Abstract：
:Laser Doppler flowmetry (LDF) measures the flux of red blood cells in a vascular network embedded in tissue. In the present report, choroidal and ciliary body blood flow was measured continuously using an LDF technique in pentobarbital anesthetized cats. Blood flow measurements were made from the choroid beneath the lateral and superior rectus muscles and from the surface above the ciliary body 2-3 mm posterior to the limbus. In some experiments, the sympathetic nerve to the eye was stimulated; in others, intraocular pressure (IOP) was monitored manometrically with a controlled saline infusion delivered into the anterior chamber to gradually elevate IOP. Norepinephrine (NE) and methacholine (MC) also were tested for their effects on choroidal blood flow when given intravenously and intra-arterially. Transient bilateral carotid artery occlusions produced a significant drop in blood flow, but flow did not decrease to zero probably due to collateral contribution of the vertebral circulation. Sympathetic nerve stimulation produced a frequency-dependent decrease in both choroidal and ciliary body blood flow with the latter exhibiting a lower threshold and a greater magnitude of effect. Gradual elevation of IOP produced a proportional decrease in choroidal blood flow. The level of intraocular pressure required to decrease blood flow was dependent on the existing ocular perfusion pressure. Both intravenous and intra-arterially administered NE produced dose-dependent decreases of choroidal blood flow despite graded increases of systemic arterial blood pressure. In contrast, MC produced dose-dependent increases of choroidal blood flow in conjunction with graded decreases of systemic blood pressure. The results of the present report demonstrate the validity of the LDF technique for performing continuous reading of blood flow changes in the choroid and ciliary body of experimental animals.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Gherezghiher T,Okubo H,Koss MCdoi
10.1016/0014-4835(91)90068-psubject
Has Abstractpub_date
1991-08-01 00:00:00pages
151-6issue
2eissn
0014-4835issn
1096-0007pii
0014-4835(91)90068-Pjournal_volume
53pub_type
杂志文章abstract：:Proliferative diabetic retinopathy (PDR) is characterized by intraocular formation of fibroglial vascularized tissue by active vasoproliferative mechanisms. Using immunocytochemistry, we have studied changes in the distribution pattern of fibronectin (FN), laminin (LN), vitronectin (VN) and their receptors in the newl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80079-x
更新日期：1995-01-01 00:00:00
abstract：:Exposure to hyposmotic solution causes release of ATP from lens cells via hemichannels. Because hemichannel opening feasibly could swamp the cells with calcium, we carried out studies to measure the magnitude of the increase in cytoplasmic calcium concentration caused by hemichannel opening. In studies on porcine lens...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.012
更新日期：2015-03-01 00:00:00
abstract：:Herpes simplex virus type-1 (HSV-1) infection leads to impaired corneal sensation and, in severe cases, to corneal ulceration, melting and perforation. Here, we explore the potential therapeutic action of pigment epithelial-derived factor (PEDF) plus docosahexaenoic acid (DHA) on corneal inflammation and nerve regener...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.06.015
更新日期：2017-08-01 00:00:00
abstract：:The histopathological features of uveitis and retinal vasculitis in acute experimental allergic encephalomyelitis (EAE) were investigated using light and electron microscopy. Lewis rats were immunized by spinal cord homogenate, complete Freund's adjuvant and Bordetella pertussis. The eyes of rats with EAE exhibited va...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1024
更新日期：1993-02-01 00:00:00
abstract：:Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90183-0
更新日期：1984-06-01 00:00:00
abstract：:The lysosomal proteases cathepsin B and dipeptidyl peptidase II (DPP II) were identified biochemically in the supernatant fraction of rat and guinea-pig lens homogenates, using as substrates CBZ-Arg-Arg-4-methylcoumarin-7-amide (AMC) (5 microM, pH 6.0, 37 degrees C for cathepsin B, and Lys-Ala-AMC or Lys-Pro-AMC (5 mi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80038-6
更新日期：1987-06-01 00:00:00
abstract：:Endostatin is an endogenous angiogenesis inhibitor which requires E-selectin for its antiangiogenic activity. The aim of this study was to investigate the expression of endostatin in human choroidal neovascular membranes (CNV) secondary to age-related macular degeneration (AMD) with regard to vascularization and proli...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.12.017
更新日期：2006-08-01 00:00:00
abstract：:Epithelial cells in explants from rat lenses differentiate into fibre cells when cultured with retina conditioned medium (RCM). This differentiation is characterised by cell elongation, the accumulation of alpha-crystallin and the onset of synthesis of the fibre specific beta- and gamma-crystallins. To determine the e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80083-6
更新日期：1988-02-01 00:00:00
abstract：:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0765
更新日期：2000-02-01 00:00:00
abstract：:In a variety of tissues including gastrointestinal mucosa, rebamipide (REB) provides cytoprotection, prevents inflammation, and promotes wound healing. Clinically, REB ophthalmic dispersions are used to treat diabetic keratopathy. In this study, we investigated the optimal particle size of REB to promote corneal wound...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.07.003
更新日期：2016-10-01 00:00:00
abstract：:We have investigated: (a) Phospholipid composition and phosphoinositide-PO4 turnover in rabbit cornea tissues; and (b) the effects of adrenergic and serotonergic agonists on breakdown of phosphoinositides in the rabbit cornea. The data obtained from these studies can be summarized as follows: (1) in the cornea phospha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80047-7
更新日期：1987-06-01 00:00:00
abstract：:Reactive nitrogen species, in particular, peroxynitrite (ONOO(-)) have been proposed to play an important role in the pathogenesis of endotoxin-induced uveitis (EIU). Tyrosine nitration by ONOO(-) has been shown in other model systems to inhibit the activity of the superoxide anion quenching enyzme, manganese superoxi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.1141
