Abstract：
:The effects of metabolic inhibitors on the apical and basal membrane potentials were studied in the isolated retinal pigment epithelium of the lizard Gekko gekko. Adding dinitrophenol or cyanide or cooling the tissue to 15 degrees C first depolarized the apical membrane and then hyperpolarized the basal membrane. The basal hyperpolarization was accompanied by an apparent increase in basal resistance. These effects were fully reversible. Adding ouabain to inhibit specifically the apical Na(+)-K+ pump irreversibly depolarized the apical membrane but did not produce a basal membrane hyperpolarization. Dinitrophenol, cyanide and azide also reversibly inhibited a basal membrane response that was evoked by changing the apical potassium concentration. Ouabain did not inhibit this potassium-evoked basal response. These results suggest that metabolic inhibitors will be useful tools to study RPE basal membrane function.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Griff ERdoi
10.1016/0014-4835(90)90015-msubject
Has Abstractpub_date
1990-01-01 00:00:00pages
99-107issue
1eissn
0014-4835issn
1096-0007journal_volume
50pub_type
杂志文章abstract：:This study was undertaken to develop a feline model of corticosteroid-induced ocular hypertension. In the first experiment, eight cats were selected whose intraocular pressure (17 +/- 0.4 mmHg) was consistently below the mean baseline intraocular pressure of our colony (24 +/- 0.5) during the preceding 2 months. Unila...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80210-6
更新日期：1992-02-01 00:00:00
abstract：:The transport kinetics across the plasma-ocular barriers of labeled molecules including urea, D-glucose, 3-0-methyl-D-glucose, L-glucose, and sucrose were studied in adult RCS rats and compared to control albino Sprague-Dawleys (SD). Results indicate that substances that passively cross the plasma-aqueous and plasma-v...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90156-2
更新日期：1983-09-01 00:00:00
abstract：BACKGROUND:Indirect traumatic optic neuropathy (ITON) is a major cause of permanent loss of vision after blunt head trauma. Neuroinflammation plays a crucial role in neurodegenerative diseases. The present study concentrated on JNK/c-Jun-driven NLRP3 inflammasome activation in microglia during the degeneration of retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108335
更新日期：2021-01-01 00:00:00
abstract：:Prostaglandin E2 (PGE2) markedly reduces intraocular pressure (IOP) when applied topically and induces strong relaxation of pre-contracted isolated ciliary muscle through PGE2 receptor. Because the ciliary muscle relaxation reduces IOP by enhancing uveoscleral aqueous outflow, the ciliary muscle where the existence of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0829
更新日期：2000-05-01 00:00:00
abstract：:Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.06.022
更新日期：2010-09-01 00:00:00
abstract：:The retinal pigment epithelium (RPE) is essential for maintaining the health of the neural retina. RPE cell dysfunction plays a critical role in many common blinding diseases including age-related macular degeneration (AMD), diabetic retinopathy, retinal dystrophies. Mouse models of ocular disease are commonly used to...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.09.015
更新日期：2016-04-01 00:00:00
abstract：:Prostaglandins are widely used to lower intraocular pressure (IOP) as part of the treatment regimen for glaucoma. While FP and EP2 agonists are known to lower IOP, we investigated the ocular hypotensive activity and ocular drug distribution of PF-04475270, a novel EP4 agonist following topical administration in normot...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.05.002
更新日期：2009-11-01 00:00:00
abstract：:Lens development and transparency have been hypothesized to depend on intercellular gap junction channels, consisting of alpha3 (Cx46) and alpha8 (Cx50) connexin subunits, to transport metabolites, secondary messages and ions between lens cells. To evaluate this hypothesis, we have generated alpha3(-/-) alpha8(-/-) do...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.03.013
更新日期：2006-09-01 00:00:00
abstract：:Previous studies have shown that retinal damage induced by damaging light exposure is more severe in superior retina than inferior retina when measured along the vertical meridian of eyes. However, the extent of retinal damage over all retinal regions is not clear. Albino rats were exposed to bright light (5000 lux fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.06.002
更新日期：2008-09-01 00:00:00
abstract：:The retinal pigment epithelial cell has several important functions, one of which is the phagocytosis of photoreceptor outer segments which are discarded diurnally. We previously provided evidence in human retinal pigment epithelium that CD36, an 88 kDa integral membrane glycoprotein, participates in the phagocytosis ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0177
更新日期：1997-01-01 00:00:00
abstract：:The aim of this study was to optimize non-viral gene transfer conditions and investigate the effect of fibroblast growth factor-1 (FGF-1) gene transfer on human corneal endothelial cell (HCEC) proliferation. Five non-viral vectors (Lipofectin, DMRIE-C, DAC-30, Effectene, FuGene6) were used to transfect HCEC with plasm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.08.024
更新日期：2005-01-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
abstract：:Exfoliation syndrome (XFS) is an important risk factor for glaucoma (XFG) worldwide. LOXL1 variants are highly associated with XFS in most populations; however, the high frequency of risk alleles in normal individuals and the reversal of risk alleles in different ethnic populations suggest that other factors contribut...
