Abstract：
:Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that the N-terminal residue is acetylated. Thus, gamma S-crystallin is similar to the acidic beta-crystallins in having a flexible N-terminal extension and an N-terminus that is blocked with an acetyl group but no C-terminal extension. In addition to the short N-terminal extension in gamma S-crystallin, other unassigned resonances are also observed in the NMR spectra. In gamma B-crystallin, however, cross-peaks in the NH to alpha-CH region of the spectrum are essentially restricted to the last three residues of the C-terminal domain. The NMR data imply that gamma S-crystallin has a more flexible structure than gamma B-crystallin. Sedimentation equilibrium studies on gamma S-crystallin are consistent with this proposal. Resonances from the N-terminal extension of gamma S-crystallin are not affected by the presence of alpha-crystallin implying that this region is not involved in interactions between the two molecules. It is concluded that gamma S-crystallin shares structural properties which are intermediate between the beta- and gamma-crystallins.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Cooper PG,Carver JA,Aquilina JA,Ralston GB,Truscott RJdoi
10.1006/exer.1994.1099subject
Has Abstractpub_date
1994-08-01 00:00:00pages
211-20issue
2eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71099-2journal_volume
59pub_type
杂志文章abstract：:Preeclampsia (PE) is a hypertensive complication of pregnancy. Its cause is still unknown and it could be a risk factor for future ophthalmic problems. Retinal vascular bed alterations have been described as a consequence of PE, suggesting a retinopathy. Factors related to angiogenesis and vascular permeability, such ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107981
更新日期：2020-04-01 00:00:00
abstract：:This study demonstrates post-translational arginylation of bovine serum albumin and endogenous lens proteins by bovine lens arginyl-tRNA:protein transferase. This reaction has been proposed to be the first step in marking specific proteins for degradation by the non-lysosomal, ATP-dependent, ubiquitin-mediated proteol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90220-9
更新日期：1991-11-01 00:00:00
abstract：:Anti-VEGF-directed therapies have been a milestone for treating retinal vascular diseases. Depletion of monocyte lineage cells suppresses pathological neovascularization in the oxygen-induced retinopathy mouse model. However, the question whether myeloid-derived VEGF-A expression is responsible for the pathogenesis in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.10.011
更新日期：2018-01-01 00:00:00
abstract：:A prior study found that inhibition of nitric oxide synthase with L-NAME causes a large, rapid decrease in IOP in anesthetized rabbits. In this follow-up study we sought to determine if this hypotensive effect was due to decreased aqueous production, possibly caused by ciliary vasoconstriction. Two protocols were perf...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1050
更新日期：2001-09-01 00:00:00
abstract：:miRNA-7a plays critical roles in various biological aspects in health and disease. We aimed to reveal roles of miR-7a in mouse retinal development by loss- and gain-of-function analyses of miR-7a. Plasmids encoding miR-7a or miR-7a-decoy (anti-sense miR-7a) were introduced into mouse retina at P0, and the retina was c...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.022
更新日期：2015-09-01 00:00:00
abstract：:Much controversy has arisen on the nature and sources of stem cells in the adult human retina. Whilst ciliary epithelium has been thought to constitute a source of neural stem cells, a population of Müller glia in the neural retina has also been shown to exhibit neurogenic characteristics. This study aimed to compare ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.015
更新日期：2011-12-01 00:00:00
abstract：:Changes in the distribution of keratan sulfate in the anterior segment of developing ocular tissue were investigated histochemically and immunohistochemically using human eyes from 5 weeks gestation to 2 years after birth. Enzyme digestion methods with streptomyces hyaluronidase, chondroitinase and keratanase showed t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1018
更新日期：1994-03-01 00:00:00
abstract：:Tear samples were collected from 46 healthy volunteers evenly distributed according to sex and age (mean age 43.5 years). Samples were denatured in a Tris-HCl sample buffer containing 2-mercaptoethanol and SDS, and applied to a gradient SDS-polyacrylamide gel for electrophoresis. The proteinaceous material was transfe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90160-g
更新日期：1991-10-01 00:00:00
abstract：:SLC4A11 mutations are associated with Fuchs' endothelial corneal dystrophy (FECD), congenital hereditary endothelial dystrophy (CHED) and Harboyan syndrome (endothelial dystrophy with auditory deficiency). Mice with genetically ablated Slc4a11 recapitulate CHED, exhibiting significant corneal edema and altered endothe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.12.003
更新日期：2019-03-01 00:00:00
abstract：:There is increasing evidence that the macular pigment carotenoids, lutein and zeaxanthin, may play an important role in the prevention of age-related macular degeneration, cataract, and other blinding disorders. Although it is well known that the retina and lens are enriched in these carotenoids, relatively little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0954
更新日期：2001-03-01 00:00:00
abstract：:Vision loss due to various forms of outer retinal degeneration remains a major problem in clinical ophthalmology. Most retinal degenerations are precipitated by genetic mutations affecting the retinal pigment epithelium and sensory retina, but it is becoming increasingly evident that resultant metabolic changes within...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2005.01.018
更新日期：2005-06-01 00:00:00
abstract：:The response of the poikilothermal lens to various incubation temperatures in vitro was compared with that of the homothermal lens. The rainbow trout lens was used as the poikilothermal lens and the rat lens as the homothermal lens. In contrast to rat lenses, cataract developed at 37 degrees C in rainbow trout lenses,...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90021-1
更新日期：1985-08-01 00:00:00
