Abstract:
:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have attempted to prepare cultures suitable for measurements of ion and water flux. We have grown rabbit ciliary body epithelial cells on permeable supports, coated with several extracellular matrix proteins. We then examined the ability of these proteins to promote the growth of a differentiated layer of epithelial cells. Non-pigmented and pigmented cells formed sheets of contiguous cells when grown on a variety of support media. The most successful substrate was a permeable support produced by Falcon/Becton Dickinson coated with a mixture of collagen IV, laminin and heparan sulfate. Under these conditions, cultures could be maintained for several months, but pigmented cell cultures did not develop a measurable transepithelial resistance (TER), and the TER of non-pigmented cell cultures was typically only 20-30 omega cm2. Much higher TERs exceeding 200 omega cm2 could be measured from non-pigmented cell cultures 3-5 days after plating, but these high values were unstable. Examination of the cultures with electron microscopy revealed that the cells were partially differentiated with the formation of a basal lamina and intercellular junctions. Labelling with a specific monoclonal antibody marker for tight junction protein (ZO-1) suggested that non-pigmented cell cultures showed extensive tight junction formation. The low TER of the non-pigmented cell cultures appears therefore not to be due to the lack of tight junctions but rather to the presence of spaces between cells.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Cilluffo MC,Fain MJ,Fain GLdoi
10.1006/exer.1993.1156subject
Has Abstractpub_date
1993-11-01 00:00:00pages
513-26issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71156-5journal_volume
57pub_type
杂志文章abstract::Lactate dehydrogenase (LDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) activities were studied during corneal epithelial growth and differentiation in cell culture. LDH and G-6-PDH activities increased up to 60 and 150-fold, respectively, when corneal epithelial cells constituted a differentiated four to five lay...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90138-1
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journal_title:Experimental eye research
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更新日期:1983-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0661
更新日期:1999-06-01 00:00:00
abstract::Albino mice exposed to 1 X 10(-4) W cm-2 of UV radiation in the range 290-400 nm for 12 hr per day developed anterior lens opacities within 1-2 months after onset of the exposure. Histological changes demonstrated marked derangement of the lens epithelium. When the radiation was filtered with glass, eliminating radiat...
journal_title:Experimental eye research
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doi:10.1016/s0014-4835(85)80011-7
更新日期:1985-10-01 00:00:00
abstract::For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...
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pub_type: 杂志文章
doi:10.1016/j.exer.2018.03.027
更新日期:2018-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.10.020
更新日期:2014-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107822
更新日期:2019-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0101
更新日期:1996-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.004
更新日期:2006-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80042-3
更新日期:1988-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.10.008
更新日期:2014-11-01 00:00:00
abstract::To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0429
更新日期:1998-03-01 00:00:00
abstract::Specific [3H]-dexamethasone binding sites were demonstrated in bovine corneal cytosol using titration analyses. Specific glucocorticoid binding to corneal cytosol exhibited a single class of sites with apparent dissociation constants of 4-6 nM and 68-72 fmol binding sites mg-1 cytosolic protein. Association of steroid...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90188-x
更新日期:1984-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.12.013
更新日期:2016-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1057
更新日期:1994-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.11.013
更新日期:2006-07-01 00:00:00
abstract::Colchicine, a naturally occurring alkaloid, inhibits both fast and slow axoplasmic transport by prevention of microtubule assembly. We have examined the influence of colchicine on the neurogenically mediated acute inflammatory responses to topically administered nitrogen mustard and formaldehyde on the albino rabbit e...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90071-x
更新日期:1984-12-01 00:00:00
abstract::The results reported here clearly document changes in the properties and the organization of fiber-cell membrane lipids that occur with age, based on electron paramagnetic resonance (EPR) analysis of lens membranes of clear lenses from donors of age groups from 0 to 20, 21 to 40, and 61 to 80 years. The physical prope...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.018
更新日期:2015-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(85)80025-7
更新日期:1985-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1015
更新日期:1993-01-01 00:00:00
abstract::Thirty-one trabeculectomy specimens of patients suffering from primary open-angle glaucoma (POAG) who had received no, or only minor, medication prior to surgery were ultrastructurally and quantitatively analysed. Most of the specimens revealed thickened trabeculae, increased amounts of plaque-material deposited withi...
journal_title:Experimental eye research
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doi:10.1006/exer.1993.1085
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abstract::Corneal mycotic ulceration is predominantly due to Aspergillus and Fusarium solani infection in tropical countries. In this study, we examined the proteome profile of tear samples from A. flavus keratitis patients at various stages of infection. The proteome was profiled using 2D PAGE and the protein levels were quant...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107700
更新日期:2019-09-01 00:00:00
abstract::Tissue from the outflow system of six surgically enucleated aged eyes was used for an ultrastructural immunocytochemical study of the distribution of laminin and type IV collagen. The immunogold technique provided precise localization of laminin beneath lining endothelial cells of the inner wall of Schlemm's canal. La...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90054-x
更新日期:1990-12-01 00:00:00
abstract::Murine macrophages treated with TGF-beta2 are capable of inducing anterior chamber-associated immune deviation (ACAID), and these macrophages are characterized by impaired IL-12 production and CD40 expression, consequently failing to promote Th1 cell differentiation. In this study, we investigated whether human monocy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.05.005
更新日期:2006-10-01 00:00:00
abstract::Intraocular pressure (IOP) is an important risk factor for glaucoma, and the response of the ONH and surrounding tissues to elevated IOP are often investigated to better understand pathophysiology. In vivo structure including that of the optic nerve head (ONH) and surrounding tissue of the eye are often assessed using...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.01.025
更新日期:2018-04-01 00:00:00
abstract::The levels of alpha 1-proteinase inhibitor (alpha 1-antitrypsin) in keratoconus, normal human, and other diseased corneas were examined. Using an immunoperoxidase technique, the presence of this inhibitor was demonstrated in the epithelium, stroma and endothelium of all corneal sections. Compared with normal human con...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90044-u
更新日期:1990-05-01 00:00:00
abstract::Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90088-c
更新日期:1990-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.02.006
更新日期:2009-06-15 00:00:00
abstract::Purified retinal pigment epithelial cells of bovine eyes have been fractionated by a series of buffer and detergent extractions. The electropherogram of the buffer-insoluble, Triton X-100-soluble fraction (RPE-TS) exhibits a major polypeptide band of M(r) 65 kDa and a variety of minor components. Electrophoretically p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90008-g
更新日期:1992-12-01 00:00:00