Abstract：
:Docosahexaenoic acid is the major polyunsaturated fatty acid in photoreceptor membrane phospholipids and is thought to be necessary for photoreceptor function. Docosahexaenoic acid may be metabolized to docosanoids or, by retroconversion, to eicosapentaenoic acid followed by lipoxygenation and synthesis of n-3 metabolites. In this study we investigated the time- and illuminance-dependent release of docosahexaenoic acid from photoreceptor phospholipids in the rat retina in vitro and the effects of the phospholipase A2 inhibitor, quinacrine, on this release. Isolated rat retinae were incubated in oxygenated Ringer-Bicarbonate-Glucose-Medium and labelled with [3H]docosahexaenoic acid for 180 min in darkness. The incorporation of [3H]docosahexaenoic acid into retinal phospholipids was monitored by thin-layer chromatography. The release of [3H]docosahexaenoic acid was determined under illuminances of 100, 500, 1000, 5000 and 10,000 lx for 10 min, illuminance durations of 0.25, 2, 5 and 10 min at 10,000 lx, and with the addition of 10 and 100 mumol quinacrine to the incubation medium at 10,000 lx for 10 min. Our results demonstrate a release of docosahexaenoic acid from retinal phospholipids that is finely tuned by light levels and exposure duration. The kinetics of the time dependent docosahexaenoic acid release and the effects of quinacrine suggest that this release is mediated in part by activation of phospholipase A2. The light-elicited docosahexaenoic acid release may serve as a protective measure against formation of prostaglandins by inhibiting cyclooxygenase and by promoting the synthesis of less potent leukotrienes of the 5-series via retroconversion to eicosapentaenoic acid and 5-lipoxygenation.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Reinboth JJ,Clausen M,Remé CEdoi
10.1006/exer.1996.0116subject
Has Abstractpub_date
1996-09-01 00:00:00pages
277-84issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90116-5journal_volume
63pub_type
杂志文章abstract：:Paired human lenses ranging from 2 to 96 yr old (n = 17) were harvested within 2 hr post-mortem and immersed in liquid nitrogen. Phosphorus containing metabolites from perchloric acid extracts were quantitated using phosphorus-31 nuclear magnetic resonance spectroscopy. Low-energy metabolites detected include the phos...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90016-8
更新日期：1991-06-01 00:00:00
abstract：:We aim to determine whether lymphatic drainage from the eye changes with age. Using quantitative photoacoustic tomography, groups of young and older mice were studied in the live state. 10 CD-1 mice of 2-3 months (5M/5F) were studied in addition to 13 older mice of 12-13 months (6M/7F). In each of 23 mice, near-infrar...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108029
更新日期：2020-05-01 00:00:00
abstract：:Axonal cytoskeleton proteins are intrinsically related to retinal ganglion cell (RGC) health and disease. This study quantifies and documents the sectoral distribution of axonal cytoskeleton proteins in the different laminar compartments of the normal human optic nerve head (ONH). Nine human eyes were used (mean age o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.10.006
更新日期：2014-11-01 00:00:00
abstract：:Basic fibroblast growth factor (bFGF) has been shown to rescue dying photoreceptor cells in the RCS rat, a model with a genetic defect of the RPE that impairs outer segment phagocytosis. The purpose of the present study was to determine whether intravitreal injection of bFGF would have a similar effect on photorecepto...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0147
更新日期：1996-11-01 00:00:00
abstract：:Mechanical failure of the ciliary zonule characterizes several ocular and systemic diseases. The mouse has emerged as a useful model system to investigate the composition and structure/function relationships of the zonule. However, visualizing the organization of the diaphanous fibers that comprise the zonule is techn...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.05.025
更新日期：2019-08-01 00:00:00
abstract：:Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvast...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.05.013
更新日期：2013-08-01 00:00:00
abstract：:Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) are all implicated in the development of neovascularization. To investigate the possible role of these factors in corneal neovascularization we have analysed the expression of MMP-2, MMP-9 and VEGF in a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0790
更新日期：2000-04-01 00:00:00
abstract：:The presence of cartilage-matrix molecules in the embryonic chick eye was shown by means of measuring RNA expression and immunostaining studies. The molecules examined were proteoglycan core protein (PG-core), link protein (LP), cartilage-matrix protein (CMP) and type II collagen. The genes encoding these proteins are...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80061-7
更新日期：1988-05-01 00:00:00
abstract：:Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1109
更新日期：1993-08-01 00:00:00
abstract：:The ocular hypotensive effect of topically applied prostaglandins (PGs) is well documented. Although PGs introduced in the posterior chamber accumulate in the anterior tissues (e.g. iris/ciliary complex), little is known about the metabolism of PGs by these tissues. We have recently found that non-pigmented epithelial...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90202-4
更新日期：1992-08-01 00:00:00
abstract：:The causal mechanism of light damage in the albino rat retina has been investigated. Male and female albino Wistar rats weighing 200-300 g and previously maintained in normal room lighting of approximately 15 hr light (0.85 +/- 0.05 X 10(-4) W cm-2) per 9 hr dark cycle were simultaneously exposed continuously to unifo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90012-6
更新日期：1986-01-01 00:00:00
abstract：:The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.07.005
更新日期：2017-10-01 00:00:00
