Abstract:
:We sought to determine the effects of exogenous epidermal growth factor (EGF), heparin-binding EGF (HB-EGF), transforming growth factor alpha (TGF-alpha), single-chain precursor hepatocyte growth factor (SC-HGF), double-chain mature HGF (DC-HGF), and keratinocyte growth factor (KGF) on proliferation, motility, and differentiation of first passage cultures of human corneal epithelial cells in serum-free chemically defined medium. The effect of EGF, HB-EGF, TGF-alpha, SC-HGF, DC-HGF, KGF or combinations of the growth factors on proliferation was measured by counting cells present after 3 weeks of culture and by immunostaining for the cell-cycle-specific nuclear proliferation antigen Ki-67. The effect of the factors on epithelial cell motility was assessed by morphometric analysis of photographs of cells migrating from confluent islands of cells. The effect of growth factors on differentiation of epithelial cells were determined by immunostaining epithelial cell islands for the keratin K3 and by Western blotting for keratin K3. EGF, alone or in combination with KGF and SC-HGF, significantly stimulated motility of epithelial cells at the periphery of confluent islands of cells and induced an elongated cell morphology. TGF-alpha, HB-EGF and DC-HGF produced motility effects similar to EGF. There was diminished proliferation of the migrating cells in response to EGF, HB-EGF, TGF-alpha or DC-HGF, while non-migrating epithelial cells in the center of confluent islands continued to proliferate in response to the growth factors. EGF, HB-EGF, TGF alpha or DC-HGF inhibited expression of the differentiation-related marker keratin K3 in epithelial cells, both at the edge and at the center of the islands. KGF stimulated proliferation of corneal epithelial cells at low density and in confluent islands of cells. KGF did not affect expression of keratin K3 or migration of epithelial cells. SC-HGF had no effect on corneal epithelial cells. These results indicate that the effects of EGF, HB-EGF, TGF-alpha and DC-HGF on corneal epithelial cell proliferation, motility and differentiation vary from those of KGF and SC-HGF. EGF, HB-EGF, TGF-alpha and DC-HGF induced changes in epithelial cell morphology and motility in cells plated at low cell density or in cells located at the edge of a confluent island. Thus, these effects appear to be dependent on the extent of cell-cell contact. The inhibitory effect of EGF, HB-EGF, TGF-alpha or DC-HGF on corneal epithelial cell differentiation, however, is independent of cell density.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Wilson SE,He YG,Weng J,Zieske JD,Jester JV,Schultz GSdoi
10.1006/exer.1994.1152subject
Has Abstractpub_date
1994-12-01 00:00:00pages
665-78issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71152-3journal_volume
59pub_type
杂志文章abstract::A serum-free culture of chicken retinal pigmented epithelial cells has been established in order to analyse how cell-substrate interactions or environmental factors affect the process of transdifferentiation into lens cells from pigmented epithelial cells. The serum-free culture medium for chicken pigmented epithelial...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90190-4
更新日期:1992-08-01 00:00:00
abstract::Reactive nitrogen species, in particular, peroxynitrite (ONOO(-)) have been proposed to play an important role in the pathogenesis of endotoxin-induced uveitis (EIU). Tyrosine nitration by ONOO(-) has been shown in other model systems to inhibit the activity of the superoxide anion quenching enyzme, manganese superoxi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.1141
更新日期:2002-04-01 00:00:00
abstract::This study presents a computational model of the growth of the normal human lens and the induction of spoke-like cortical cataract in the aging lens. The anterior lens is modelled as a 2-D disk with a circumferential germinative zone. Lens cortical fibre cells in the same generation cover the surface in three identica...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0883
更新日期:2000-09-01 00:00:00
abstract::Human trabecular-meshwork (HTM) cells in culture convert arachidonic acid to two products: PGE2 and 6-keto PGF1 alpha. Prostaglandin PGE2 was the major product of arachidonic-acid metabolism. The synthesis and release of PGE2 and 6-keto PGF1 alpha was inhibited by a 15-min pre-treatment with indomethacin (5 X 10(-6) M...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90085-0
更新日期:1986-12-01 00:00:00
abstract::Research over the past two decades has shown that the growth of young eyes is guided by vision. If near- or far-sightedness is artificially imposed by spectacle lenses, eyes of primates and chicks compensate by changing their rate of elongation, thereby growing back to the pre-lens optical condition. Little is known a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0813
