Abstract:
:The purpose of our study was to establish a system for culturing normal human conjunctival epithelial (NHCE) cells under serum-free culture conditions without compromising their ability to differentiate into a mucous epithelium. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing cataract surgery. Obtained NHCE cells were cultured in bronchial epithelial growth medium (BEGM) under serum free culture conditions and passage 3 cells were air-lifted. Cultured NHCE cells displayed typical epithelial morphology. Expression of cytokeratin 19 and conjunctival epithelial specific carbohydrate residue were detected. Air-lifted NHCE cells demonstrated an increase in stratification and differentiation into goblet cells up to 3weeks under air-liquid interface (ALI) culture condition. NHCE cells expressed MUC1, MUC4, MUC16, and MUC5AC mRNA, and MUC5AC production and secretion increased in a time dependent manner after culture under ALI conditions. Exposure of cells to proinflammatory cytokines (TNF-alpha or IFN-gamma) resulted in upregulation of MUC1, MUC4, MUC16, and MUC5AC gene expression. In conclusion, differentiated NHCE cells showed features of a multi-layered conjunctival epithelium, including goblet cells, and retained functional characteristics similar to those seen in vivo. This cell culture system can better facilitate investigation of conjunctival epithelial cell biology and goblet cell differentiation.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Chung SH,Lee JH,Yoon JH,Lee HK,Seo KYdoi
10.1016/j.exer.2007.04.005subject
Has Abstractpub_date
2007-08-01 00:00:00pages
226-33issue
2eissn
0014-4835issn
1096-0007pii
S0014-4835(07)00111-Xjournal_volume
85pub_type
杂志文章abstract::The advance of optical coherence tomography (OCT) enables a detailed examination of the human retina in-vivo for clinical routine and experimental eye research. Only few investigations to date captured human foveal morphology in a large subject group on the basis of a detailed analysis employing mathematical models. H...
journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107784
更新日期:2019-11-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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journal_title:Experimental eye research
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更新日期:2018-04-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1006/exer.1993.1119
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更新日期:1983-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.11.006
更新日期:2018-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1083
更新日期:1994-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.12.010
更新日期:2004-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.11.015
更新日期:2019-02-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2014.05.010
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0260
更新日期:1997-04-01 00:00:00
abstract::Argon laser energy was applied to the trabecular meshwork of pigmented rabbits in an attempt to develop an animal model of 'glaucoma'. Laser energy was varied to determine the optimal level needed to produce sustained ocular hypertension. An initial response of ocular hypertension followed by hypotension was observed ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90067-9
更新日期:1986-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2018-12-01 00:00:00
abstract::Beige adipocytes and brown adipocytes can generate heat by using mitochondrial uncoupling protein 1 (Ucp1), a thermogenic protein. Browning/beiging is the emergence of beige adipocytes in white adipose tissues (WAT) for cold acclimatization. Here we show the existence of brown/beige adipocytes in retro-orbital WAT in ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.03.021
更新日期:2019-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.10.006
更新日期:2005-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0079
更新日期:1996-06-01 00:00:00