Differential effects of the EGF family of growth factors on protein secretion, MAPK activation, and intracellular calcium concentration in rat lacrimal gland.

abstract：:A serum-free culture of chicken retinal pigmented epithelial cells has been established in order to analyse how cell-substrate interactions or environmental factors affect the process of transdifferentiation into lens cells from pigmented epithelial cells. The serum-free culture medium for chicken pigmented epithelial...

journal_title：Experimental eye research

authors： Kosaka J,Watanabe K,Eguchi G

更新日期：1992-08-01 00:00:00

abstract：:We have investigated the biochemical and cell biological basis of the reported beneficiary effects of the leaf extracts of the plant Ginkgo biloba, which has been used as a possible ophthalmic drug. The antioxidant, antimicrobial, anti-apoptotic and cytoprotective properties of the standardized extract called EGb761 w...

journal_title：Experimental eye research

authors： Thiagarajan G,Chandani S,Harinarayana Rao S,Samuni AM,Chandrasekaran K,Balasubramanian D

更新日期：2002-10-01 00:00:00

abstract：:Rat lenses incubated in tissue culture medium (M 199) maintain their transparency for a long period of time. The soluble corticosteroid, solumedrol (methyl prednisolone sodium succinate) was added to the medium, at concentrations including the range expected during rejection episodes following organ transplantation (3...

journal_title：Experimental eye research

authors： Creighton MO,Sanwal M,Stewart-DeHaan PJ,Trevithick JR

更新日期：1983-07-01 00:00:00

abstract：:Calf lens alpha-crystallin was isolated and the lysine residues were extensively modified with a variety of chemical agents. The effect of these modifications on elastase inhibitor activity, apparent molecular size, antibody reactivity and solubility were determined. The addition of either a methyl group or a threose ...

journal_title：Experimental eye research

authors： Ortwerth BJ,Olesen PR,Sharma KK,Prabhakaram M

更新日期：1993-01-01 00:00:00

abstract：:Preretinal pH was measured in the avascular region of 19 rabbit retinas during alternating cycles of light and dark, using a miniature, needle-type pH electrode. In 14 experiments with the rabbits breathing room air, cyclic changes in pH, decreasing in the dark and increasing in the light, were observed. The transitio...

journal_title：Experimental eye research

authors： Murray DL,Feke GT,Weiter JJ

更新日期：1991-12-01 00:00:00

abstract：:The ocular hypotensive effect of topically applied prostaglandins (PGs) is well documented. Although PGs introduced in the posterior chamber accumulate in the anterior tissues (e.g. iris/ciliary complex), little is known about the metabolism of PGs by these tissues. We have recently found that non-pigmented epithelial...

journal_title：Experimental eye research

authors： Asakura T,Shichi H

更新日期：1992-08-01 00:00:00

abstract：:We previously demonstrated that there are two waves of increased collagen synthesis following corneal laceration in rabbits. In the present study, we have examined whether increases in collagen synthesis and degradation result from increased amounts of mRNAs for collagen and collagenase, respectively. Rabbits were ane...

journal_title：Experimental eye research

authors： Sakai J,Hung J,Zhu G,Katakami C,Boyce S,Kao WW

更新日期：1991-03-01 00:00:00

abstract：:Experimental autoimmune uveoretinitis (EAU) in mice provides a useful platform to study the pathogenesis and experimental therapeutics of human uveitis. One often used EAU model employs C57BL/6 (B6) mice sensitized with a peptide residue having 1 to 20 amino acids of human interphotoreceptor retinoid binding protein (...

journal_title：Experimental eye research

authors： Tajiri N,Kato T,Satoh M,Iizuka M,Taniguchi M,Kitaichi N,Iwabuchi K

更新日期：2020-12-19 00:00:00

abstract：:A method for the isolation and purification of human ocular mucin from the brief saline extract of human ocular mucus is reported. Initial purification of ocular mucin was achieved by sequential chromatography of the saline-soluble mucus extract from an individual donor's mucus pool on columns of Sephadex G-50 and Sep...