更新日期:2000-04-01 00:00:00
abstract::The combined effects of adenosine and acetylcholine on the intracellular free-Ca2+ concentration in nonpigmented epithelial cells of the rabbit ciliary body were investigated using fura-2 fluorescence-ratio imaging. Acetylcholine (10 microM) by itself produced a modest increase in [Ca2+]i. Acetylcholine in combination...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0194
更新日期:1997-02-01 00:00:00
abstract::Various concentrations of three dopamine antagonists (fluphenazine, haloperidol and sulpiride) with different affinities to the receptor subtypes were applied in order to test their effect on responses from outer (isolated PIII-component of the ERG), middle (b-wave) and inner (optic nerve response, ONR) retinal layers...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90038-g
更新日期:1991-04-01 00:00:00
abstract::Retinal lesions in the posterior pole of laboratory mice occur due to native, developmental abnormalities or as a consequence of environmental or experimental conditions. In this study, we investigated the rate and extent of retinal lesions as a result of prolonged ocular exposure following general anesthesia. Followi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.05.012
更新日期:2019-08-01 00:00:00
abstract::This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2003.11.016
更新日期:2004-04-01 00:00:00
abstract::Macular corneal dystrophy (MCD) is an autosomal recessive disease characterized by abnormal deposition of glycosaminoglycans in corneal stroma, keratocytes, Descemet's membrane and corneal endothelium. According to the presence and distribution of sulfated keratan sulfate (KS)-epitopes in serum and cornea (using mAb 5...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1080
更新日期:2001-11-01 00:00:00
abstract::Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.02.020
更新日期:2019-05-01 00:00:00
abstract::Interleukin (IL)-1alpha and beta are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, w...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.02.001
更新日期:2008-05-01 00:00:00
abstract::The Otx2 gene encodes a transcription factor essential for the normal development of brain, cerebellum, pineal gland, and eye. In the retina, Otx2 has essential functions from early embryogenesis to adulthood. As soon as the optic vesicle is formed, the gene is required for retinal pigment epithelium specification. Ot...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.03.007
更新日期:2013-06-01 00:00:00
abstract::Incorporated in the luminal glycocalyx of vascular endothelia (EC) are negatively charged microdomains (anionic sites). These sites are considered functionally important (a) in their interaction with circulating blood constituents, and (b) as a determinant of vascular permeability. The molecular composition of these E...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80134-4
更新日期:1995-10-01 00:00:00
abstract::Longitudinal (T1) magnetic relaxation times for the major phosphorus-containing metabolites present in the bovine and rabbit lens under organ culture conditions and in the bovine and rabbit globe have been determined. Significant differences in T1 for the major phosphorus metabolites in each case are observed, as well...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90045-9
更新日期:1984-10-01 00:00:00
abstract::The part of the light spectrum which is responsible for an increase of lenticular green autofluorescence upon white light exposure was determined for the rabbit. The increase of autofluorescence as a function of the light energy was measured, to assess a possible threshold energy for lens alterations. Thirteen rabbits...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1045
更新日期:1993-03-01 00:00:00
abstract::A lateral potential drop along the outer surface of the cornea could be measured at the edge of wounds made in the corneal epithelium of the isolated bovine eye when the cornea was covered by simulated tear film of modified Hanks' solution. These lateral fields (LFs) had an average magnitude of 42 +/- 1.4 mV mm-1 in t...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90164-n
更新日期:1992-06-01 00:00:00
abstract::We have previously shown that corticotropin-releasing hormone plays an important proinflammatory role in the induction of experimental autoimmune uveoretinitis. In this study, we examined the role of apoptosis in the destruction of the retina during experimental autoimmune uveoretinitis, and the role of corticotropin-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.0992
