Abstract：
:The causal mechanism of light damage in the albino rat retina has been investigated. Male and female albino Wistar rats weighing 200-300 g and previously maintained in normal room lighting of approximately 15 hr light (0.85 +/- 0.05 X 10(-4) W cm-2) per 9 hr dark cycle were simultaneously exposed continuously to uniform fluorescent light flux of 3.01 +/- 0.5 X 10(-3) W cm-2 for a period of 6-18 hr. The animals were killed immediately after exposure and the retinas analysed for their contents of DNA, protein and lipids. There were significant losses of retinal DNA, protein, total lipid and polyunsaturated fatty acids, particularly docosahexaenoic acid. The losses were progressive with duration of exposure. There were also changes in phospholipid subclasses. These results could indicate altered photoreceptor-membrane viability secondary to continuous illumination. The ultimate effect would be the loss of photoreceptor cells with a concomitant loss of normal physiological function. Generally, gender was found to play no significant role, thus excluding possible endocrine interference. It is suggested that lipoperoxidative reactions could account for the observations made in this study. The danger of over-exposure to radiant flux in the tropics is inferred from these observations.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Akpalaba CO,Oraedu AC,Nwanze EAdoi
10.1016/0014-4835(86)90012-6subject
Has Abstractpub_date
1986-01-01 00:00:00pages
1-9issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(86)90012-6journal_volume
42pub_type
杂志文章abstract：:Recent epidemiological evidence in children indicates that time spent outdoors is protective against myopia. Studies in animal models (chick, macaque, tree shrew) have found that light levels (similar to being in the shade outdoors) that are mildly elevated compared to indoor levels, slow form-deprivation myopia and (...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.05.004
更新日期：2013-09-01 00:00:00
abstract：:Multidrug resistance associated protein (MRP) is a major family of efflux transporters involved in drug efflux leading to drug resistance. The objective of this study was to explore physical barriers for ocular drug absorption and to verify if the role of efflux transporters. MRP-2 is a major homologue of MRP family a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.08.015
更新日期：2007-01-01 00:00:00
abstract：:Utilizing cultured lenses from normal and homozygous glutathione peroxidase (GSHPx-1) knockout mice and inhibitors for GSSG Reductase (GSSG Red), 1,3-bis(2-chlorethyl)-1-nitrosourea (BCNU) and catalase (Cat), 3-aminotriazole (3-AT), the ability to degrade H2O2 was examined at two H2O2 concentrations, 300 microM and 80...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0250
更新日期：1997-03-01 00:00:00
abstract：:The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of the pathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters. We studied the hen to determine ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.017
更新日期：2006-10-01 00:00:00
abstract：:The protein phosphatase 2B inhibitor, FK506, is an immunomodulatory polypeptide that has neuroprotective properties, the mechanisms of which have not been elucidated. A possible mechanism may be phosphorylation-mediated regulation of glutamate transporter activity. In the present study, we investigated the effect of F...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2010.04.011
更新日期：2010-08-01 00:00:00
abstract：:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these le...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1068
更新日期：1994-06-01 00:00:00
abstract：:Glutamine, the end product formed by the glutamine synthetase (GS) reaction, inhibits retinal GS activity in the presence of Mn2+, but not in the presence of Mg2+. In the presence of Mg2+, Mn2+ itself inhibits retinal GS activity. Other compounds which inhibit retinal GS activity significantly are methionine sulfoximi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90109-5
更新日期：1985-01-01 00:00:00
abstract：PURPOSE:The feasibility of corneal reconstruction with cultured adult human corneal endothelial cells (HCEC) was examined in a nude rat model. METHODS:Endothelial cells were removed from the corneas of Lewis rats using a sterile cotton swab. Cultured adult HCEC labelled with a fluorescent marker chloromethyl-benzamido...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.05.001
更新日期：2004-08-01 00:00:00
abstract：:Anatomic and physiological barriers limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be a safe and effective means of treating various posterior segment diseases. Elimination of a compound from the vitreous chamber may depend on l...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00311-1
更新日期：2003-03-01 00:00:00
abstract：:Glucosyltransferase, an enzyme involved in the synthesis of the carbohydrate portion of basement membranes and collagens, and collagen content were determined in the retina of normal and streptozotocin diabetic rats. No significant difference in glucosyltransferase activity was found at 4 weeks of diabetes. However, t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80025-7
更新日期：1985-09-01 00:00:00
abstract：:Selective pericyte loss, the histological hallmark of early diabetic retinopathy (DR), enhances the breakdown of the blood-retinal barrier (BRB) in diabetes. However, the role of pericytes on BRB alteration in diabetes and the signaling pathways involved in their effects are currently unknown. To understand the role o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108043
更新日期：2020-06-01 00:00:00
abstract：:Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to und...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1034
更新日期：1993-03-01 00:00:00
abstract：:The nature and the extent of acute injury to corneal endothelial cells caused by exposure to ultrasound radiation were characterized, as well as the long-term reaction of these cells to this form of injury. It was found that the degree of lethal cell injury induced by ultrasound scaled with exposure intensity and dura...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0626
