Abstract:
:Corneal mycotic ulceration is predominantly due to Aspergillus and Fusarium solani infection in tropical countries. In this study, we examined the proteome profile of tear samples from A. flavus keratitis patients at various stages of infection. The proteome was profiled using 2D PAGE and the protein levels were quantified using 2D DIGE. Alpha-1-antitrypsin, apolipoprotein, haptoglobin, lactoferrin and albumin were up regulated while cystatin SA III precursor, lacrimal lipocalin precursor, lacritin precursor and Zinc alpha-2 glycoprotein (ZAG) were down regulated in tear fluid. In the case of ZAG all proteoforms were down regulated as the disease progressed from early to late stage of infection. Western blot analysis confirmed the results observed using DIGE. Further, there were no gender specific differences in the levels of ZAG expression in keratitis patient tear film. Published results show up regulation of ZAG in Fusarium keratitis patient tear indicating subtle changes in the early events of host response to these two fungal pathogens. We conclude that ZAG level could be used as an indicator of A. flavus or F. solani infection, even during the early stage of the disease.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Parthiban N,Sampath NL,JeyaMaheshwari J,Prajna NV,Lalitha P,Dharmalingam Kdoi
10.1016/j.exer.2019.107700subject
Has Abstractpub_date
2019-09-01 00:00:00pages
107700eissn
0014-4835issn
1096-0007pii
S0014-4835(19)30178-2journal_volume
186pub_type
杂志文章abstract::To investigate the nature of the 140 kDa glycoprotein in the trabecular meshwork, polypeptides were extracted with either urea/sodium dodecyl sulfate (SDS)/beta-2-mercaptoethanol (BME) or guanidine hydrochloride followed by pepsin digestion. After electrophoresis and immunoblotting with anti-type-VI-collagen antibodie...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1006
更新日期:1994-02-01 00:00:00
abstract::The purpose of this study was to determine whether endothelin B (ETB) receptor levels in the optic nerve are related to retinal ganglion cell (RGC) loss in a model of chronic endothelin-1 (ET-1) induced optic neuropathy. RGCs of adult Brown Norway rats were first retrogradely labeled with fluorochrome from the superio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.09.009
更新日期:2009-03-01 00:00:00
abstract::The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90011-8
更新日期:1983-02-01 00:00:00
abstract::The purpose of this study was to investigate the impact of different diffusion times of mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. Scleral quadrants of eight human donor eyes were exposed to sponges soaked with MMC for an application time of 1 min. After irrig...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0901
更新日期:2000-11-01 00:00:00
abstract::The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90093-6
更新日期:1989-08-01 00:00:00
abstract::Arf encodes an important tumor suppressor, p19(Arf), which also plays a critical role to control hyperplasia in the primary vitreous during mouse eye development. In the absence of Arf, mice are born blind and display a phenotype closely mimicking severe forms of the human eye disease, persistent hyperplastic primary ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.01.004
更新日期:2016-04-01 00:00:00
abstract::γ-secretase inhibitor has been shown to promote intestinal goblet cell differentiation. We now demonstrated that the in vitro addition of γ-secretase inhibitor in the culture of human conjunctival epithelial cells significantly promoted the differentiation of conjunctival goblet cells with typical droplet-like phenoty...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.04.001
更新日期:2014-06-01 00:00:00
abstract::The retinal pigment epithelium is a transporting epithelium that helps regulate the volume and composition of the subretinal space surrounding photoreceptor outer segments. The capacity of the RPE to actively transport Na+ and K+ between the retina and the blood supply depends on the localization of the Na+, K(+)-ATPa...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1144
更新日期:1994-11-01 00:00:00
abstract::The proteasomal pathway is responsible for processes essential for cell viability, including the selective degradation of oxidized proteins. An age-dependent loss in proteasome function has been reported in many tissues, but has not been examined in the retina. In this study, we evaluated proteasome function and prote...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::The interaction of keratocytes with extracellular matrix components plays an important role in the maintenance of corneal transparency and shape as well as in the healing of corneal wounds. In particular, the interaction of these cells with collagen and cell-mediated collagen contraction contribute to wound closure. E...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107933
更新日期:2020-02-01 00:00:00
abstract::Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants f...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.006
更新日期:2006-11-01 00:00:00
abstract::Retinal pigment epithelial (RPE) cells possess the potential to transdifferentiate into myofibroblasts after stimulation with transforming growth factor beta (TGFbeta) and are implicated in the pathogenesis of proliferative vitreoretinopathy. In this study we evaluated how TGFbeta2 and various extracellular matrix (EC...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.12.007
更新日期:2006-07-01 00:00:00