更新日期:2001-06-01 00:00:00
abstract::The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80042-3
更新日期:1988-06-01 00:00:00
abstract::Previous evidence has shown clearly that sugar cataract formation results from unusually high intracellular levels of polyol. Documentation of polyol-related histological changes in the cortical fiber cells of the equatorial zone has been extensive. However, little attention has been given to the early changes in the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90107-6
更新日期:1990-06-01 00:00:00
abstract::The purpose of this study was to assess the effect of taurine and apical potassium concentration modelling in vivo light evoked changes on the transepithelial potential (TEP) and the transepithelial resistance (TER) of isolated bovine retinal pigment epithelium (RPE). Isolated specimens of bovine non-tapetal RPE-Bruch...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.06.020
更新日期:2006-02-01 00:00:00
abstract::The nature and the extent of acute injury to corneal endothelial cells caused by exposure to ultrasound radiation were characterized, as well as the long-term reaction of these cells to this form of injury. It was found that the degree of lethal cell injury induced by ultrasound scaled with exposure intensity and dura...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0626
更新日期:1999-04-01 00:00:00
abstract::Understanding mechanisms of glaucomatous optic nerve damage is essential for developing effective therapies to augment conventional pressure-lowering treatments. This requires that we understand not only the physical forces in play, but the cellular responses that translate these forces into axonal injury. The former ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2010.08.005
更新日期:2011-08-01 00:00:00
abstract::Lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) activities were studied during corneal epithelial growth and differentiation in cell culture. LDH and G-6-PDH activities increased up to 60 and 150-fold, respectively, when corneal epithelial cells constituted a differentiated four to five lay...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1110
更新日期:2002-01-01 00:00:00
abstract::Exposure to hyposmotic solution causes release of ATP from lens cells via hemichannels. Because hemichannel opening feasibly could swamp the cells with calcium, we carried out studies to measure the magnitude of the increase in cytoplasmic calcium concentration caused by hemichannel opening. In studies on porcine lens...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.012
更新日期:2015-03-01 00:00:00
abstract::Axial-length and refraction measurements are presented for the lid-sutured rhesus monkey at 6-month intervals during the first 1.5 years of life. The experimental data are fitted to an exponential growth theory allowing the calculation of time constants, apparent time origins, and correlation coefficients for 10 eyes ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90043-6
更新日期:1986-06-01 00:00:00
abstract::The interphotoreceptor matrix (IPM) is a highly organized structure with interconnected domains surrounding cone and rod photoreceptor cells and extends throughout the subretinal space. Based on known roles of the extracellular matrix in other tissues, the IPM is thought to have several prominent functions including s...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2015.02.017
更新日期:2015-04-01 00:00:00
abstract::Racemisation of amino acids is one of the most abundant modifications in long-lived proteins. In this study racemisation of Asp 58 in the small heat shock protein, αA crystallin, was investigated. In normal human lenses, levels of l-isoAsp, d-isoAsp and d-Asp increased with age, such that by age 70 they accounted for ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.10.013
更新日期:2013-01-01 00:00:00
abstract::We have reported that the aspartyl (Asp)-151 residue in alphaA-crystallin in human eye lens was inverted to the D-isomer and isomerized to beta-Asp residue with age. We report here that ultraviolet (UV)-B irradiation induces the racemization and isomerization of the Asp-151 residue of alphaA-crystallin from lenses of ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0315
更新日期:1997-07-01 00:00:00
abstract::As a result of longitudinal chromatic aberration (LCA), longer wavelengths are blurred when shorter wavelengths are in focus, and vice versa. As a result, LCA affects the color and temporal aspects of the retinal image with hyperopic defocus. In this experiment, we investigated how the sensitivity to temporal color co...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108331
更新日期:2021-01-01 00:00:00