更新日期：1999-04-01 00:00:00
abstract：:The lysosomal proteases cathepsin B and dipeptidyl peptidase II (DPP II) were identified biochemically in the supernatant fraction of rat and guinea-pig lens homogenates, using as substrates CBZ-Arg-Arg-4-methylcoumarin-7-amide (AMC) (5 microM, pH 6.0, 37 degrees C for cathepsin B, and Lys-Ala-AMC or Lys-Pro-AMC (5 mi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80038-6
更新日期：1987-06-01 00:00:00
abstract：:We have examined the mercapturic acid pathway of the cataractous rabbit lens following induction by naphthalene as an oxidative foreign substance. 1,2-Naphthoquinone, which is formed in the eye from naphthalene diol and other naphthalene derivatives by a combination of enzymic and non-enzymic reactions, readily oxidiz...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90058-9
更新日期：1988-09-01 00:00:00
abstract：:Understanding how tissues and organs acquire and maintain an appropriate size and shape remains one of the most challenging areas in developmental biology. The eye lens represents an excellent system to provide insights into regulatory mechanisms because in addition to its relative simplicity in cellular composition (...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.04.009
更新日期：2017-03-01 00:00:00
abstract：:This study presents a computational model of the growth of the normal human lens and the induction of spoke-like cortical cataract in the aging lens. The anterior lens is modelled as a 2-D disk with a circumferential germinative zone. Lens cortical fibre cells in the same generation cover the surface in three identica...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0883
更新日期：2000-09-01 00:00:00
abstract：:We sequenced selected peptides of alligator rhodopsin that accounted for about half of the total protein. These sequences were confirmed when the total primary structure of alligator rhodopsin was deduced from the cDNA sequence. Differences in the amino-terminal region, compared to that of bovine rhodopsin, account fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80051-x
更新日期：1995-11-01 00:00:00
abstract：:This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.007
更新日期：2013-10-01 00:00:00
abstract：:Exposure to sulfur mustard (SM) may result in severe ocular injuries. While some of the eyes show a clinical resolution of the injury (defined as clinically non-impaired), part of the eyes develop irreversible late ocular pathologies (defined as clinically impaired) that may lead to corneal blindness. Understanding th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.011
更新日期：2019-07-01 00:00:00
abstract：:Mitochondrial damage and epigenetic modifications have been implicated in the pathogenesis of Age-related Macular Degeneration (AMD). This study was designed to investigate the effects of AMD/normal mitochondria on epigenetic regulation in human transmitochondrial retinal pigment epithelial (RPE) cells in vitro. Human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107701
更新日期：2019-12-01 00:00:00
abstract：:This study describes the phosphatic metabolite contribution of the porcine corneal stroma [by phosphorus-31 nuclear magnetic resonance (P-31 NMR)] and the corneal epithelium (by difference calculation) to the total phosphatic metabolic profile of the intact (i.e. whole, undissected) cornea. In order to provide such a ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90147-2
更新日期：1985-03-01 00:00:00
abstract：:The effects of metabolic inhibitors on the apical and basal membrane potentials were studied in the isolated retinal pigment epithelium of the lizard Gekko gekko. Adding dinitrophenol or cyanide or cooling the tissue to 15 degrees C first depolarized the apical membrane and then hyperpolarized the basal membrane. The ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90015-m
更新日期：1990-01-01 00:00:00
abstract：:The Raman microspectroscopic method was used to determine the local water and protein content in human lenses. In 18 lenses of varying age position-defined water/protein content measurements were carried out along the visual and the equatorial axis. A main characteristic of the human lens is its constant and relativel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90079-t
更新日期：1991-08-01 00:00:00
abstract：:The reversible phosphorylation of proteins plays essential roles in regulating various cellular events, and is regulated by the opposing actions of protein kinases and protein phosphatases. Protein kinases in the lens system have been well studied, but very little is known about lens protein phosphatases. Protein phos...
journal_title：Experimental eye research
pub_type： 更正并重新发布的文章,杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：:This review offers a contemporary history of the renin-angiotensin (RAS) and kallikrein-kinin (KKS) systems with emphasis on how these complex systems affect the eye. It describes the types of communication (cross-talk) between the two systems and evaluates their potential role in the development of diabetic retinopat...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.05.007
更新日期：2018-01-01 00:00:00
abstract：:This review describes our recent attempts to develop new therapeutic modalities for corneal endothelial disease using animal models including non-human primate model in which the proliferative ability of corneal endothelial cells is severely limited, as is the case in humans. First, we describe our attempt to develop ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2011.10.014
更新日期：2012-02-01 00:00:00
abstract：:This study tested the hypothesis that the beta-adrenergic antagonists betaxolol and timolol, cause retinal arteriolar vasodilatation in addition to their ability to reduce intraocular pressure (IOP), and compared their vasodilatory ability with that of a known Ca2+ channel entry blocker nimodipine in donor human and p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0495
更新日期：1998-07-01 00:00:00
abstract：:Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2004.06.011
更新日期：2004-09-01 00:00:00
abstract：:Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the pe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.08.011
更新日期：2017-11-01 00:00:00
