Abstract：
:Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells for 1 week or human RPE for 3 weeks, they density arrest, assume a differentiated morphology, and produce pigment and mRNA for CRALBP (all differentiated features of RPE). Human RPE grown on a laminin substratum or grown in media supplemented with basic fibroblast growth factor (basic FGF), assume a differentiated morphology for up to 3 weeks, and this is maintained for several months when the cells are grown on laminin in the presence of basic FGF and heparin. With the latter conditions, the cells also produce mRNA for CRALBP. Human RPE grown on bovine corneal endothelial cell matrix treated with neutralizing antibodies to basic FGF or laminin or agents that displace FGF from extracellular matrix (suramin or protamine), do not assume a differentiated morphology and express less CRALBP mRNA than RPE grown on bovine corneal endothelial cell matrix treated with antibodies to type IV collagen. These data suggest that the extracellular matrix may facilitate RPE expression of differentiated features and that laminin and basic FGF may be important components.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Campochiaro PA,Hackett SFdoi
10.1006/exer.1993.1158subject
Has Abstractpub_date
1993-11-01 00:00:00pages
539-47issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71158-9journal_volume
57pub_type
杂志文章abstract：:Innervated myotendinous cylinders (IMCs) in rabbit extraocular muscles (EOMs) were identified for the first time. The nature of IMC nerve terminals was demonstrated by means of electron microscopy and double fluorescent staining. The distal EOM portions of four rabbits of different age and sex were prepared for transm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1085
更新日期：2001-12-01 00:00:00
abstract：:Retinal inflammation is an integral component of many retinal diseases including diabetic retinopathy (DR), age-related macular degeneration (AMD) and retinopathy of prematurity (ROP). Inflammation is commonly initiated and perpetuated by myeloid-derived immune cells. In the retina, microglial cells are resident macro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.05.010
更新日期：2019-08-01 00:00:00
abstract：:Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90004-e
更新日期：1990-01-01 00:00:00
abstract：:Hypertension is an independent risk factor for diabetic retinopathy, yet anti-hypertensive medications such as blockade of angiotensin II do not completely protect against vision-threatening vascular disease. We hypothesized that the potent vasoactive factor, endothelin (ET), is up-regulated in diabetic retinopathy an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.06.025
更新日期：2018-11-01 00:00:00
abstract：:The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.019
更新日期：2006-01-01 00:00:00
abstract：:Two previous studies have shown that N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of neuronal nitric oxide synthase, protects retinas of albino rats and mice from damaging levels of light. The aims of the present study were two-fold: (1) to confirm the protective effect of L-NAME on wild type albino rats ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00334-2
更新日期：2003-04-01 00:00:00
abstract：:A duplex fluorescence assay to assess the viability of cells cultured in multi-well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture or assay medium, or transfer of cells or cell supernatant. The method uses freshly prepared reagents and doe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.05.011
更新日期：2017-08-01 00:00:00
abstract：:The autonomic innervation in the anterior chamber (AC) structures might play an efferent role in neural intraocular pressure (IOP) regulation, the center of which is thought to be located in the hypothalamus. In this study, we identified the efferent pathway from the hypothalamus to the autonomic innervation in the AC...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108367
更新日期：2021-01-01 00:00:00
abstract：:Electrical field stimulation produced contractions of the rabbit isolated iris sphincter muscle which were completely blocked by tetrodotoxin (10(-6) M). Low frequency (0.16-5 Hz) responses were atropine-resistant, but were prevented by sensory (trigeminal) denervation, and were considered to be owing to antidromic st...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90099-x
更新日期：1984-02-01 00:00:00
abstract：:Photovoltaic arrays (PVA) implanted into the subretinal space of patients with retinitis pigmentosa (RP) are designed to electrically stimulate the remaining inner retinal circuitry in response to incident light, thereby recreating a visual signal when photoreceptor function declines or is lost. Preservation of inner ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.09.004
更新日期：2014-11-01 00:00:00
abstract：:The purpose of this study was to investigate the impact of different diffusion times of mitomycin-C (MMC) on the intrascleral concentration vs depth profile of MMC in an experimental model. Scleral quadrants of eight human donor eyes were exposed to sponges soaked with MMC for an application time of 1 min. After irrig...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0901
更新日期：2000-11-01 00:00:00
abstract：PURPOSE:This microscopic study was undertaken to compare the melanocytes of peripheral iridectomy specimens from two eyes that had latanoprost-induced iris darkening (LIID) with iridectomies taken from the fellow untreated eyes. METHODS:The two patients in this study were the ones who underwent LIID in the latanoprost...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.08.009
更新日期：2003-12-01 00:00:00
abstract：:Although the presence of 'cartilage-specific' collagens in the eye has been documented earlier, very little is known about their synthesis rates during ocular development, growth and aging. The purpose of the present study was to follow changes in the mRNA levels and distribution of key components of the extracellular...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0972
更新日期：2001-04-01 00:00:00
