Hypertonic stress induces alpha B-crystallin expression.

abstract：:VEGF secretion by the human retinal pigment epithelium (hRPE) plays an important role in retinal and choroidal neovascularization. In this study, transforming growth factor-beta2 (TGF-beta2)-induced vascular endothelial growth factor (VEGF) gene expression was investigated in hRPE cells. Treatment of hRPE cells with T...

journal_title：Experimental eye research

authors： Bian ZM,Elner SG,Elner VM

更新日期：2007-05-01 00:00:00

abstract：:Prostaglandin E2 (PGE2) markedly reduces intraocular pressure (IOP) when applied topically and induces strong relaxation of pre-contracted isolated ciliary muscle through PGE2 receptor. Because the ciliary muscle relaxation reduces IOP by enhancing uveoscleral aqueous outflow, the ciliary muscle where the existence of...

journal_title：Experimental eye research

authors： Takamatsu M,Hotehama Y,Goh Y,Mishima HK

更新日期：2000-05-01 00:00:00

abstract：:Aceclidine can dissociate accommodative and outflow facility responses in monkeys and humans. We sought to determine if different muscarinic receptor subtypes control outflow facility, accommodative and pupillary responses to aceclidine in the living rhesus monkey eye, as a possible basis for this separation. Each eye...

journal_title：Experimental eye research

authors： Gabelt BT,Kaufman PL

更新日期：1994-05-01 00:00:00

abstract：:The purpose of present study is to dissect the role of PI3K/AKT signaling in the anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on rat retinal ganglion cells (RGCs) after optic nerve (ON) crush. The ONs of seventy-two adult male Wistar rats were crushed by a standardized method. Control ...

journal_title：Experimental eye research

authors： Tsai RK,Chang CH,Sheu MM,Huang ZL

更新日期：2010-05-01 00:00:00

abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...

journal_title：Experimental eye research

authors： Liu YF,Huang S,Ng TK,Liang JJ,Xu Y,Chen SL,Xu C,Zhang M,Pang CP,Cen LP

更新日期：2020-04-01 00:00:00

abstract：:Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotec...

journal_title：Experimental eye research

authors： Wang X,Mo X,Li D,Wang Y,Fang Y,Rong X,Miao H,Shou T

更新日期：2011-11-01 00:00:00

abstract：:Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In th...

journal_title：Experimental eye research

authors： Wei X,Zhang T,Yao Y,Zeng S,Li M,Xiang H,Zhao C,Cao G,Li M,Wan R,Yang P,Yang J

更新日期：2018-03-01 00:00:00

abstract：:The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...

journal_title：Experimental eye research

authors： McKown RL,Wang N,Raab RW,Karnati R,Zhang Y,Williams PB,Laurie GW

更新日期：2009-05-01 00:00:00

abstract：:The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of the pathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters. We studied the hen to determine ...

journal_title：Experimental eye research

authors： Martínez-García MC,Merayo-Llovés J,Blanco-Mezquita T,Mar-Sardaña S

更新日期：2006-10-01 00:00:00

abstract：:Tissue from the outflow system of six surgically enucleated aged eyes was used for an ultrastructural immunocytochemical study of the distribution of laminin and type IV collagen. The immunogold technique provided precise localization of laminin beneath lining endothelial cells of the inner wall of Schlemm's canal. La...

journal_title：Experimental eye research

authors： Marshall GE,Konstas AG,Lee WR

更新日期：1990-12-01 00:00:00

abstract：:The zebrafish retina regenerates in response to acute retinal lesions, replacing damaged neurons with new neurons. In this study we test the hypothesis that chronic stress to inner retinal neurons also triggers a retinal regeneration response in the bugeye zebrafish. Mutations in the lrp2 gene in zebrafish are associa...

journal_title：Experimental eye research

authors： Sherpa T,Hunter SS,Frey RA,Robison BD,Stenkamp DL

更新日期：2011-10-01 00:00:00

abstract：:Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) are all implicated in the development of neovascularization. To investigate the possible role of these factors in corneal neovascularization we have analysed the expression of MMP-2, MMP-9 and VEGF in a...

journal_title：Experimental eye research

authors： Kvanta A,Sarman S,Fagerholm P,Seregard S,Steen B

更新日期：2000-04-01 00:00:00

abstract：:The authors sought to evaluate the effect of linoleic acid hydroperoxide (18:2/LHP) in promoting choroidal neovascularization (CNV). Albino male rabbits received a subretinal injection of various amounts of LHP (ranging from 5 to 200 microg) dissolved in 50 microl of sodium borate buffer. Control eyes received the buf...

journal_title：Experimental eye research

authors： Tamai K,Spaide RF,Ellis EA,Iwabuchi S,Ogura Y,Armstrong D

更新日期：2002-02-01 00:00:00

abstract：:Identification of causal genetic defects for human diseases took a significant leap when the first generation DNA sequencing technologies enabled biologists extract sequence-based genetic information from living beings. However, these sequencing methods had unavoidable constraints of throughput, scalability, rapidity,...

journal_title：Experimental eye research

authors： Gupta S,Chatterjee S,Mukherjee A,Mutsuddi M

更新日期：2017-11-01 00:00:00

abstract：:We have previously shown that corticotropin-releasing hormone plays an important proinflammatory role in the induction of experimental autoimmune uveoretinitis. In this study, we examined the role of apoptosis in the destruction of the retina during experimental autoimmune uveoretinitis, and the role of corticotropin-...

