Abstract：
:The aim of this study was to optimize non-viral gene transfer conditions and investigate the effect of fibroblast growth factor-1 (FGF-1) gene transfer on human corneal endothelial cell (HCEC) proliferation. Five non-viral vectors (Lipofectin, DMRIE-C, DAC-30, Effectene, FuGene6) were used to transfect HCEC with plasmids coding for enhanced green fluorescent protein (EGFP) and FGF-1. Transfection efficiency and toxicity (n=6) were quantified and optimized using the EGFP construct by FACS-analysis. Using optimal conditions HCEC were transfected with the FGF-1 plasmid and cell proliferation as well as expression of FGF-1 were determined at days 4 and 7 by counting and western blotting, respectively. Lipofectin (17+/-2.02%) transfected HCEC more successfully than DMRIE-C (11+/-1.46%), Effectene (9+/-0.62%), FuGene (9+/-0.93%) and DAC-30 (7+/-0.59%). Toxicity of the lipids ranged from 2 to 4%. Optimal HCEC proliferation was achieved with DAC-30/FGF-1 (P<0.05), whereas all other vectors did not result in significantly increased cell proliferation. However, all of the transfected cells produced FGF-1 in different amounts as indicated by western blotting. Efficient and almost non-toxic transfer of the FGF-1 gene into HCEC can be successfully achieved by lipid-based techniques. Using optimal conditions significantly increased cell proliferation was independent on gene transfer efficiency. This may indicate that even a low transfection rate is sufficient to produce a concentration of FGF-1 that will have a stimulatory effect on HCECs.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Dannowski H,Bednarz J,Reszka R,Engelmann K,Pleyer Udoi
10.1016/j.exer.2004.08.024keywords:
subject
Has Abstractpub_date
2005-01-01 00:00:00pages
93-101issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(04)00236-2journal_volume
80pub_type
杂志文章abstract：:The overall importance of the peak or the mean serum concentrations as predictors of ocular drug penetration is unknown. To address this fundamental question with an agent which shows promise as adjunctive therapy in the treatment of endophthalmitis, we studied the penetration of ciprofloxacin into the aqueous and vit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0101
更新日期：1996-08-01 00:00:00
abstract：:There is currently no available method to efficiently deliver proteins across the plasma membrane of photoreceptor or retinal pigment epithelium (RPE) cells in vivo. Thus, current clinical application of recombinant proteins in ophthalmology is limited to the use of proteins that perform their biological function extr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.009
更新日期：2016-04-01 00:00:00
abstract：:A key model for examining the activity-dependent development of primary visual cortex (V1) involves the imbalance in activity between the two eyes induced by monocular deprivation (MD). MD early in life causes dramatic changes in the functional and structural organization of mammalian visual cortex. The molecular sign...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.12.016
更新日期：2013-04-01 00:00:00
abstract：:To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0429
更新日期：1998-03-01 00:00:00
abstract：:Exposure of the rabbit lens, in vitro, to 10(-3) M-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10(-4) or 10(-5) M-hydrogen peroxide. 10(-3) M-hydrogen peroxide was observ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90148-3
更新日期：1983-07-01 00:00:00
abstract：:Cultured bovine lens epithelial cells adapt to hypertonic sodium stress via an increase in Na(+)-myo-inositol cotransporter activity and accumulate myo-inositol. At least 12 hr of hypertonic exposure was necessary to enhance myo-inositol accumulation; and thereafter, uptake activity continued to increase throughout th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1083
更新日期：1994-07-01 00:00:00
abstract：:Anti-Vascular Endothelial Growth Factors (Anti-VEGF) agents have received recent interest as potential anti-fibrotic agents for their concurrent use with trabeculectomy. Preliminary cohort studies have revealed improved bleb morphology following trabeculectomy augmented with ranibizumab. The effects of this humanized ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.08.005
更新日期：2014-10-01 00:00:00
abstract：:This is a brief overview of multifaceted anatomical, experimental and clinical studies conducted by the author since 1955 on the optic nerve head circulation in health and disease. Conclusions, based on the accumulated information provided by these studies, are summarized. The studies on the pattern of blood supply of...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(05)80121-6
更新日期：1995-09-01 00:00:00
abstract：:There is increasing evidence that the macular pigment carotenoids, lutein and zeaxanthin, may play an important role in the prevention of age-related macular degeneration, cataract, and other blinding disorders. Although it is well known that the retina and lens are enriched in these carotenoids, relatively little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0954
更新日期：2001-03-01 00:00:00
abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107955
更新日期：2020-04-01 00:00:00
abstract：:In keratitis, keratocytes play a vital role by releasing inflammatory cytokines and expressing intercellular cell adhesion molecule-1(ICAM-1). GC31 is a peptide derived from thrombomodulin, an endogenous protein with potential anti-inflammation properties. We evaluated the protective effect of GC31 in LPS- or poly(I:C...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.017