abstract：:Immunofluorescent imaging is an indispensable technique to study morphology and molecular aspects in tissues. Classical approaches make it necessary to cut physical sections of tissue samples to overcome the limited penetration depth of light, restricting the available information to two dimensions. Recent advances in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.12.001
更新日期：2019-03-01 00:00:00
abstract：:Both in vivo and in vitro models were employed in the present study to assess the relative contribution of osmotic stress and increasing calcium levels to the development of sugar cataracts. In galactose cataract obtained from galactosemic weanling rats, the concentration of total calcium increased by nearly 10% at th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0486
更新日期：1998-06-01 00:00:00
abstract：:Analysis of bovine interphotoreceptor matrix and conditioned medium from human Y-79 retinoblastoma cells by gelatin SDS-PAGE zymography reveals abundant activity of a 72-kDa M(r) gelatinase. The 72-kDa gelatinase from either source is inhibited by EDTA but not aprotinin or NEM, indicating that it is a metalloproteinas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1107
更新日期：1994-09-01 00:00:00
abstract：:Retinal degeneration (RD) results from photoreceptor apoptosis. Cell transplantation, one potential therapeutic approach, requires expandable stem cells that can form mature photoreceptors when differentiated. Freshly dissociated primary retinal cells from postnatal day 2-6 (PN2-6) mouse retina can give rise, post-tra...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:Certain mutations in optineurin (gene OPTN) are associated with primary open angle glaucoma. Optineurin is ubiquitously expressed but it shows high level of expression in certain cells and tissues including retinal ganglion cells. It interacts with many proteins, often acting as an adaptor to link two or more proteins...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.08.020
更新日期：2016-03-01 00:00:00
abstract：:Tears serve as a viable diagnostic fluid with advantages including less invasive sample to collect and less complex to prepare for analysis. Several water-soluble and fat-soluble vitamins were detected and quantified in human tears and compared with blood serum levels. Samples from 15 family pairs, each pair consistin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.12.007
更新日期：2017-02-01 00:00:00
abstract：:Electrical field stimulation produced contractions of the rabbit isolated iris sphincter muscle which were completely blocked by tetrodotoxin (10(-6) M). Low frequency (0.16-5 Hz) responses were atropine-resistant, but were prevented by sensory (trigeminal) denervation, and were considered to be owing to antidromic st...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90099-x
更新日期：1984-02-01 00:00:00
abstract：:RPE65-related Leber's congenital amaurosis (LCA) is a rod-cone dystrophy whose clinical outcome is mainly attributed to the loss of rod photoreceptors followed by cone degeneration. Pathogenesis in Rpe65(-/-) mice is characterized by a slow and progressive degeneration of rods dependent on the constitutive activation ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.12.019
更新日期：2012-03-01 00:00:00
abstract：:The purpose of this work was to investigate the effect of c-fos antisense oligonucleotide (c-fos-AS-ON) on proliferative vitreoretinopathy (PVR). Cultures of human retinal pigment epithelial (hRPE) cells were established from adult human corneal donors. These cells were positively stained for cytokeratins. C-fos-AS-ON...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.07.020
更新日期：2006-12-01 00:00:00
abstract：:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increase...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
abstract：:The goal of this study was to investigate the efflux of K(+) from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K(+) channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm(2) UVB, fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.02.019
更新日期：2011-05-01 00:00:00
abstract：:The Otx2 gene encodes a transcription factor essential for the normal development of brain, cerebellum, pineal gland, and eye. In the retina, Otx2 has essential functions from early embryogenesis to adulthood. As soon as the optic vesicle is formed, the gene is required for retinal pigment epithelium specification. Ot...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.03.007
更新日期：2013-06-01 00:00:00
abstract：:This study presents a computational model of the growth of the normal human lens and the induction of spoke-like cortical cataract in the aging lens. The anterior lens is modelled as a 2-D disk with a circumferential germinative zone. Lens cortical fibre cells in the same generation cover the surface in three identica...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0883
更新日期：2000-09-01 00:00:00
abstract：:The transcription factor Brn3a has been reported to be a good marker for adult rat retinal ganglion cells in control and injured retinas. However, it is still unclear if Brn3a expression declines progressively by the injury itself or otherwise its expression is maintained in retinal ganglion cells that, though being i...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.02.001
更新日期：2011-04-01 00:00:00
abstract：:The Raman microspectroscopic method was used to determine the local water and protein content in human lenses. In 18 lenses of varying age position-defined water/protein content measurements were carried out along the visual and the equatorial axis. A main characteristic of the human lens is its constant and relativel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90079-t
更新日期：1991-08-01 00:00:00
abstract：:Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stain...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90111-8
更新日期：1984-07-01 00:00:00
abstract：:N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.018
更新日期：2019-04-01 00:00:00
abstract：:The scleral spur in 37 human (age 17-87 years) and six cynomolgus monkey eyes (2-4 years) was investigated. Serial meridional and tangential sections were studied with ultrastructural and immunocytochemical methods. The bundles of the ciliary muscle do not enter the scleral spur, but their tendons, which consist of el...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90132-c
更新日期：1992-04-01 00:00:00
