Abstract:
:A specialized microenvironment or niche, which regulates maintenance, self-renewal, activation, and proliferation of stem cells by external signals, is one of the key prerequisites for stem cell function. However, the parameters determining the limbal stem cell niche are not yet defined. In order to characterize the role of basement membrane (BM) and extracellular matrix components in the generation of a microenvironmental niche for limbal stem and progenitor cells, we extensively analyzed the topographical variations of the BM zone of human ocular surface epithelia using immunohistochemistry and a large panel of antibodies to most of the presently described intrinsic and associated BM components. Apart from BM components uniformly expressed throughout all ocular surface epithelia (e.g. type IV collagen alpha5 and alpha6 chains, collagen types VII, XV, XVII, and XVIII, laminin-111, laminin-332, laminin chains alpha3, beta3,and gamma2, fibronectin, matrilin-2 and -4, and perlecan), the BM of the limbal epithelium shared many similarities with that of the conjunctival epithelium, including positive labelling for type IV collagen alpha1 and alpha2 chains, laminin alpha5, beta2, and gamma1 chains, nidogen-1 and -2, and thrombospondin-4, whereas type IV collagen alpha3, type V collagen, fibrillin-1 and -2, thrombospondin-1, and endostatin were present in the corneal BM, but lacking or more weakly expressed in the limbal and conjunctival BMs. As compared to both the corneal and conjunctival BMs, the limbal BM showed a markedly increased immunoreactivity for laminin alpha1, alpha2, beta1 chains, and agrin, and a specific but patchy immunoreactivity for laminin gamma3 chain, BM40/SPARC, and tenascin-C, which co-localized with ABCG2/p63/K19-positive and K3/Cx43/desmoglein/integrin-alpha2-negative cell clusters comprising putative stem and early progenitor cells in the basal epithelium of the limbal palisades. Components that were particularly expressed in the corneal-limbal transition zone included type XVI collagen, fibulin-2, tenascin-C/R, vitronectin, bamacan, chondroitin sulfate, and versican, all of which co-localized with vimentin-positive cell clusters comprising putative late progenitor cells in the basal epithelium. This pronounced heterogeneity of the BM in the limbal area, both in the region of limbal palisades and the corneal-limbal transition zone, appears to be involved in providing unique microenvironments for corneal epithelial stem and late progenitor cells. Identification of specific niche parameters might not only help to understand limbal stem cell regulation, but also to improve their selective enrichment and in vitro expansion for therapeutic strategies.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Schlötzer-Schrehardt U,Dietrich T,Saito K,Sorokin L,Sasaki T,Paulsson M,Kruse FEdoi
10.1016/j.exer.2007.08.020subject
Has Abstractpub_date
2007-12-01 00:00:00pages
845-60issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(07)00246-1journal_volume
85pub_type
杂志文章abstract::The aim of the study was to investigate the molecular genetics of uveal melanoma (UM) metastases and correlate it with disease progression. Twelve pathologically confirmed UM metastases from 11 patients were included. Molecular genetic alterations in chromosomes 3 (including the BAP1 region), 8q, 6p, and 1p were inves...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2012.04.010
更新日期:2012-07-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2002-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90071-4
更新日期:1983-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2004-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90183-0
更新日期:1984-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1006
更新日期:1994-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90138-1
更新日期:1985-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90023-n
更新日期:1990-09-01 00:00:00
abstract:PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1992-03-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
abstract::Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1151
更新日期:1994-12-01 00:00:00
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journal_title:Experimental eye research
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pub_type: 杂志文章
doi:10.1006/exer.1998.0517
更新日期:1998-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90034-r
更新日期:1990-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0062
更新日期:1996-05-01 00:00:00