When 15-day-old developing chick embryos were administered hydrocortisone hemisuccinate sodium (HC:0.25 mumol per egg) the concentration of glucose in both the aqueous humor and the vitreous body began to increase significantly after 3 hr and reached 24.4 +/- 0.3 mM and 22.3 +/- 0.9 mM, respectively, at 48 hr. Thereaf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80050-8
更新日期：1989-10-01 00:00:00
