Abstract:
:Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. aureus alpha-hemolysin (alpha-toxin) in corneal epithelial wound healing and infectious keratitis in the setting of a corneal wound. The effect of wild-type and isogenic Hla mutant (α-hemolysin gene deleted) S. aureus bacteria and conditioned media on corneal epithelial wound healing was tested in vitro using a scratch assay and in vivo using a murine epithelial debridement model. The invasiveness of wild-type and Hla mutant S. aureus was evaluated in vitro in human corneal epithelial cells and in vivo in a murine model of infectious keratitis following total epithelial debridement. S. aureus and its conditioned media significantly delayed epithelial wound closure both in vitro (P < 0.05) and in vivo (P < 0.05). The effect of S. aureus on wound healing was significantly diminished with the Hla mutant strain (P < 0.05). Likewise, compared to the wild-type strain, the Hla mutant strain demonstrated significantly reduced ability to invade corneal epithelial cells in vitro (P < 0.05) and infect murine corneas following total epithelial debridement in vivo (P < 0.05). In conclusion, S. aureus alpha-hemolysin plays a major role in the pathologic modulation of corneal epithelial wound healing and the intracellular invasion of the bacteria. Limiting colonization by S. aureus and/or blocking alpha-hemolysin may provide a therapeutic approach for corneal wound healing and infectious disorders.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Putra I,Rabiee B,Anwar KN,Gidfar S,Shen X,Babalooee M,Ghassemi M,Afsharkhamseh N,Bakhsh S,Missiakas D,Nezamabadi A,Milani B,Eslani M,Djalilian ARdoi
10.1016/j.exer.2019.02.019subject
Has Abstractpub_date
2019-04-01 00:00:00pages
263-270eissn
0014-4835issn
1096-0007pii
S0014-4835(18)30592-Xjournal_volume
181pub_type
杂志文章abstract::Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1144
更新日期:1994-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1117
更新日期:1994-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.05.010
更新日期:2019-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2010.02.010
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journal_title:Experimental eye research
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abstract:PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:1990-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00