Abstract:
:Purpose of the study was to develop and assess a novel controlled drug delivery system of latanoprost acid (LA). Poly(lactide)/Monomethoxy-poly(ethyleneglycol) (PLA-PEG) nanoparticles (NPs) were prepared using an emulsification-solvent evaporation technique. NPs were characterized in vitro according to their size, ζ-potential, drug entrapment efficiency and LA release. LA-loaded NPs (equivalent to 8.5 μg LA) were administered into the subconjunctival space of normotensive rabbits (group A). A free LA solution of the same drug content was subconjunctivally injected in a second rabbit group (group B), while blank NPs were administered in a third group (group C). A group of untreated animals (group D) served as the control. Intraocular pressure (IOP) was monitored for 8 consecutive days, using the Tono-pen XL. Aqueous humor (AH) levels of LA were evaluated for 6 days post-administration, by means of HPLC. Mean nanoparticle size was 80 nm. The drug entrapment efficiency was 18.3%. NPs sustained the release of LA over several days in vitro. Non-significant differences in baseline IOP were found between groups (p = 0.22). LA-loaded NPs exerted a significant hypotensive effect on group A, while IOP values remained significantly lower compared to the rest groups, throughout the study (p = 0.04). LA AH concentrations in group B continuously decreased with time, while LA levels in group A steadily increased. On day 6, LA levels were higher in group A compared to group B (344 ± 73.5 ng/ml and 228 ± 41.01 ng/ml, respectively). No adverse effects were observed. In conclusion, after subconjunctival administration, the LA-loaded NPs provided sustained LA delivery in vivo. They appear to be a promising system for the controlled subconjunctival delivery of LA.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Giarmoukakis A,Labiris G,Sideroudi H,Tsimali Z,Koutsospyrou N,Avgoustakis K,Kozobolis Vdoi
10.1016/j.exer.2013.04.007subject
Has Abstractpub_date
2013-07-01 00:00:00pages
29-36eissn
0014-4835issn
1096-0007pii
S0014-4835(13)00095-Xjournal_volume
112pub_type
杂志文章abstract::Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss, while its pathogenesis is still poorly understood. Focal adhesion kinase (FAK), a non-receptor tyrosine kinase, plays a crucial role in linking signals initiated by both the extracellular matrix (ECM) and solub...
journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90257-f
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.exer.2012.02.009
更新日期:2012-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.exer.2017.12.009
更新日期:2018-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90023-n
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1186
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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更新日期:2017-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2015-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1083
更新日期:1994-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.02.010
更新日期:2010-05-01 00:00:00