journal_title：Experimental eye research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.exer.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：:Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0551
更新日期：1998-11-01 00:00:00
abstract：:Reactive nitrogen species, in particular, peroxynitrite (ONOO(-)) have been proposed to play an important role in the pathogenesis of endotoxin-induced uveitis (EIU). Tyrosine nitration by ONOO(-) has been shown in other model systems to inhibit the activity of the superoxide anion quenching enyzme, manganese superoxi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.1141
更新日期：2002-04-01 00:00:00
abstract：:The suggested common regulator of the eye lens crystallin genes is c-Maf. Maf responsive elements have been detected in a number of crystallin promoters including that of the rat betaB2-crystallin gene. The betaB2-crystallin gene is active in the post-natal lens and its mRNA reaches its maximal level in the rat lens 6...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1077
更新日期：2001-11-01 00:00:00
abstract：:(O-acyl) ω-hydroxy fatty acids (OAHFAs) are a recently found group of polar lipids in meibum. Since these lipids can potentially serve as a surfactant in the tear film lipid layer, the surface properties of a molecule of this lipid class was investigated and compared with a structurally related wax ester and a fatty a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.016
更新日期：2013-10-01 00:00:00
abstract：:Hypertension is an independent risk factor for diabetic retinopathy, yet anti-hypertensive medications such as blockade of angiotensin II do not completely protect against vision-threatening vascular disease. We hypothesized that the potent vasoactive factor, endothelin (ET), is up-regulated in diabetic retinopathy an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.06.025
更新日期：2018-11-01 00:00:00
abstract：:We wished to conduct a light and electron microscopic investigation of pigmentation within the trabecular meshwork of normals and primary open angle glaucoma (POAG) patients. In particular we wished to get a precise determination of whether there was a relationship between pigmentation and age. In addition we wanted t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.10.009
更新日期：2006-06-01 00:00:00
abstract：:A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.04.007
更新日期：2014-06-01 00:00:00
abstract：:Glaucoma is defined as a progressive optic neuropathy and is characterized by an irreversible loss of retinal ganglion cells. The main risk factor to develop glaucoma is an increased intraocular pressure (IOP). During the course of glaucoma structural changes in the optic nerve head (ONH) take place which lead to the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.08.014
更新日期：2016-01-01 00:00:00
abstract：:For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.03.027
更新日期：2018-07-01 00:00:00
abstract：:The phagocytosis of photoreceptor outer segments (OS) by the retinal pigment epithelium (RPE) is a receptor mediated process. A key component of this process is the receptor tyrosine kinase, Mer. RPE cells from the RCS rat, which lacks a functional mer gene, and do not express Mer protein, are able to bind OS, but are...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00158-1
更新日期：2003-09-01 00:00:00
abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:Mice carrying the rd mutation are affected with an autosomal recessive disease characterized by the total degeneration of retinal photoreceptor cells, which begins at postnatal day 8 and reaches completion by 3 wk of life. Biochemical studies have led to the proposal that a lesion in cGMP metabolism may be the cause o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80079-x
更新日期：1989-02-01 00:00:00
abstract：:Our previous studies in bovine eyes demonstrated that the structural correlate to the increase in outflow facility after either Rho-kinase inhibitor Y-27632 (Y27) treatment or washout appeared to be separation between the juxtacanalicular tissue (JCT) and inner wall (IW) of the aqueous plexus, the bovine equivalent of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.05.012
更新日期：2011-10-01 00:00:00
abstract：:Bombina variegata 8 (Bv8), also known as prokineticin-2 (PK-2), is a potent pro-angiogenic factor. However, its role in retinal neovascularization (RNV) remains unknown. In this study, we explored the role of Bv8 in the pathogenesis of RNV. We found that the expression of Bv8 was significantly increased in two differe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107993
更新日期：2020-04-01 00:00:00
abstract：:Adenosine, produced from the decomposition of adenosine triphosphate, is believed to provide protective effects during ischemia. On the other hand, adenosine metabolites may serve as precursors for oxygen free radical formation. The time course of formation of adenosine and its purine metabolites was studied during re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0391
更新日期：1997-12-01 00:00:00
abstract：:The Otx2 gene encodes a transcription factor essential for the normal development of brain, cerebellum, pineal gland, and eye. In the retina, Otx2 has essential functions from early embryogenesis to adulthood. As soon as the optic vesicle is formed, the gene is required for retinal pigment epithelium specification. Ot...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.03.007
更新日期：2013-06-01 00:00:00
abstract：:Rabbit immunoglobulin G (IgG) binds specifically to the human corneal epithelium. We investigated this phenomenon to identify the binding material in the tissue. Sections of human cornea were immunostained with negative-control grade rabbit IgG, F(ab')(2) (the antigen recognition fragment of IgG yielded by pepsin dige...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.01.006
更新日期：2004-06-01 00:00:00
