Abstract:
:Calf lens alpha-crystallin was isolated and the lysine residues were extensively modified with a variety of chemical agents. The effect of these modifications on elastase inhibitor activity, apparent molecular size, antibody reactivity and solubility were determined. The addition of either a methyl group or a threose residue did not alter the charge on the lysine residues and had little or no effect on either inhibitor activity or apparent molecular size. The introduction of a negative charge by either carboxymethylation or citraconylation caused a marked decrease in size and an almost complete loss of inhibitor activity. The introduction of a hydrophobic residue by reaction with either a trinitrobenzenesulfonic acid or Bolton Hunter reagent caused a slight increase in size, but a 70% increase in elastase inhibitor activity. Reaction with fluorescamine resulted in the dissociation of alpha-crystallin in a 200-kDa species, yet caused a two to four-fold increase in elastase inhibitor activity, which was similar to the activity of the water-insoluble fraction isolated from aged human lens and cataract. Several of these modified alpha-crystallins were compared for reactivity with a polyclonal alpha-crystallin antiserum using a quantitative slot blot assay. Charge neutral modifications resulted in a two to three-fold loss of antibody recognition, whereas the other preparations showed an almost complete loss of antigenic activity. None of the modifications caused the alpha-crystallin to precipitate at higher salt concentrations (0.3 M) with the exception of threose which caused a 30% decrease in soluble protein.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Ortwerth BJ,Olesen PR,Sharma KK,Prabhakaram Mdoi
10.1006/exer.1993.1015subject
Has Abstractpub_date
1993-01-01 00:00:00pages
107-14issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71015-8journal_volume
56pub_type
杂志文章abstract::Phosducin is a retinal and pineal phosphoprotein assumed to play an important role in visual phototransduction. Phosducin is also a uveitopathogenic retinal antigen, but its potency has been reported to be mild. During the course of studies aimed at identifying uveitopathogenic sites in phosducin, we found that rat ph...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0379
更新日期:1997-11-01 00:00:00
abstract::Bombina variegata 8 (Bv8), also known as prokineticin-2 (PK-2), is a potent pro-angiogenic factor. However, its role in retinal neovascularization (RNV) remains unknown. In this study, we explored the role of Bv8 in the pathogenesis of RNV. We found that the expression of Bv8 was significantly increased in two differe...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
abstract::In order to develop effective optical treatment strategies for myopia, it is important to understand how visual experience influences refractive development. Beginning with the discovery of the phenomenon of form deprivation myopia, research involving many animal species has demonstrated that refractive development is...
journal_title:Experimental eye research
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更新日期:2013-09-01 00:00:00
abstract::Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90059-6
更新日期:1989-06-01 00:00:00
abstract::Fiber cells of the ocular lens are arranged in a series of concentric shells. New growth shells are added continuously to the lens surface and, as a consequence, the preexisting shells are buried. To focus light, the refractive index of the lens cytoplasm must exceed that of the surrounding aqueous and vitreous humors...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.03.009
更新日期:2017-03-01 00:00:00
abstract::This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1047
更新日期:1994-05-01 00:00:00
abstract::Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1109
更新日期:1993-08-01 00:00:00
abstract::Erythropoietin (EPO) plays a critical role in the vascular system and exhibits angiogenic activity in endothelial cells (ECs) such as stimulation of cell proliferation, migration and tube formation in vitro. EPO is the major regulator of cell proliferation and differentiation of erythroid precursors and there by preve...
journal_title:Experimental eye research
pub_type: 杂志文章,收录出版
doi:10.1016/j.exer.2010.03.005
更新日期:2010-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80051-x
更新日期:1995-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.021
更新日期:2007-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0883
更新日期:2000-09-01 00:00:00
abstract::We developed an approach to generate a three-dimensional map that facilitates the assessment of epithelial nerve density in different corneal areas to define aging and gender influence on human corneal nerve architecture. Twenty-eight fresh human eyes from 14 donors of different ages were studied. Corneal nerves were ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.07.007
更新日期:2010-10-01 00:00:00
abstract::The purpose of this study was to examine the localization and relative levels of vascular endothelial growth factor (VEGF; an angiogenic factor) and pigment epithelium-derived factor (PEDF; an antiangiogenic factor) in aged human choroid and to determine if the localization or their relative levels changed in age-rela...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.05.007
更新日期:2006-01-01 00:00:00
abstract::Age-related macular degeneration (AMD) is the major cause of blindness for people over 60. In the "wet" form of AMD compounds targeting growth factor signaling pathways such as VEGF have been a major focus for therapeutic interventions. In a previously developed rat model of CNV, we utilized two receptor tyrosine kina...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.022
更新日期:2007-05-01 00:00:00
abstract::Previous studies have shown that corneal endothelial cells contain mRNA and protein of various growth factors. However, the role of these endogenous growth factors in corneal endothelial wound healing is not fully elucidated. In the present study, we investigated the role of endogenous factors and several growth facto...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0918
更新日期:2000-12-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/s0014-4835(03)00175-1
更新日期:2003-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2015.05.024
更新日期:2015-12-01 00:00:00
abstract::Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with norm...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(86)80023-9
更新日期:1986-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.04.009
更新日期:2017-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.1189
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abstract::Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is cap...
journal_title:Experimental eye research
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更新日期:1989-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.09.008
更新日期:2016-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.05.013
更新日期:2016-07-01 00:00:00
abstract::A technology has recently been developed that allows for the rapid transduction of full-length functionally active proteins into intact tissue through intravenous injection and into cultured cells. This technology involves the fusion of an 11 amino acid sequence of the HIV TAT protein to the protein of interest. In th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.12.010
更新日期:2004-05-01 00:00:00
abstract::We sought to determine the effects of exogenous epidermal growth factor (EGF), heparin-binding EGF (HB-EGF), transforming growth factor alpha (TGF-alpha), single-chain precursor hepatocyte growth factor (SC-HGF), double-chain mature HGF (DC-HGF), and keratinocyte growth factor (KGF) on proliferation, motility, and dif...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1152
更新日期:1994-12-01 00:00:00
abstract::The lysosomal proteases cathepsin B and dipeptidyl peptidase II (DPP II) were identified biochemically in the supernatant fraction of rat and guinea-pig lens homogenates, using as substrates CBZ-Arg-Arg-4-methylcoumarin-7-amide (AMC) (5 microM, pH 6.0, 37 degrees C for cathepsin B, and Lys-Ala-AMC or Lys-Pro-AMC (5 mi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80038-6
更新日期:1987-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0286
更新日期:1997-07-01 00:00:00
abstract::The causal mechanism of light damage in the albino rat retina has been investigated. Male and female albino Wistar rats weighing 200-300 g and previously maintained in normal room lighting of approximately 15 hr light (0.85 +/- 0.05 X 10(-4) W cm-2) per 9 hr dark cycle were simultaneously exposed continuously to unifo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90012-6
更新日期:1986-01-01 00:00:00
abstract::Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title:Experimental eye research
pub_type: 信件
doi:10.1016/j.exer.2009.03.024
更新日期:2009-09-01 00:00:00