Abstract：
:Anti-VEGF-directed therapies have been a milestone for treating retinal vascular diseases. Depletion of monocyte lineage cells suppresses pathological neovascularization in the oxygen-induced retinopathy mouse model. However, the question whether myeloid-derived VEGF-A expression is responsible for the pathogenesis in oxygen-induced retinopathy remained unknown. We analyzed LysMCre-driven myeloid cell-specific VEGF-A knockout mice as well as mice with complete depletion of circulating macrophages through clodronate-liposome treatment in the oxygen-induced retinopathy model by immunohistochemistry, qPCR, and flow cytometry. Furthermore, we analyzed VEGF-A mRNA expression in MIO-M1 cells alone and in co-culture with BV-2 cells in vitro. The myeloid cell-specific VEGF-A knockout did not change relative retinal VEGF-A mRNA levels, the relative avascular area or macrophage/granulocyte numbers in oxygen-induced retinopathy and under normoxic conditions. We observed an insignificantly attenuated pathology in systemically clodronate-liposome treated knockouts but evident VEGF-A expression in activated Müller cells on immunohistochemically stained sections. MIO-M1 cells had significantly higher expression levels of VEGF-A in co-culture with BV-2 cells compared to cultivating MIO-M1 cells alone. Our data show that myeloid-derived cells contribute to pathological neovascularization in oxygen-induced retinopathy through activation of VEGF-A expression in Müller cells.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Nürnberg C,Kociok N,Brockmann C,Lischke T,Crespo-Garcia S,Reichhart N,Wolf S,Baumgrass R,Eming SA,Beer-Hammer S,Joussen AMdoi
10.1016/j.exer.2017.10.011subject
Has Abstractpub_date
2018-01-01 00:00:00pages
56-69eissn
0014-4835issn
1096-0007pii
S0014-4835(17)30458-Xjournal_volume
166pub_type
杂志文章abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...
journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0116
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pub_type： 杂志文章
doi：10.1006/exer.2000.0865
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.011
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.013
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journal_title：Experimental eye research
pub_type： 杂志文章
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