更新日期：2017-11-01 00:00:00
abstract：:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1156
更新日期：1993-11-01 00:00:00
abstract：:We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23 degrees C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We foun...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90257-f
更新日期：1991-02-01 00:00:00
abstract：:A newly-developed fibre-optic sensor (wavelength of 670 nm) was used to measure the index gradient in 16 human lenses ranging in age from 38 to 78 years. Two of these lenses had nuclear cataract. The refractive index, calculated from the proportion of reflected light at the sensor/sample interface, appeared to increas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80097-1
更新日期：1995-04-01 00:00:00
abstract：:The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title：Experimental eye research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0014-4835(05)80019-3
更新日期：1995-12-01 00:00:00
abstract：:Retinal pigment epithelial cell dysfunction mediated by reactive oxygen intermediates has been suggested as a possible cause of age-related macular degeneration. To test the hypothesis that retinal pigment cells are susceptible to genetic damage mediated by reactive oxygen intermediates, retinal pigment epithelial cel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0661
更新日期：1999-06-01 00:00:00
abstract：:Understanding mechanisms of glaucomatous optic nerve damage is essential for developing effective therapies to augment conventional pressure-lowering treatments. This requires that we understand not only the physical forces in play, but the cellular responses that translate these forces into axonal injury. The former ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.08.005
更新日期：2011-08-01 00:00:00
abstract：:Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to und...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1034
更新日期：1993-03-01 00:00:00
abstract：:Childhood glaucoma is an important cause of blindness world-wide. Eleven genes are currently known to cause inherited forms of glaucoma with onset before age 20. While all the early-onset glaucoma genes cause severe disease, considerable phenotypic variability is observed among mutations carriers. In particular, FOXC1...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107893
更新日期：2020-01-01 00:00:00
abstract：:Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90059-6
更新日期：1989-06-01 00:00:00
abstract：:Studies using conventional electron microscopy describe the cytoplasm of lens fiber cells as having essentially an amorphous structure. We hypothesized that significant structural detail might have been lost as a result of projecting the entire thickness of the section (50-100 nm) onto a single plane (the "projection ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.11.029
更新日期：2009-03-01 00:00:00
abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1016/j.exer.2010.03.008. The duplicate article has therefore been withdrawn. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.07.012
更新日期：2011-07-29 00:00:00
abstract：:Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.11.001
更新日期：2018-02-01 00:00:00
abstract：:Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.01.001
更新日期：2017-02-01 00:00:00
abstract：:The cornea is a self-renewing tissue located at the front of the eye. Its transparency is essential for allowing light to focus onto the retina for visual perception. The continuous renewal of corneal epithelium is supported by limbal stem cells (LSCs) which are located in the border region between conjunctiva and cor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.09.008
更新日期：2016-11-01 00:00:00
abstract：:Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (L-isoproterenol greater than or equal to L-epinephrine greater than L-norepinephrine) and some prostagla...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80158-6
更新日期：1987-04-01 00:00:00
abstract：:The DBA/2 mouse has been used as a model for spontaneous secondary glaucoma. We attempted to determine the in vivo time course and spatial distribution of retinal ganglion cells (RGCs) undergoing apoptotic death in DBA/2 mice. Female DBA/2 mice, 3, 9-10, 12, 15, and 18 months of age, received intravitreal injections o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.08.009
更新日期：2007-01-01 00:00:00
abstract：:Multiple species of mucins are synthesized and secreted by corneal and conjunctival epithelial cells. These mucins are vital components of the tear film protecting the ocular surface from the external environment by providing a physical and chemical barrier. The release of mucins must be tightly regulated as both muci...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.10.005
更新日期：2004-02-01 00:00:00
abstract：:Previously, we demonstrated that the inwardly rectifying K(+) (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reve...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.05.010
更新日期：2008-09-01 00:00:00
abstract：:The identification of genes that modify pathological ocular phenotypes in mouse models may improve our understanding of disease mechanisms and lead to new treatment strategies. Here, we identify modifier loci affecting photoreceptor cell loss in homozygous Mfrp(rd6) mice, which exhibit a slowly progressive photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.020
更新日期：2014-01-01 00:00:00
