Abstract：
:The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinealocyte membranes. All-trans-retinal, but not retinol or retinoic acid, incubated with pinealocyte homogenates prior to reduction, resulted in a loss of radioactivity from the 30 kDa region of the gel. The radioactivity was also displaced by NH2OH in the dark. Incubation of cultured cells or homogenates with retinoyl fluoride, an acylating agent specific for the retinal binding site of opsins, also displaced radioactivity from the protein. Furthermore, retinoyl fluoride, added to chick pineal cells in culture, blocked the suppressive effect of light on melatonin output by these cells. Taken together these results raise the possibility that the 30 kDa protein mediates photosensitivity in the chick pineal.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Wallingford JC,Zatz Mdoi
10.1016/s0014-4835(88)80042-3subject
Has Abstractpub_date
1988-06-01 00:00:00pages
909-18issue
6eissn
0014-4835issn
1096-0007journal_volume
46pub_type
杂志文章abstract：:This study presents a computational model of the growth of the normal human lens and the induction of spoke-like cortical cataract in the aging lens. The anterior lens is modelled as a 2-D disk with a circumferential germinative zone. Lens cortical fibre cells in the same generation cover the surface in three identica...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0883
更新日期：2000-09-01 00:00:00
abstract：:Contact inhibition is an important mechanism for maintaining corneal endothelium in a non-replicative state. Protein tyrosine phosphatases (PTPs) play a role in regulating the integrity of cell-cell contacts, differentiation, and growth. In this study, we aimed to evaluate whether phosphatases are involved in the main...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.03.015
更新日期：2005-11-01 00:00:00
abstract：:The purpose of this study was to evaluate the inhibitory activity of the heparan sulfate suleparoide on vascular cell growth in vitro and angiogenesis in vivo. Human HUV-EC-C endothelial cell proliferation and microvessel sprouting from cultured rat aortic rings were assayed by the bioreduction of 3-[4,5-dimethylthiaz...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0512
更新日期：1998-08-01 00:00:00
abstract：:Utilizing cultured lenses from normal and homozygous glutathione peroxidase (GSHPx-1) knockout mice and inhibitors for GSSG Reductase (GSSG Red), 1,3-bis(2-chlorethyl)-1-nitrosourea (BCNU) and catalase (Cat), 3-aminotriazole (3-AT), the ability to degrade H2O2 was examined at two H2O2 concentrations, 300 microM and 80...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0250
更新日期：1997-03-01 00:00:00
abstract：:Beige adipocytes and brown adipocytes can generate heat by using mitochondrial uncoupling protein 1 (Ucp1), a thermogenic protein. Browning/beiging is the emergence of beige adipocytes in white adipose tissues (WAT) for cold acclimatization. Here we show the existence of brown/beige adipocytes in retro-orbital WAT in ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.03.021
更新日期：2019-07-01 00:00:00
abstract：:The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.02.012
更新日期：2015-05-01 00:00:00
abstract：:The extracellular matrix (ECM) plays a significant role in cellular and retinal health. The study of retinal tyrosine-sulfated proteins is an important first step toward understanding the role of ECM in retinal health and diseases. These secreted proteins are members of the retinal ECM. Tyrosine sulfation was shown to...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2014.07.007
更新日期：2015-04-01 00:00:00
abstract：:The purpose of this study was to investigate the roles of endothelium-derived factors in the retinal arteriolar responses to acute severe elevation in systemic blood pressure (BP) in cats. Acute elevation of mean arterial BP by 60% for 5 min was achieved by inflating a balloon-tipped catheter in the descending aorta. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.08.007
更新日期：2012-10-01 00:00:00
abstract：:The results reported here clearly document changes in the properties and the organization of fiber-cell membrane lipids that occur with age, based on electron paramagnetic resonance (EPR) analysis of lens membranes of clear lenses from donors of age groups from 0 to 20, 21 to 40, and 61 to 80 years. The physical prope...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.018
更新日期：2015-03-01 00:00:00
abstract：:Deficient cGMP-phosphodiesterase (cGMP-PDE) activity results in elevated levels of cGMP in the rd retina before any pathological signs are observed. Since the enzyme is present in rd retina, although it is barely activated by light, we determined whether its synthesis starts at the same time as that of cGMP-PDE in nor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80026-5
更新日期：1988-03-01 00:00:00
abstract：:Sodium hyaluronates with molecular weights of 18000, 500000 and 4 x 10(6), labelled with either 3H or 14C, were injected into the anterior chamber or into the centre of the vitreous body of rabbits and their rates of disappearance were followed. The out-flow from the anterior chamber in anaesthetized animals was virtu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90043-x
更新日期：1983-04-01 00:00:00
abstract：:Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.06.022
