Abstract:
:Lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) activities were studied during corneal epithelial growth and differentiation in cell culture. LDH and G-6-PDH activities increased up to 60 and 150-fold, respectively, when corneal epithelial cells constituted a differentiated four to five layered epithelium; these increases showed a similar time-course to the expression of K3 keratin. Immunostaining experiments showed that in growing colonies, LDH staining is stronger in those cells that are K3 positive; in contrast, in confluent four to five layered epithelia LDH and K3 were located in all cell layers, similar to the pattern found in frozen sections from rabbit central cornea. During growth and differentiation, the LDH isoenzyme set from corneal epithelial cells did not change; and it was different from those observed in cultured conjunctival, esophageal and epidermal cells. The augment in LDH activity was due to a 25-fold increase in the LDH-H mRNA and a 12-fold augment in LDH-M mRNA. A computer-assisted search led to identify AP2 and Sp1 binding sites in the LDH and G-6-PDH promoters, suggesting that their expression might share common regulatory mechanisms with the regulation of the differentiation-linked keratins. It is proposed that LDH may be an early marker of corneal epithelial differentiation, and its isozyme pattern could be distinctive from other epithelial cell lineages.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Hernández-Quintero M,García-Villegas R,Castro-Muñozledo Fdoi
10.1006/exer.2001.1110keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
71-82issue
1eissn
0014-4835issn
1096-0007pii
S0014483501911108journal_volume
74pub_type
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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更新日期:2000-08-01 00:00:00
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更新日期:1999-01-01 00:00:00
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更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2004-02-01 00:00:00
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doi:10.1016/j.exer.2005.01.019
更新日期:2005-08-01 00:00:00
abstract::We have determined the pattern of RNA labeling (uridine incorporation) in the normal retina of the domestic cat. One eye in each of eight cats was labeled by injecting [3H]uridine into the vitreous cavity. Two of the labeled eyes had the lens and vitreous removed 10 days before labeling. Three additional animals recei...
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