Abstract:
:To facilitate the peeling of internal limiting membrane or epiretinal membrane in vitreoretinal surgery, trypan blue (TB) and indocyanine green (ICG) have been used. However, the cytotoxicity of these dyes have been concerned. The aim of this study was to investigate the neurotoxic effects of TB on rat retinal ganglion cells (RGCs) and compare the effect of TB with ICG. Rat RGCs were purified by a two-step immunopanning procedure. In short-time exposure experiments, purified RGCs cultured for 3 days were exposed to 600 mgL(-1) TB, 1500 mgL(-1) TB and 1500 mgL(-1) ICG for 10 sec to 30 min. The number of viable RGCs was counted after 12 hr in culture. In long-time exposure experiments, purified RGCs were cultured for 3 days in TB solutions ranging from 2 to 800 mgL(-1) or in ICG solutions at concentrations from 2 to 250 mgL(-1). Then the number of viable cells was counted. Exposure to 600 mgL(-1) TB, 1500 mgL(-1) TB and 1500 mgL(-1) ICG for short time from 10 sec to 30 min caused a time-dependent damage to RGCs. There was no significant difference in cytotoxicity to RGCs between TB and ICG in short-time exposure. In long-time exposure, TB as well as ICG showed neurotoxic effect on RGCs in a dose-dependent manner. Fifty percentage inhibitory concentration (IC50) of TB to RGCs was calculated as 115 mgL(-1), while that of ICG was 33 mgL(-1). In conclusion, TB induced neurotoxic effect on RGCs in a dose- and time-dependent manner. During the short-time exposure in surgery, there is likely no difference in neurotoxic effect on RGCs between TB and ICG. However, once the dyes are left in the eyes after surgery, TB may show less toxicity on RGCs than ICG.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Jin Y,Uchida S,Yanagi Y,Aihara M,Araie Mdoi
10.1016/j.exer.2005.02.010keywords:
subject
Has Abstractpub_date
2005-10-01 00:00:00pages
395-400issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(05)00062-Xjournal_volume
81pub_type
杂志文章abstract::Thioltransferase (TTase) activity was identified and partially purified from the ocular tissue for the first time. The enzyme activity depended on the presence of reduced glutathione (GSH), glutathione reductase (GR) and NADPH to reduce the disulfide bond in a synthetic substrate, hydroxyl ethyl disulfide (HEDS). Maxi...
journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1193
更新日期:1994-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0234
更新日期:1997-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90044-u
更新日期:1990-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90093-6
更新日期:1989-08-01 00:00:00
abstract::Iron is an essential biometal in the aqueous humor, the principal source of nutrients for the avascular cornea and the lens. Here, we explored whether the ciliary body (CB), the source of aqueous humor, transports iron, and if the prion protein (PrPC) facilitates this process as in the outer retina. Using a combinatio...
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doi:10.1016/j.exer.2018.05.031
更新日期:2018-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0512
更新日期:1998-08-01 00:00:00
abstract::Laser Doppler flowmetry (LDF) measures the flux of red blood cells in a vascular network embedded in tissue. In the present report, choroidal and ciliary body blood flow was measured continuously using an LDF technique in pentobarbital anesthetized cats. Blood flow measurements were made from the choroid beneath the l...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90068-p
更新日期:1991-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.02.011
更新日期:2006-10-01 00:00:00
abstract::A single intraperitoneal dose of endotoxin (lipopolysaccharide or LPS) induces an acute inflammatory response in the uveal tract of rats. This inflammation is characterized by a breakdown of the blood/aqueous barrier within 3 hr after the LPS and the subsequent development of clinical disease and a cellular infiltrate...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90065-4
更新日期:1984-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80047-7
更新日期:1987-06-01 00:00:00
abstract::The reversible phosphorylation of proteins plays essential roles in regulating various cellular events, and is regulated by the opposing actions of protein kinases and protein phosphatases. Protein kinases in the lens system have been well studied, but very little is known about lens protein phosphatases. Protein phos...
journal_title:Experimental eye research
pub_type: 更正并重新发布的文章,杂志文章
doi:
更新日期:2004-12-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1006/exer.1997.0422
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更新日期:2011-12-01 00:00:00
abstract::The Otx2 gene encodes a transcription factor essential for the normal development of brain, cerebellum, pineal gland, and eye. In the retina, Otx2 has essential functions from early embryogenesis to adulthood. As soon as the optic vesicle is formed, the gene is required for retinal pigment epithelium specification. Ot...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.03.007
更新日期:2013-06-01 00:00:00
abstract::Retinal degeneration (RD) results from photoreceptor apoptosis. Cell transplantation, one potential therapeutic approach, requires expandable stem cells that can form mature photoreceptors when differentiated. Freshly dissociated primary retinal cells from postnatal day 2-6 (PN2-6) mouse retina can give rise, post-tra...
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更新日期:2010-10-01 00:00:00
abstract::Lack of an optimal in vitro model of human corneal epithelial (HCE) cells is a major limitation in studying normal functions and gene regulations in HCE. Moreover, availability of a multi-layered HCE culture can reduce the usage of animals in the toxicity testing of consumer products. We have developed tetracycline-re...
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更新日期:2003-10-01 00:00:00
abstract::For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...
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更新日期:2018-07-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2009.06.020
更新日期:2009-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00
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journal_title:Experimental eye research
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更新日期:2007-05-01 00:00:00
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doi:10.1016/j.exer.2011.07.012
更新日期:2011-07-29 00:00:00