Abstract：
:Maintenance of cellular calcium levels is critical to cell function. Loss of calcium homeostasis might be a contributing factor to the development of cataract in the lens. In lens epithelium, calcium is involved in cell signaling and its precise regulation is vital. In this study, we investigated the regulation of sarco/endoplasmic reticulum Ca(2+)-ATPase2b (SERCA2b) and SERCA3 isoform expression in cultured epithelial B-3 cells from human lenses. Both mRNA and membrane proteins samples were collected for semi quantitative RT-PCR using GAPDH as a control. Western blot analyses were performed on membrane samples.Thapsigargin, a SERCA isoform inhibitor which causes increased cytosolic levels elicited dose-and time-dependent up-regulation of SERCA3 at both mRNA and protein levels; SERCA2b expression was unaffected. Both EGTA and actinomycin partially inhibited the thapsigargin-induced SERCA3 up-regulation. These results indicate that the up-regulation of SERCA3 by thapsigargin is dependent on a calcium-mediated pathway that is likely to occur at the transcription level.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Liu L,Paterson CA,Borchman Ddoi
10.1006/exer.2002.2049keywords:
subject
Has Abstractpub_date
2002-11-01 00:00:00pages
583-90issue
5eissn
0014-4835issn
1096-0007pii
S001448350292049Xjournal_volume
75pub_type
杂志文章abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title：Experimental eye research
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abstract：:Certain mutations in optineurin (gene OPTN) are associated with primary open angle glaucoma. Optineurin is ubiquitously expressed but it shows high level of expression in certain cells and tissues including retinal ganglion cells. It interacts with many proteins, often acting as an adaptor to link two or more proteins...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
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journal_title：Experimental eye research
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pub_type： 更正并重新发布的文章,杂志文章
doi：
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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journal_title：Experimental eye research
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doi：10.1006/exer.1994.1099
更新日期：1994-08-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.020
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更新日期：2018-05-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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