abstract::Glaucoma is a leading cause of irreversible blindness worldwide. Lowering intraocular pressure (IOP) is the only strategy documented to delay the appearance and retard the progression of vision loss. One major approach for lowering IOP is to slow the rate of aqueous humor formation by the ciliary epithelium. As discus...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2008.11.005
更新日期:2009-04-01 00:00:00
abstract::Photoreceptor degeneration in human photoreceptor dystrophies and in the relevant animal models has been thought to be executed by one common mechanism -- caspase-mediated apoptosis. However, recent experiments have challenged this concept. In previous experiments, analyzing gene expression in the degenerating rd/rd m...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.01.014
更新日期:2006-08-01 00:00:00
abstract::The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
更新日期:1996-04-01 00:00:00
abstract::The purpose of this investigation was to explore the combined effects of single nucleotide polymorphisms (SNPs) within LFA-1/ICAM-1/GSK-3β pathway and environmental hazards on susceptibility to Graves' opthalmopathy (GO) among a Chinese Han population. Altogether 305 GO patients and 283 Graves' disease (GD) subjects w...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.05.022
更新日期:2018-09-01 00:00:00
abstract::The transcription factor Prox1 acts in rodent retinogenesis, at least in promoting cell cycle withdrawal and horizontal cell production. In the mature retina, this protein is detected at the inner nuclear layer of all vertebrate groups. We have made a neurochemical characterisation of Prox1(+) cell types in two differ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.10.015
更新日期:2010-02-01 00:00:00
abstract::Retinal pigment epithelial cells (RPEs) are thought to be one of the main components of fibrous membrane observed in eyes with proliferative vitreo-retinopathy. We investigated the signalling mechanisms of TGF-beta2-dependent collagen gel contraction by RPEs. An in vitro type I collagen gel contraction assay was perfo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.09.024
更新日期:2006-05-01 00:00:00
abstract::Porcine models of ophthalmological diseases are often used in pre-clinical translational studies due to pigs' similarities to humans. In particular, the iodoacetic acid (IAA) model of photoreceptor degeneration seems to mimic well the endstage phenotype of human pathologies as retinitis pigmentosa and age-related macu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107979
更新日期:2020-04-01 00:00:00
abstract::A specialized microenvironment or niche, which regulates maintenance, self-renewal, activation, and proliferation of stem cells by external signals, is one of the key prerequisites for stem cell function. However, the parameters determining the limbal stem cell niche are not yet defined. In order to characterize the r...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.08.020
更新日期:2007-12-01 00:00:00
abstract::The purpose of this study was to investigate the effects of abnormal-cannabidiol (abn-cbd), a non-psychoactive cannabinoid agonist, on aqueous humor outflow via the trabecular meshwork (TM) of porcine eye, and to examine the involvement of a non-CB1/CB2 cannabinoid receptor and the p42/44 mitogen-activated protein kin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.05.001
更新日期:2012-07-01 00:00:00
abstract::Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90004-e
更新日期:1990-01-01 00:00:00
abstract::Various concentrations of three dopamine antagonists (fluphenazine, haloperidol and sulpiride) with different affinities to the receptor subtypes were applied in order to test their effect on responses from outer (isolated PIII-component of the ERG), middle (b-wave) and inner (optic nerve response, ONR) retinal layers...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90038-g
更新日期:1991-04-01 00:00:00
abstract::The purpose of this study was to examine the effects of myopia-inducing and myopia recovery conditions on the scleral biomechanics of enucleated eyes of young chicks. Enucleated eyes from 5-day old chicks, with fiducial markers attached at 5 locations on the external sclera, were placed in a custom-built chamber fille...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.07.004
更新日期:2014-10-01 00:00:00
abstract::Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.11.004
更新日期:2012-01-01 00:00:00
abstract::Conjunctival impression cytology samples from patients with meibomian gland dysfunction (MGD), dry eye (DE), and healthy subjects (CT) were collected for determination of the degree of squamous metaplasia (SM) by PAS-hematoxylin staining and for comparative proteomic analyses by 2D-DIGE. The protein spots with discrim...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.03.006
更新日期:2018-02-01 00:00:00
abstract::The aim of this study was to develop consistently focal elevated choroidal masses of human choroidal melanoma in immunosuppressed rabbits and to correlate the visualization of prognostically significant microcirculation patterns from confocal indocyanine green angiography with histologic microcirculation patterns. A h...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0650
更新日期:1999-06-01 00:00:00
abstract::The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0952
更新日期:2001-03-01 00:00:00
abstract::The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(95)80001-8
更新日期:1995-02-01 00:00:00
abstract::This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.07.007
更新日期:2013-10-01 00:00:00