Abstract：
:Lens epithelial cells undergo a dramatic transformation during the process of differentiation into elongated fiber cells. The membrane-associated actin cytoskeleton is likely to play a critical role in the stabilization and maintenance of the highly elongated fiber cell shape. Tropomodulin is a tropomyosin-binding protein associated with actin filaments in a variety of terminally differentiated cell types where stable actin filament organization is required for cell function. We now present results of studies to determine the temporal expression of tropomodulin in the developing lens. In situ hybridization experiments detected expression of tropomodulin mRNA in the developing mouse lens in elongating cells with a pattern similar to that of the fiber specific beta- and gamma-crystallins. Tropomodulin mRNA expression first appeared around 11.5 days post-coitum in elongating cells in the posterior part of the lens vesicle. At later stages the signal for tropomodulin was present in the elongating cells at the lens equator and in cortical fiber cells; signal was absent from the epithelium. To investigate the possible link between tropomodulin expression and fiber differentiation we used a well-established lens epithelial explant culture system in which fiber differentiation is induced by fibroblast growth factor (FGF). Tropomodulin expression was only observed in FGF-treated explants in conjunction with morphologic changes characteristic of lens fiber cell differentiation. The appearance of tropomodulin during the process of fiber cell differentiation suggests that tropomodulin may be important for stabilization and/or determination of actin filament length.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Sussman MA,McAvoy JW,Rudisill M,Swanson B,Lyons GE,Kedes L,Blanks Jdoi
10.1006/exer.1996.0111subject
Has Abstractpub_date
1996-08-01 00:00:00pages
223-32issue
2eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90111-6journal_volume
63pub_type
杂志文章abstract：:This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
abstract：:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90017-5
更新日期：1986-01-01 00:00:00
abstract：:The phagocytosis of photoreceptor outer segments (OS) by the retinal pigment epithelium (RPE) is a receptor mediated process. A key component of this process is the receptor tyrosine kinase, Mer. RPE cells from the RCS rat, which lacks a functional mer gene, and do not express Mer protein, are able to bind OS, but are...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00158-1
更新日期：2003-09-01 00:00:00
abstract：:The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0952
更新日期：2001-03-01 00:00:00
abstract：:Corneal endothelial wound healing following scrape injury in the rabbit and cat is characterized by cell spreading and maintenance of a normal endothelial phenotype consisting of apically-localized, circumferential microfilament bands and cell border-associated ZO-1, a tight junction protein and marker for endothelial...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0211
更新日期：1997-02-01 00:00:00
abstract：:Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were isolated from frog retinal rod outer segment (ROS) membranes and their major molecular species determined. Saturated fatty acids are predominantly located on position-1 and polyunsaturates on position-2, although this assignment is not absolute. One-fifth...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90075-1
更新日期：1983-08-01 00:00:00
abstract：:By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80009-0
更新日期：1995-06-01 00:00:00
abstract：:Diabetic retinopathy is a complication of diabetes and a leading cause of vision loss among working-age adults. To assess whether the Wistar rat with Streptozotocin (STZ)-induced diabetes is a suitable animal model of human proliferative diabetic retinopathy we evaluated the vascular changes to assess the diabetic ret...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.025
更新日期：2019-07-01 00:00:00
abstract：:Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90048-1
更新日期：1989-09-01 00:00:00
abstract：:Preretinal pH was measured in the avascular region of 19 rabbit retinas during alternating cycles of light and dark, using a miniature, needle-type pH electrode. In 14 experiments with the rabbits breathing room air, cyclic changes in pH, decreasing in the dark and increasing in the light, were observed. The transitio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90106-o
更新日期：1991-12-01 00:00:00
abstract：:The objective of this study were to establish a cell line derived from porcine hyalocytes and to investigate the regulation of hyaluronan (HA) synthesis in response to cytokines. After 50 passages of the cells derived from porcine vitreous tissue, a cell line was generated. The immortalized cells showed fibroblastic m...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.006
更新日期：2007-10-01 00:00:00
abstract：:Mutations in the optic atrophy type 1 (OPA1) gene give rise to human autosomal dominant optic atrophy. The purpose of this study is to investigate OPA1 protein expression in the human retina and optic nerve. A rabbit polyclonal antiserum was generated using a fusion protein covering amino acids 647 to 808 of the human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.06.004
更新日期：2006-11-01 00:00:00
abstract：:Lactoferrin plays an important role in the defense against infections, including herpes simplex virus (HSV) keratitis. We studied the impact of three single nucleotide polymorphisms in the human lactoferrin gene on the susceptibility to HSV infections of the eye and the severity of such infections. Lactoferrin gene po...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.09.013
更新日期：2008-01-01 00:00:00
abstract：:Biochemical alterations in the composition of retinal capillary basement membrane components were investigated in galactosemic rats, an animal model that develops basement membrane lesions comparable to those of diabetic retinopathy. Normotensive Wistar-Kyoto rats fed a 30% galactose diet for 9 months developed signif...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90211-c