journal_title：Experimental eye research

authors： Poulaki V,Mitsiades N,Mastorakos G,Caspi RR,Chrousos GP,Bouzas E

更新日期：2001-06-01 00:00:00

abstract：:Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...

journal_title：Experimental eye research

authors： Wederell ED,Brown H,O'connor M,Chamberlain CG,McAvoy JW,de Iongh RU

更新日期：2005-09-01 00:00:00

abstract：:The part of the light spectrum which is responsible for an increase of lenticular green autofluorescence upon white light exposure was determined for the rabbit. The increase of autofluorescence as a function of the light energy was measured, to assess a possible threshold energy for lens alterations. Thirteen rabbits...

journal_title：Experimental eye research

authors： Van Vreeswijk H,Boets EP,Van Best JA

更新日期：1993-03-01 00:00:00

abstract：:Experimental autoimmune uveoretinitis (EAU) in mice provides a useful platform to study the pathogenesis and experimental therapeutics of human uveitis. One often used EAU model employs C57BL/6 (B6) mice sensitized with a peptide residue having 1 to 20 amino acids of human interphotoreceptor retinoid binding protein (...

journal_title：Experimental eye research

authors： Tajiri N,Kato T,Satoh M,Iizuka M,Taniguchi M,Kitaichi N,Iwabuchi K

更新日期：2020-12-19 00:00:00

abstract：:The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...

journal_title：Experimental eye research

authors： Usui T,Kubo Y,Akanuma S,Hosoya K

更新日期：2013-08-01 00:00:00

abstract：:Anandamide (arachidonylethanolamide) is an endogenous ligand for cannabinoid receptors, and exerts various cannabimimetic activities. Since cannabinoids and anandamide were pharmacologically active with the eye, we examined metabolism of anandamide in a variety of porcine ocular tissues. In the presence of ethanolamin...

journal_title：Experimental eye research

authors： Matsuda S,Kanemitsu N,Nakamura A,Mimura Y,Ueda N,Kurahashi Y,Yamamoto S

更新日期：1997-05-01 00:00:00

abstract：:microPx-11, a ferriheme undecapeptide proteolytic degradation product of cytochrome C is shown to be a peroxidase with broad specificity degrading H2O2 and tertiary butyl hydroperoxide. It is also capable of effectively eliminating superoxide and hydroxyl radical. The peroxidase loses activity in the presence of perox...

journal_title：Experimental eye research

authors： Spector A,Ma W,Wang RR,Kleiman NJ

更新日期：1997-10-01 00:00:00

abstract：:Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....

journal_title：Experimental eye research

authors： Whiteley HE,Scott JR

更新日期：1990-10-01 00:00:00

abstract：:The extracellular matrix (ECM) plays a significant role in cellular and retinal health. The study of retinal tyrosine-sulfated proteins is an important first step toward understanding the role of ECM in retinal health and diseases. These secreted proteins are members of the retinal ECM. Tyrosine sulfation was shown to...

journal_title：Experimental eye research

authors： Kanan Y,Al-Ubaidi MR

更新日期：2015-04-01 00:00:00

abstract：:Age-related Macular Degeneration (AMD) is the leading cause of visual impairment and blindness in the elderly in developed countries. Neovascular/exudative (wet) AMD is the aggressive form of AMD and can involve choroidal neovascularization and vascular leakage. Anti-vascular endothelial growth factor (anti-VEGF) medi...

journal_title：Experimental eye research

authors： Giddabasappa A,Lalwani K,Norberg R,Gukasyan HJ,Paterson D,Schachar RA,Rittenhouse K,Klamerus K,Mosyak L,Eswaraka J

更新日期：2016-04-01 00:00:00

abstract：:Elevated permeability of retinal endothelial cells (REC), as observed in diabetic retinopathy (DR), is induced by extended exposure to ≥25 ng/ml vascular endothelial growth factor A165 (VEGF165) for up to 3 d and this effect is more pronounced when equimolar amounts of basic fibroblast growth factor (bFGF) and insulin...

journal_title：Experimental eye research

authors： Deissler HL,Deissler H,Lang GK,Lang GE

更新日期：2013-10-01 00:00:00

abstract：:Calf lens alpha-crystallin was isolated and the lysine residues were extensively modified with a variety of chemical agents. The effect of these modifications on elastase inhibitor activity, apparent molecular size, antibody reactivity and solubility were determined. The addition of either a methyl group or a threose ...

journal_title：Experimental eye research

authors： Ortwerth BJ,Olesen PR,Sharma KK,Prabhakaram M

更新日期：1993-01-01 00:00:00

abstract：:Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water solubl...

journal_title：Experimental eye research

authors： Nagaraj RH,Monnier VM

更新日期：1990-10-01 00:00:00

abstract：:Anti-VEGF-directed therapies have been a milestone for treating retinal vascular diseases. Depletion of monocyte lineage cells suppresses pathological neovascularization in the oxygen-induced retinopathy mouse model. However, the question whether myeloid-derived VEGF-A expression is responsible for the pathogenesis in...

journal_title：Experimental eye research

authors： Nürnberg C,Kociok N,Brockmann C,Lischke T,Crespo-Garcia S,Reichhart N,Wolf S,Baumgrass R,Eming SA,Beer-Hammer S,Joussen AM

更新日期：2018-01-01 00:00:00

abstract：:Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...

journal_title：Experimental eye research

authors： Gentleman S,Hemmings BA,Russell P,Chader GJ

更新日期：1989-06-01 00:00:00

abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...

journal_title：Experimental eye research

authors： Wikberg-Matsson A,Uhlén S,Wikberg JE

更新日期：2000-01-01 00:00:00