Abstract：
:Interleukin (IL)-1alpha and beta are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, we examined the effect of topical soluble IL-1 receptor antagonist on bone marrow-derived cell influx following corneal epithelial scrape injury in a mouse model. C57BL/6 mice underwent corneal epithelial scrape followed by application of IL-1 receptor antagonist (Amgen, Thousand Oaks, CA) at a concentration of 20 mg/ml or vehicle for 24 h prior to immunocytochemical detection of marker CD11b-positive cells into the stroma. In two experiments, topical IL-1 receptor antagonist had a marked effect in blocking cell influx. For example, in experiment 1, topical IL-1 receptor antagonist markedly reduced detectible CD11b-positive cells into the corneal stroma at 24h after epithelial injury compared with the vehicle control (3.5+/-0.5 (standard error of the mean) cells/400x field and 13.9+/-1.2 cells/400x field, respectively, p<0.01). A second experiment with a different observer performing cell counting had the same result. Thus, the data demonstrate conclusively that topical IL-1 receptor antagonist markedly down-regulates CD-11b-positive monocytic cell appearance in the corneal stroma. Topical IL-1 receptor antagonist could be an effective adjuvant for clinical treatment of corneal conditions in which unwanted inflammation has a role in the pathophysiology of the disorder.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Stapleton WM,Chaurasia SS,Medeiros FW,Mohan RR,Sinha S,Wilson SEdoi
10.1016/j.exer.2008.02.001subject
Has Abstractpub_date
2008-05-01 00:00:00pages
753-7issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(08)00045-6journal_volume
86pub_type
杂志文章abstract：:We showed previously that treatment of cultured rabbit lens epithelial cells (LECs) with hyperbaric oxygen (HBO) produced DNA strand-breaks, caused reversible inhibition of protein synthesis and induced the synthesis of a 32 kD protein. In the present work, we employed immunostaining procedures to identify the 32 kD p...
journal_title：Experimental eye research
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doi：10.1006/exer.1997.0356
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abstract：:The purpose of the current study was to localize the lens membrane protein, MIP 26, in nuclear fiber cells from different regions of aged normal and age-related cataractous human lenses. Adult, juvenile, fetal and embryonic nuclear regions in aged normal and age-related nuclear cataractous lenses were morphologically ...
journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2006.06.004
更新日期：2006-11-01 00:00:00
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journal_title：Experimental eye research
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更新日期：1992-01-01 00:00:00
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更新日期：2018-07-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/0014-4835(92)90058-z
更新日期：1992-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4835(90)90229-n
更新日期：1990-02-01 00:00:00
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更新日期：2006-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.021
更新日期：2006-04-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1993.1109
更新日期：1993-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：1990-11-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2001-01-01 00:00:00
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更新日期：2019-12-01 00:00:00
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更新日期：1983-02-01 00:00:00
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更新日期：1989-03-01 00:00:00
abstract：:Eosinophil infiltration is one of the aspects of the inflammatory response to an allergen. The aim of this study was to establish T-helper type-2 (Th2)-induced conjunctival eosinophilia in mice to evaluate the effect of anti-allergic drugs. Th2 clone, D10.G4.1. (D10) with its specific antigen, conalbumin was co-inject...
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journal_title：Experimental eye research
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更新日期：2006-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0829
更新日期：2000-05-01 00:00:00