abstract：:Rho GTPase regulated contractile signaling in the trabecular meshwork (TM) has been shown to modulate aqueous humor (AH) outflow and intraocular pressure (IOP). To explore whether elevated IOP, a major risk factor for primary open angle glaucoma (POAG) influences Rho GTPase signaling in the TM, we recorded AH outflow ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.05.001
更新日期：2015-07-01 00:00:00
abstract：:The effect of acute exposure to various intensities of white light on visual behavior and retinal structure was evaluated in the T4R RHO dog, a naturally-occurring model of autosomal dominant retinitis pigmentosa due to a mutation in the Rhodopsin gene. A total of 14 dogs (ages: 4-5.5 months) were used in this study: ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.04.006
更新日期：2016-05-01 00:00:00
abstract：:The distribution of chondroitin sulfate proteoglycans in the mouse interphotoreceptor matrix (IPM) proximal to the retinal pigment epithelium (RPE) was evaluated with EM histochemical techniques using Cupromeronic Blue (CmB) before and after retinal separation. Densely packed, sheet-like processes surrounding vesicle-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90054-v
更新日期：1992-03-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
abstract：:The proteasomal pathway is responsible for processes essential for cell viability, including the selective degradation of oxidized proteins. An age-dependent loss in proteasome function has been reported in many tissues, but has not been examined in the retina. In this study, we evaluated proteasome function and prote...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:The purpose of this study was to investigate the expression of the EGF family of growth factors and EGF receptor subtypes (ErbB1-4) present in lacrimal gland and determine the effects of these growth factors on different functions of rat lacrimal gland. RT-PCR was used to detect mRNA expression in the lacrimal gland o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.10.006
更新日期：2005-03-01 00:00:00
abstract：:VEGF secretion by the human retinal pigment epithelium (hRPE) plays an important role in retinal and choroidal neovascularization. In this study, transforming growth factor-beta2 (TGF-beta2)-induced vascular endothelial growth factor (VEGF) gene expression was investigated in hRPE cells. Treatment of hRPE cells with T...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.12.016
更新日期：2007-05-01 00:00:00
abstract：:Owing to a rapidly aging population, vision impairment caused by age-related cataract has become very common. Age-related cataract has also become one of the principal causes of blindness, and apoptosis of lens epithelial cells contributes to non-congenital cataract development. Previous studies have reported that mic...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.04.018
更新日期：2016-06-01 00:00:00
abstract：:In order to study acute retinal toxicity of intravitreal triamcinolone acetonide (TA) at high doses in an animal model, thirty New Zealand albino rabbits were injected with intravitreal TA. The animals were divided in five groups: Group 1 received an intravitreal injection of 0.1 mL balanced salt solution; Group 2, 0....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.10.006
更新日期：2007-02-01 00:00:00
abstract：:The distribution of collagen degradation and synthesis (turnover) between the central and peripheral areas of clear corneal allografts was determined, following transplantation of 6-mm [3H]proline-labelled corneal buttons from extensively pre-labelled, young rabbits into 13 nonradioactive mature rabbits. Control, cont...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80076-3
更新日期：1987-07-01 00:00:00
abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1175
更新日期：1993-12-01 00:00:00
abstract：:Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprag...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.12.001
更新日期：2011-03-01 00:00:00
abstract：:Maintenance of cellular calcium levels is critical to cell function. Loss of calcium homeostasis might be a contributing factor to the development of cataract in the lens. In lens epithelium, calcium is involved in cell signaling and its precise regulation is vital. In this study, we investigated the regulation of sar...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.2049
更新日期：2002-11-01 00:00:00
abstract：:A number of variants of the wild-type (wt) estrogen receptor beta (ERbeta-1) coexist in a wide range of tissues. In the human these include, together with others, the expression of several isoforms (ERbeta-2-ERbeta-5) due to alternative splicing of exons encoding the carboxy terminus. In this study, we determined whet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.019
更新日期：2005-08-01 00:00:00
abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107955
更新日期：2020-04-01 00:00:00
abstract：:Eosinophil infiltration is one of the aspects of the inflammatory response to an allergen. The aim of this study was to establish T-helper type-2 (Th2)-induced conjunctival eosinophilia in mice to evaluate the effect of anti-allergic drugs. Th2 clone, D10.G4.1. (D10) with its specific antigen, conalbumin was co-inject...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0677
更新日期：1999-07-01 00:00:00
abstract：:Nitric oxide (NO) production by vascular endothelium is important in regulation of blood flow. Reduced production of NO can adversely affect blood flow and other vascular functions. We investigated the expression of three nitric oxide synthase (NOS) isoforms in retina and choroid of aged human eyes and eyes with AMD. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.10.004
更新日期：2010-01-01 00:00:00
abstract：:The transcription factor Prox1 acts in rodent retinogenesis, at least in promoting cell cycle withdrawal and horizontal cell production. In the mature retina, this protein is detected at the inner nuclear layer of all vertebrate groups. We have made a neurochemical characterisation of Prox1(+) cell types in two differ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.10.015
更新日期：2010-02-01 00:00:00