abstract：:Recent evidence suggests that mucosal epithelial cells are capable of actively participating in immune reactions via expression of surface antigens, such as adhesion molecules, and synthesis of cytokines. This appears to be important in the pathophysiology of non-ocular allergic disorders. The objectives of the experi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0528
更新日期：1998-11-01 00:00:00
abstract：:The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80042-3
更新日期：1988-06-01 00:00:00
abstract：:Utilizing cultured lenses from normal and homozygous glutathione peroxidase (GSHPx-1) knockout mice and inhibitors for GSSG Reductase (GSSG Red), 1,3-bis(2-chlorethyl)-1-nitrosourea (BCNU) and catalase (Cat), 3-aminotriazole (3-AT), the ability to degrade H2O2 was examined at two H2O2 concentrations, 300 microM and 80...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0250
更新日期：1997-03-01 00:00:00
abstract：:Lens epithelial cells undergo a dramatic transformation during the process of differentiation into elongated fiber cells. The membrane-associated actin cytoskeleton is likely to play a critical role in the stabilization and maintenance of the highly elongated fiber cell shape. Tropomodulin is a tropomyosin-binding pro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0111
更新日期：1996-08-01 00:00:00
abstract：:Neovascular eye diseases such as wet age-related macular degeneration and proliferative diabetic retinopathy are two of the most common causes of irreversible visual loss. Although mediated by vascular endothelial growth factor (VEGF), the mechanisms of these diseases are not fully understood. Molecular inhibitors of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.09.010
更新日期：2011-11-01 00:00:00
abstract：:Müller cells (MC) represent a key element for the metabolic and functional regulation of the vertebrate retina. The aim of the present study was to test the feasibility of a new method for the in-vivo detection and quantification of extrafoveal MC in human retina. We developed a new approach to isolate and analyse ext...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108183
更新日期：2020-10-01 00:00:00
abstract：:The similarities between glaucoma and mitochondrial optic neuropathies have driven a growing interest in exploring mitochondrial function in glaucoma. The specific loss of retinal ganglion cells is a common feature of mitochondrial diseases - not only the classic mitochondrial optic neuropathies of Leber's Hereditary ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.07.015
更新日期：2011-08-01 00:00:00
abstract：:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increase...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
abstract：:The purpose of these experiments was to develop a hypothesis to explain activation of m-calpain in cataractogenesis observed in rodents. The in vitro model used to study m-calpain activation was to correlate breakdown of the 'reporter' protein alpha-crystallin with the appearance of activated m-calpain using protein s...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0234
更新日期：1997-04-01 00:00:00
abstract：:Iron accumulation in the retina is associated with the development of age-related macular degeneration (AMD). IV iron is a common method to treat iron deficiency anemia in adults, and its retinal manifestations have not hitherto been identified. To assess whether IV iron formulations can be retina-toxic, we generated ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.08.008
更新日期：2016-10-01 00:00:00
abstract：:Hypoxia-inducible factor (HIF) signaling cascade plays a critical role in angiogenesis by activating the transcription of genes encoding angiogenic growth factors. This study evaluated the effects of YC-1, a HIF-1 inhibitor, on the morphological, biochemical and molecular changes in human retinal microvascular endothe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.06.013
更新日期：2009-11-01 00:00:00
abstract：:Early stages of glaucoma and optic neuropathies are thought to show inner retina remodeling and functional changes of retinal ganglion cells (RGCs) before they die. To assess RGC functional plasticity, we investigated the contrast-gain control properties of the pattern electroretinogram (PERG), a sensitive measure of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.11.008
更新日期：2016-04-01 00:00:00
abstract：:In a variety of tissues including gastrointestinal mucosa, rebamipide (REB) provides cytoprotection, prevents inflammation, and promotes wound healing. Clinically, REB ophthalmic dispersions are used to treat diabetic keratopathy. In this study, we investigated the optimal particle size of REB to promote corneal wound...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.07.003
更新日期：2016-10-01 00:00:00
abstract：:Retinal lesions in the posterior pole of laboratory mice occur due to native, developmental abnormalities or as a consequence of environmental or experimental conditions. In this study, we investigated the rate and extent of retinal lesions as a result of prolonged ocular exposure following general anesthesia. Followi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.05.012
更新日期：2019-08-01 00:00:00
abstract：:Potentiation of retinal degeneration by intense light exposure, and its amelioration by an antioxidant, were studied in a rat model of Smith-Lemli-Opitz syndrome (SLOS), in comparison with normal (control) Sprague-Dawley rats. The SLOS model is created by treating rats with AY9944, a selective inhibitor of cholesterol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.08.008
更新日期：2006-03-01 00:00:00
abstract：:Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.02.007
更新日期：2019-04-01 00:00:00
abstract：:Collagen was isolated from bovine cornea and tested for reactivity towards platelets and IgG-coated polystyrene latex particles. The corneal collagen caused a dose- and temperature-dependent platelet aggregation in all human platelet-rich plasmas studied. As little as 0 . 31 micrograms of purified corneal collagen cou...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90122-7
更新日期：1983-03-01 00:00:00