更新日期：2010-09-01 00:00:00
abstract：BACKGROUND:Indirect traumatic optic neuropathy (ITON) is a major cause of permanent loss of vision after blunt head trauma. Neuroinflammation plays a crucial role in neurodegenerative diseases. The present study concentrated on JNK/c-Jun-driven NLRP3 inflammasome activation in microglia during the degeneration of retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108335
更新日期：2021-01-01 00:00:00
abstract：:This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.11.016
更新日期：2004-04-01 00:00:00
abstract：:Antibiotics such as fluoroquinolones (FQLs) are commonly used to treat ocular infections but are also known to cause dermal melanocyte toxicity. The release of dispersed pigments from the iris into the aqueous humor has been considered a possible ocular side effect of the systemic administration of FQLs such as Moxifl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.006
更新日期：2017-09-01 00:00:00
abstract：:Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90183-0
更新日期：1984-06-01 00:00:00
abstract：:miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.11.006
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107826
更新日期：2019-12-01 00:00:00
abstract：:Previous studies in the laboratory have shown that platelet-activating factor (PAF), a potent inflammatory mediator that accumulates rapidly in the cornea after an injury, stimulates the expression of urokinase (uPA) and matrix metalloproteinase-1 (MMP-1) and -9 (MMP-9). Tissue inhibitors of MMPs (TIMPs) and plasminog...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1135
更新日期：2002-03-01 00:00:00
abstract：:This study aimed to assess the cytotoxic effect of low molecular weight components (LMWC) and conventional silicone oils (SOs) 1000 cSt with different degree of purification (raw, intermediate, and purified) using in vitro cytotoxicity tests. Direct contact cytotoxicity tests were performed in BALB 3T3 and human retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108018
更新日期：2020-05-01 00:00:00
abstract：:Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotec...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.022
更新日期：2011-11-01 00:00:00
abstract：:The mass receptor potential of the excised, superfused retina of the bullfrog was studied. Photoreceptor responses were isolated by addition of sodium aspartate to the Ringer solutions. Responses of the cones were monitored independently from responses of the rods by employing a two-flash method of stimulation which t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90007-1
更新日期：1984-08-01 00:00:00
abstract：:Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.02.007
更新日期：2019-04-01 00:00:00
abstract：:Thirty-one trabeculectomy specimens of patients suffering from primary open-angle glaucoma (POAG) who had received no, or only minor, medication prior to surgery were ultrastructurally and quantitatively analysed. Most of the specimens revealed thickened trabeculae, increased amounts of plaque-material deposited withi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1085
更新日期：1993-06-01 00:00:00
abstract：:Childhood glaucoma is an important cause of blindness world-wide. Eleven genes are currently known to cause inherited forms of glaucoma with onset before age 20. While all the early-onset glaucoma genes cause severe disease, considerable phenotypic variability is observed among mutations carriers. In particular, FOXC1...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107893
更新日期：2020-01-01 00:00:00
abstract：:Reactive astrocytes in glaucomatous optic nerve changes are characterized by an increased expression of alphaB-crystallin and transforming growth factor-beta (TGF-beta). In the pathogenesis of glaucomatous optic nerve damage, ischemia/reperfusion injury may play an important role. The goal of the present study was to ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.12.008
更新日期：2007-04-01 00:00:00
abstract：:A small percentage of retinal ganglion cells (RGCs) express melanopsin and are intrinsically photosensitive (ipRGCs). Whether light can affect the development of ipRGCs is not clear. In the rat retina, we found constant light exposure during the first postnatal week significantly increased the number of melanopsin imm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.03.011
更新日期：2013-06-01 00:00:00
abstract：:Omental cells (OCs) are shown to help wound healing. The purpose of this study is to investigate if OCs improve cornea repair after alkali injury by subconjunctival injection of activated OCs in rats. Forty eight hours after limbal corneal alkali injury, fresh isolated OCs were injected subconjunctivally into the reci...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.02.014
更新日期：2014-04-01 00:00:00
abstract：:Neuroretinal diseases are the predominant cause of irreversible blindness worldwide, mainly due to photoreceptor loss. Currently, there are no radical treatments to fully reverse the degeneration or even stop the disease progression. Thus, it is urgent to develop new biological therapeutics for these diseases on the c...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.108283
更新日期：2021-01-01 00:00:00
abstract：:Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stag...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108195
更新日期：2020-10-01 00:00:00