journal_title：Experimental eye research

authors： Chao CC,Butala SM,Herp A

更新日期：1988-08-01 00:00:00

abstract：:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...

journal_title：Experimental eye research

authors： Hicks D,Molday RS

更新日期：1986-01-01 00:00:00

abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...

journal_title：Experimental eye research

authors： Liu YF,Huang S,Ng TK,Liang JJ,Xu Y,Chen SL,Xu C,Zhang M,Pang CP,Cen LP

更新日期：2020-04-01 00:00:00

abstract：:The transcription factor Prox1 acts in rodent retinogenesis, at least in promoting cell cycle withdrawal and horizontal cell production. In the mature retina, this protein is detected at the inner nuclear layer of all vertebrate groups. We have made a neurochemical characterisation of Prox1(+) cell types in two differ...

journal_title：Experimental eye research

authors： Cid E,Santos-Ledo A,Parrilla-Monge M,Lillo C,Arévalo R,Lara JM,Aijón J,Velasco A

更新日期：2010-02-01 00:00:00

abstract：:Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...

journal_title：Experimental eye research

authors： Kumar A,Xu Y,Yang E,Wang Y,Du Y

更新日期：2019-12-01 00:00:00

abstract：:The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...

journal_title：Experimental eye research

authors： Abbasoğlu OE,Hoşal BM,Sener B,Erdemoğlu N,Gürsel E

更新日期：2001-02-01 00:00:00

abstract：:Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water solubl...

journal_title：Experimental eye research

authors： Nagaraj RH,Monnier VM

更新日期：1990-10-01 00:00:00

abstract：:The R49C mutation of alphaA-crystallin (alphaA-R49C) causes hereditary cataracts in humans; patients in a four-generation Caucasian family were found be heterozygous for this autosomal dominant mutation. We previously generated knock-in mouse models of this mutation and found that by 2 months of age, heterozygous muta...

journal_title：Experimental eye research

authors： Andley UP,Reilly MA

更新日期：2010-06-01 00:00:00

abstract：:A shift or displacement of the retinal blood vessels (RBVs) with neuroretinal rim thinning indicates the progression of glaucomatous optic neuropathy. In chronic open angle glaucoma, individuals with RBV positional shifts exhibit more rapid visual field loss than those without RBV shifts. The retinal vessels reportedl...

journal_title：Experimental eye research

authors： Kuroda A,Enomoto N,Ishida K,Shimazawa M,Noguchi T,Horai N,Onoe H,Hara H,Tomita G

更新日期：2017-09-01 00:00:00

abstract：:We investigated the pharmacological actions of hydrogen sulfide (H(2)S) using sodium hydrosulfide (NaHS) and sodium sulfide (Na(2)S) as donors on isolated porcine irides in the presence of tone induced by muscarinic receptor stimulation. Furthermore, we also investigated the mechanism of action of H(2)S in this smooth...

journal_title：Experimental eye research

authors： Monjok EM,Kulkarni KH,Kouamou G,McKoy M,Opere CA,Bongmba ON,Njie YF,Ohia SE

更新日期：2008-12-01 00:00:00

abstract：:Early stages of glaucoma and optic neuropathies are thought to show inner retina remodeling and functional changes of retinal ganglion cells (RGCs) before they die. To assess RGC functional plasticity, we investigated the contrast-gain control properties of the pattern electroretinogram (PERG), a sensitive measure of ...

journal_title：Experimental eye research

authors： Chou TH,Feuer WJ,Schwartz O,Rojas MJ,Roebber JK,Porciatti V

更新日期：2016-04-01 00:00:00

abstract：:Post-translational modifications and/or structural changes induced by modifications are likely causes of the decrease in crystallin solubility associated with aging and the development of cataract. Characterization of human lens crystallins by mass spectrometry has demonstrated that betaB2-crystallin undergoes less mo...

