Abstract:
:Anti-Vascular Endothelial Growth Factors (Anti-VEGF) agents have received recent interest as potential anti-fibrotic agents for their concurrent use with trabeculectomy. Preliminary cohort studies have revealed improved bleb morphology following trabeculectomy augmented with ranibizumab. The effects of this humanized monoclonal antibody on human Tenon's fibroblast (HTF), the key player of post trabeculectomy scar formation, are not fully understood. This study was conducted to understand the effects of ranibizumab on extracellular matrix production by HTF. The effect of ranibizumab on HTF proliferation and cell viability was determined using MTT assay (3-(4,5-dimethylthiazone-2-yl)-2,5-diphenyl tetrazolium). Ranibizumab at concentrations ranging from 0.01 to 0.5 mg/mL were administered for 24, 48 and 72 h in serum and serum free conditions. Supernatants and cell lysates from samples were assessed for collagen type 1 alpha 1 and fibronectin mRNA and protein level using quantitative real time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). After 48-h, ranibizumab at 0.5 mg/mL, significantly induced cell death under serum-free culture conditions (p < 0.05). Ranibizumab caused significant reduction of collagen type 1 alpha 1 (COL1A1) mRNA, but not for fibronectin (FN). Meanwhile, COL1A1 and FN protein levels were found upregulated in treated monolayers compared to control monolayers. Ranibizumab at 0.5 mg/mL significantly reduced cell viability in cultured HTF. From this study, we found that single application of ranibizumab is inadequate to induce the anti-fibrotic effects on HTF, suggesting the importance of adjunctive therapy. Further studies are underway to understand mechanism of actions of ranibizumab on HTF.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Md Noh SM,Sheikh Abdul Kadir SH,Bannur ZM,Froemming GA,Abdul Hamid Hasani N,Mohd Nawawi H,Crowston JG,Vasudevan Sdoi
10.1016/j.exer.2014.08.005subject
Has Abstractpub_date
2014-10-01 00:00:00pages
236-42eissn
0014-4835issn
1096-0007pii
S0014-4835(14)00226-7journal_volume
127pub_type
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(90)90169-u
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2003.08.006
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2010.02.010
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(90)90229-n
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.07.005
更新日期:2017-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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doi:10.1016/j.exer.2010.08.005
更新日期:2011-08-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2009.02.006
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doi:10.1016/j.exer.2018.08.013
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journal_title:Experimental eye research
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doi:10.1016/s0014-4835(05)80134-4
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2018.09.013
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0167
更新日期:1996-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0518
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pub_type: 杂志文章
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journal_title:Experimental eye research
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更新日期:2019-08-01 00:00:00
abstract::The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title:Experimental eye research
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