Abstract:
:Analysis of bovine interphotoreceptor matrix and conditioned medium from human Y-79 retinoblastoma cells by gelatin SDS-PAGE zymography reveals abundant activity of a 72-kDa M(r) gelatinase. The 72-kDa gelatinase from either source is inhibited by EDTA but not aprotinin or NEM, indicating that it is a metalloproteinase (MMP). The 72-kDa MMP is converted to a 62-kDa species with APMA treatment after gelatin sepharose affinity purification, typical of previously described gelatinase MMP-2. The latent 72-kDa gelatinase from either bovine IPM or Y-79 media autoactivates without APMA in the presence of calcium and zinc after 72 hr at 37 degrees C, producing a fully active mixture of proteinase species, 50 (48 in Y-79 medium), 38 and 35 kDa in size. The presence of inhibitory activity was examined in both whole bovine IPM and IPM fractions separated by SDS-PAGE. Whole IPM inhibited gelatinolytic activity of autoactivated Y-79-derived MMP in a dose-dependent manner. Inhibitory activities are observed in two protein fractions of 27-42 and 20-25 kDa. Western blots using antibodies to human tissue inhibitor of metalloproteinase 1 and 2 (TIMP-1 and -2) reveal the presence of two TIMP-1-like proteins at 21 and 29 kDa in inhibitory fractions of the bovine IPM. TIMP-2 was not detected in the inhibitory IPM fractions, consistent with the observed autoactivation of bovine IPM 72-kDa gelatinase. Potential roles for this IPM MMP-TIMP system include physiologic remodelling of the neural retina-RPE cell interface and digestion of shed rod outer segment as well as pathological processes such as retinal detachment, PE cell migration, neovascularization and tumor progression. Cultured Y-79 cells appear to be a good model for studying the production and regulation of this proteinase system.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Jones BE,Moshyedi P,Gallo S,Tombran-Tink J,Arand G,Reid DA,Thompson EW,Chader GJ,Waldbillig RJdoi
10.1006/exer.1994.1107subject
Has Abstractpub_date
1994-09-01 00:00:00pages
257-69issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71107-9journal_volume
59pub_type
杂志文章abstract::The causes of vitreous humor (VH) liquefaction remain unclear. Diabetes accelerates this process and other ocular diseases. The weakening of the blood-retina barrier observed with diabetes could enhance the rate of transfer of relatively small molecules such as glucose (Glu) and phospholipids (PLs) from the retina to ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108221
更新日期:2020-11-01 00:00:00
abstract::The purpose of this study was to determine whether endothelin B (ETB) receptor levels in the optic nerve are related to retinal ganglion cell (RGC) loss in a model of chronic endothelin-1 (ET-1) induced optic neuropathy. RGCs of adult Brown Norway rats were first retrogradely labeled with fluorochrome from the superio...
journal_title:Experimental eye research
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更新日期:2009-03-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/s0014-4835(88)80083-6
更新日期:1988-02-01 00:00:00
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abstract::It has been reported that chymase activity was increased in allergic conjunctivitis patients and this activity was correlated with the severity of the disease. However, the precise roles of chymase in allergic conjunctivitis are unclear, and whether chymase inhibitors are effective for allergic conjunctivitis has not ...
journal_title:Experimental eye research
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更新日期:2013-08-01 00:00:00
abstract::In response to injury, the adult mammalian retina shows signs of structural remodeling, possibly in an attempt to preserve or regain some of its functional neural connections. In order to study the mechanisms involved in injury-induced plasticity, we have studied changes in growth associated protein 43 (GAP-43) after ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.006
更新日期:2007-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80059-4
更新日期:1989-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0250
更新日期:1997-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80008-8
更新日期:1987-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.08.021
更新日期:2008-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2004-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.08.011
更新日期:2017-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.01.004
更新日期:2010-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90025-4
更新日期:1986-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1061
更新日期:1993-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.05.020
更新日期:2015-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.09.005
更新日期:2012-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2018.08.001
更新日期:2018-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.02.019
更新日期:2019-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.06.022
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2003.12.010
更新日期:2004-05-01 00:00:00
abstract::Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90048-1
更新日期:1989-09-01 00:00:00
abstract::Familial exudative vitreoretinopathy (FEVR) is a disease exhibits a wide range of clinical signs, ranging mild peripheral retinal vascular anomalies to severe retinal detachments. Individuals with mild FEVR are frequently asymptomatic with good visual function and are often undiagnosed. However, little is known about ...
journal_title:Experimental eye research
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更新日期:2020-03-01 00:00:00
abstract::Choroidal neovascularization (CNV) is one of the severe pathological consequences of the end-stage of age-related macular degeneration (AMD). Several lines of evidence implicate increased levels of vascular endothelial growth factor (VEGF) in the retinas of AMD patients. Current available agents for the inhibition of ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.06.019
更新日期:2010-09-01 00:00:00
abstract::Vitreous from bovine, human and chick embryo has been found to contain a trypsin inhibitory activity. Chymotrypsin-inhibitory activity was also identified in bovine and chick embryo vitreous. Following either ultrafiltration or Bio Gel P-10 chromatography, these activities appear in fractions having a molecular weight...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90031-4
更新日期:1985-11-01 00:00:00
abstract::Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.06.022
更新日期:2010-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90116-7
更新日期:1984-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1006
更新日期:1994-02-01 00:00:00
abstract::Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90183-0
更新日期:1984-06-01 00:00:00
abstract::Numerous animal studies indicate that aldose reductase inhibitors (ARIs) are beneficial for the prevention or amelioration of diabetic complications such as neuropathy, nephropathy and the ocular complications of cataract, retinopathy and keratopathy. To aid in the identification of novel potent ARIs, we have previous...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.011
更新日期:2004-12-01 00:00:00