journal_title：Experimental eye research

authors： Zhang Z,David LL,Smith DL,Smith JB

更新日期：2001-08-01 00:00:00

abstract：:Diabetic retinopathy is a complication of diabetes and a leading cause of vision loss among working-age adults. To assess whether the Wistar rat with Streptozotocin (STZ)-induced diabetes is a suitable animal model of human proliferative diabetic retinopathy we evaluated the vascular changes to assess the diabetic ret...

journal_title：Experimental eye research

authors： Naderi A,Zahed R,Aghajanpour L,Amoli FA,Lashay A

更新日期：2019-07-01 00:00:00

abstract：:γ-secretase inhibitor has been shown to promote intestinal goblet cell differentiation. We now demonstrated that the in vitro addition of γ-secretase inhibitor in the culture of human conjunctival epithelial cells significantly promoted the differentiation of conjunctival goblet cells with typical droplet-like phenoty...

journal_title：Experimental eye research

authors： Tian L,Qu M,Wang Y,Duan H,Di G,Xie L,Zhou Q

更新日期：2014-06-01 00:00:00

abstract：:An attachment to (Haag-Streit and Topcon) slit-lamps is described. This permits projection of a patterned target into the eye and the visual or photographic analysis of the details of the image reflected from the posterior surface of the crystalline lens. A numerical assessment can then be made of the optical quality ...

journal_title：Experimental eye research

authors： Weale R

更新日期：1992-09-01 00:00:00

abstract：:The purpose of this study was to examine the effects of myopia-inducing and myopia recovery conditions on the scleral biomechanics of enucleated eyes of young chicks. Enucleated eyes from 5-day old chicks, with fiducial markers attached at 5 locations on the external sclera, were placed in a custom-built chamber fille...

journal_title：Experimental eye research

authors： Lewis JA,Garcia MB,Rani L,Wildsoet CF

更新日期：2014-10-01 00:00:00

abstract：:The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...

journal_title：Experimental eye research

authors： Betz AL,Bowman PD,Goldstein GW

更新日期：1983-02-01 00:00:00

abstract：:Although it is important to investigate the effects of normal aging on the ocular circulation, few studies have examined the effects of aging on the retinal microcirculation in healthy subjects. The purpose of this study was to evaluate the effects of aging on the retinal microcirculation in healthy men. Forty-five he...

journal_title：Experimental eye research

authors： Nagaoka T,Sato E,Takahashi A,Sogawa K,Yokota H,Yoshida A

更新日期：2009-12-01 00:00:00

abstract：:Several lines of evidence support the existence of a renin-angiotensin system (RAS) in the retina that is separated from the blood stream by the retinal pigment epithelium (RPE). Under physiological conditions, increased activity of intraretinal RAS regulates neuronal activity of the retina but patho-physiologically p...

journal_title：Experimental eye research

authors： Martins JR,Reichhart N,Kociok N,Stindl J,Foeckler R,Lachmann P,Todorov V,Castrop H,Strauß O

更新日期：2019-12-01 00:00:00

abstract：:Myopia is the most common form of refractive eye disease, and the prevalence is increasing rapidly worldwide. However, the key metabolic alterations in individuals with high myopia are not understood clearly, and serum biomarkers remain to be determined. The objectives of this study were to identify serum biomarkers a...

journal_title：Experimental eye research

authors： Dai L,Yang W,Qin X,Li Y,Cao H,Zhou C,Wang Y

更新日期：2019-09-01 00:00:00

abstract：:The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...

journal_title：Experimental eye research

authors： Whiting RE,Pearce JW,Castaner LJ,Jensen CA,Katz RJ,Gilliam DH,Katz ML

更新日期：2015-05-01 00:00:00

abstract：:Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...

journal_title：Experimental eye research

authors： Wu W,Tholozan FM,Goldberg MW,Bowen L,Wu J,Quinlan RA

更新日期：2014-03-01 00:00:00