更新日期：1990-03-01 00:00:00
abstract：:Proliferative vitreoretinopathy (PVR) is a potentially blinding fibrotic complication. It is caused by the formation and contraction of epiretinal membranes (ERMs) that ultimately lead to retinal folds and traction retinal detachments. While multiple cell types have been identified in ERMs, retinal pigment epithelial ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.02.008
更新日期：2016-01-01 00:00:00
abstract：:A technology has recently been developed that allows for the rapid transduction of full-length functionally active proteins into intact tissue through intravenous injection and into cultured cells. This technology involves the fusion of an 11 amino acid sequence of the HIV TAT protein to the protein of interest. In th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.12.010
更新日期：2004-05-01 00:00:00
abstract：:Incorporated in the luminal glycocalyx of vascular endothelia (EC) are negatively charged microdomains (anionic sites). These sites are considered functionally important (a) in their interaction with circulating blood constituents, and (b) as a determinant of vascular permeability. The molecular composition of these E...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80134-4
更新日期：1995-10-01 00:00:00
abstract：:Traumatic optic neuropathy (TON) can occur following blunt trauma to the orbit and can lead to permanent vision loss. In this study, we investigated the effectiveness of elamipretide (MTP-131), a small mitochondrially-targeted tetrapeptide, in conjunction with etanercept, a tumor necrosis factor (TNF) inhibitor, as ne...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.108178
更新日期：2020-10-01 00:00:00
abstract：:Previous studies have shown that the inhibition of Rho kinase is involved in the regulation of outflow facility in the live rabbit eye and the enucleated porcine eye. However, it is unknown whether the Rho kinase inhibition will do the same in non-human primates. To determine if the Rho kinase inhibitor Y-27632 will r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.09.002
更新日期：2005-02-01 00:00:00
abstract：:We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolong...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.11.008
更新日期：2007-03-01 00:00:00
abstract：:The purpose of our study was to establish a system for culturing normal human conjunctival epithelial (NHCE) cells under serum-free culture conditions without compromising their ability to differentiate into a mucous epithelium. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing cat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.04.005
更新日期：2007-08-01 00:00:00
abstract：:Previous studies have shown that retinal damage induced by damaging light exposure is more severe in superior retina than inferior retina when measured along the vertical meridian of eyes. However, the extent of retinal damage over all retinal regions is not clear. Albino rats were exposed to bright light (5000 lux fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.06.002
更新日期：2008-09-01 00:00:00
abstract：:Identification of causal genetic defects for human diseases took a significant leap when the first generation DNA sequencing technologies enabled biologists extract sequence-based genetic information from living beings. However, these sequencing methods had unavoidable constraints of throughput, scalability, rapidity,...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.08.013
更新日期：2017-11-01 00:00:00
abstract：:It has been reported that chymase activity was increased in allergic conjunctivitis patients and this activity was correlated with the severity of the disease. However, the precise roles of chymase in allergic conjunctivitis are unclear, and whether chymase inhibitors are effective for allergic conjunctivitis has not ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.05.015
更新日期：2013-08-01 00:00:00
abstract：:This is a brief overview of multifaceted anatomical, experimental and clinical studies conducted by the author since 1955 on the optic nerve head circulation in health and disease. Conclusions, based on the accumulated information provided by these studies, are summarized. The studies on the pattern of blood supply of...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(05)80121-6
更新日期：1995-09-01 00:00:00
abstract：:The benzopyran BP (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) is a free radical scavenger that is structurally similar to alpha-tocopherol and has provided neuro-protection in a number of disease models where oxidative stress is a causative factor. A novel derivative of BP with improved lipid solub...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.04.007
更新日期：2011-07-01 00:00:00
abstract：:Evidence is presented that alpha-crystallin isolated at 37 degrees C exists as a species, alpha m, which has a minimum molecular weight of about 320000 and a sedimentation coefficient of about 12 S. The amino acid composition, subunit distribution, near- and far-UV CD spectra and immunochemical properties were identic...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90173-2
更新日期：1983-10-01 00:00:00
abstract：:Albino mice exposed to 1 X 10(-4) W cm-2 of UV radiation in the range 290-400 nm for 12 hr per day developed anterior lens opacities within 1-2 months after onset of the exposure. Histological changes demonstrated marked derangement of the lens epithelium. When the radiation was filtered with glass, eliminating radiat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80011-7
更新日期：1985-10-01 00:00:00
abstract：:The passage of glucose within the cornea has been thought to occur by passive diffusion processes. However, corneal glucose concentration profiles have not been established to support this notion. While microfluorometric methods of metabolite assay typically have been used as a means of assessing regional brain metabo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90037-1
更新日期：1988-11-01 00:00:00
abstract：:Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90150-s
更新日期：1990-10-01 00:00:00