更新日期：2002-04-01 00:00:00
abstract：:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1156
更新日期：1993-11-01 00:00:00
abstract：:Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2004.06.011
更新日期：2004-09-01 00:00:00
abstract：:Microaneurysms, acellular capillaries and pericyte ghosts are characteristic of diabetic retinopathy, but it is not clear what causes these lesions or whether they are causally related to each other. The distribution of microaneurysms, acellular capillaries and pericyte ghosts has been evaluated in two animal models o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80069-7
更新日期：1995-05-01 00:00:00
abstract：:An extraneous exon-skipping mRNA encodes an altered form of a light-absorbing opsin in human retina and pigment epithelium (RPE). The predicted protein variant differs from full-length RPE-retinal G protein-coupled receptor (RGR) by having an in-frame deletion of exon 6, which contains the entire sixth transmembrane d...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.11.013
更新日期：2006-07-01 00:00:00
abstract：:The effect of angiotensin II was studied in vitro on ring segments of bovine retinal resistance arteries (i.d. 126-271 microns) and posterior ciliary arteries (i.d. 207-1153 microns). Although the retinal resistance arteries were responsive to 5-hydroxytryptamine, prostaglandin F2 alpha, and changes in extracellular K...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90034-r
更新日期：1990-05-01 00:00:00
abstract：:Myofibroblast development and haze generation in the corneal stroma is mediated by cytokines, including transforming growth factor-beta (TGF-beta), and possibly other cytokines. This study examined the effects of stromal PDGF-beta blockade on the development of myofibroblasts in response to -9.0 diopter photorefractiv...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.12.006
更新日期：2009-05-01 00:00:00
abstract：:Age-related macular degeneration (AMD) is the major cause of blindness for people over 60. In the "wet" form of AMD compounds targeting growth factor signaling pathways such as VEGF have been a major focus for therapeutic interventions. In a previously developed rat model of CNV, we utilized two receptor tyrosine kina...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.01.022
更新日期：2007-05-01 00:00:00
abstract：:The regulation of acidic amino-acid transport across the retinal pigment epithelium is of particular interest since glutamate and possibly aspartate have been identified as putative neurotransmitters in the retina, at the level of the photoreceptor cell. The present study, designed to measure the rate of acidic amino-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80088-4
更新日期：1986-08-01 00:00:00
abstract：:Retinal stimulation with high spatial resolution requires close proximity of electrodes to target cells. This study examines the effects of material coatings and 3-dimensional geometries of subretinal prostheses on their integration with the retina. A trans-scleral implantation technique was developed to place microfa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.09.018
更新日期：2009-01-01 00:00:00
abstract：:The elimination of spermine, the end product of cellular polyamine, from the retina to the blood across the blood-retinal barrier (BRB) was investigated. The in vivo microdialysis study revealed that the elimination of [(3)H]spermine from vitreous humor after vitreous bolus injection was in a biexponential manner. The...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:Exfoliation syndrome (XFS) is an important risk factor for glaucoma (XFG) worldwide. LOXL1 variants are highly associated with XFS in most populations; however, the high frequency of risk alleles in normal individuals and the reversal of risk alleles in different ethnic populations suggest that other factors contribut...
journal_title：Experimental eye research
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.exer.2015.08.004
更新日期：2015-10-01 00:00:00
abstract：:Purpose of the study was to develop and assess a novel controlled drug delivery system of latanoprost acid (LA). Poly(lactide)/Monomethoxy-poly(ethyleneglycol) (PLA-PEG) nanoparticles (NPs) were prepared using an emulsification-solvent evaporation technique. NPs were characterized in vitro according to their size, ζ-p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.04.007
更新日期：2013-07-01 00:00:00
abstract：:There is currently no available method to efficiently deliver proteins across the plasma membrane of photoreceptor or retinal pigment epithelium (RPE) cells in vivo. Thus, current clinical application of recombinant proteins in ophthalmology is limited to the use of proteins that perform their biological function extr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.009
更新日期：2016-04-01 00:00:00
abstract：:The present study determined whether topical latanoprost, a prostaglandin (PG) F(2alpha) analog, influences the induction of anterior chamber-associated immune deviation (ACAID), corneal neovascularization (NV) or survival of corneal allografts in mice. BALB/c mice received topical latanoprost or PGE(2) once or multip...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.012
更新日期：2008-02-01 00:00:00
abstract：:Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.019
更新日期：2017-01-01 00:00:00
abstract：:Deficient cGMP-phosphodiesterase (cGMP-PDE) activity results in elevated levels of cGMP in the rd retina before any pathological signs are observed. Since the enzyme is present in rd retina, although it is barely activated by light, we determined whether its synthesis starts at the same time as that of cGMP-PDE in nor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80026-5
更新日期：1988-03-01 00:00:00
abstract：:Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.08.018
更新日期：2007-12-01 00:00:00
abstract：:The retinal pigment epithelium is a transporting epithelium that helps regulate the volume and composition of the subretinal space surrounding photoreceptor outer segments. The capacity of the RPE to actively transport Na+ and K+ between the retina and the blood supply depends on the localization of the Na+, K(+)-ATPa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1144
更新日期：1994-11-01 00:00:00
