Abstract：
:Argon laser energy was applied to the trabecular meshwork of pigmented rabbits in an attempt to develop an animal model of 'glaucoma'. Laser energy was varied to determine the optimal level needed to produce sustained ocular hypertension. An initial response of ocular hypertension followed by hypotension was observed in all of the animals tested. Approximately half of the laser-treated rabbits developed a secondary buphthalmus and sustained ocular hypertension. In these animals outflow facility was decreased by approximately 60%. Histologic examination at 4- and 8 weeks after laser treatment demonstrated a wound-healing response resulting in closure of the intertrabecular spaces and obstruction of outflow to injected carbon particles. Optic nerve cupping and a loss of ganglion cells were also observed. Topical application of L-timolol (0.5%), pilocarpine (2.0%) and forskolin (1.0%) were found to be effective in decreasing intraocular pressure in the laser-treated, hypertensive eye with no significant effect in control non-laser-treated eyes, suggesting that this model can be a useful tool for screening potential antiglaucoma medications.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Gherezghiher T,March WF,Nordquist RE,Koss MCdoi
10.1016/0014-4835(86)90067-9subject
Has Abstractpub_date
1986-12-01 00:00:00pages
885-94issue
6eissn
0014-4835issn
1096-0007pii
0014-4835(86)90067-9journal_volume
43pub_type
杂志文章abstract：:The carbohydrate epitope Gal alpha 1-3Gal-R (alpha-galactosyl epitope), which is detectable by its binding with Bandeiraea simplicifolia-IB4 lectin, was found in glycosphingolipids (GSLs), both neutral and acidic (gangliosides), from lens tissues of non-primate mammals, but not in those of human senile cataracts and O...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1151
更新日期：1994-12-01 00:00:00
abstract：:Sulphur-containing compounds in the lens were studied in relation to galactose cataract formation. Female Wistar rats were fed a 35% galactose diet and the changes in lens sulphur concentration and its distribution on a gel filtration column were compared with age-related changes. Concentration of sulphur in the whole...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90068-4
更新日期：1992-01-01 00:00:00
abstract：:Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants f...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.06.006
更新日期：2006-11-01 00:00:00
abstract：:We examine the immunoreactivity of the non-receptor tyrosine kinase, c-Src kinase and its downstream molecule, signal transducer and activator of transcription 3 (pStat3) in retinoblastoma (RB), and correlation with invasiveness and differentiation. Tumor samples from 40 patients with RB were available for the study. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.011
更新日期：2006-10-01 00:00:00
abstract：:Calf lens alpha-crystallin was isolated and the lysine residues were extensively modified with a variety of chemical agents. The effect of these modifications on elastase inhibitor activity, apparent molecular size, antibody reactivity and solubility were determined. The addition of either a methyl group or a threose ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1015
更新日期：1993-01-01 00:00:00
abstract：:Mitochondrial components are of great importance for the maintenance of lens transparency. In our previous work, we confirmed that carbon monoxide (CO) can protect human and rabbit lens epithelial cells (LECs) from hydrogen peroxide (H2O2)-mediated apoptosis, while the mechanism remains undefined. Because CO can bind ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.01.023
更新日期：2018-04-01 00:00:00
abstract：:Photoreceptor degeneration in human photoreceptor dystrophies and in the relevant animal models has been thought to be executed by one common mechanism -- caspase-mediated apoptosis. However, recent experiments have challenged this concept. In previous experiments, analyzing gene expression in the degenerating rd/rd m...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.01.014
更新日期：2006-08-01 00:00:00
abstract：:Changes in the distribution of keratan sulfate in the anterior segment of developing ocular tissue were investigated histochemically and immunohistochemically using human eyes from 5 weeks gestation to 2 years after birth. Enzyme digestion methods with streptomyces hyaluronidase, chondroitinase and keratanase showed t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1018
更新日期：1994-03-01 00:00:00
abstract：:The aim of the study was to determine anatomical and growth factor profiles in patients with clinically significant macular oedema (CSMO) undergoing pars plana vitrectomy (PPV). Twenty patients with moderate nonproliferative diabetic retinopathy (NPDR) with persistent CSMO underwent PPV. Patients had baseline and post...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.10.002
更新日期：2006-05-01 00:00:00
abstract：:Protein myristoylation occurs when the 14 carbon fatty acid, myristic acid, is covalently attached by amide linkage to a protein's N -terminal glycine by an N -terminal myristoyltransferase (NMT). A variation of this called heterogeneous acylation occurs in vivo only in retina when specific proteins are modified by my...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.1189
更新日期：2002-07-01 00:00:00
abstract：:To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0429
更新日期：1998-03-01 00:00:00
abstract：:Autophagy is a lysosomal degradative process that is essential for cellular homeostasis and metabolic stress adaptation. Defective autophagy is involved in the pathogenesis of many diseases including granular corneal dystrophy type 2 (GCD2). GCD2 is an autosomal dominant disorder caused by substitution of histidine fo...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.09.008
更新日期：2016-03-01 00:00:00
abstract：:Maintenance of cellular calcium levels is critical to cell function. Loss of calcium homeostasis might be a contributing factor to the development of cataract in the lens. In lens epithelium, calcium is involved in cell signaling and its precise regulation is vital. In this study, we investigated the regulation of sar...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.2049
更新日期：2002-11-01 00:00:00
abstract：:Raman spectra were obtained from fresh, fixed and sliced rabbit lenses and from human lens slices. For all lenses and lens slices the ratio R, defined as the Raman intensity at 3390 cm-1 divided by the Raman intensity at 2935 cm-1, was measured at different locations along the visual and equatorial axis. The ratios R ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90032-8
更新日期：1989-04-01 00:00:00
abstract：:This study tests the hypothesis that choroidal myogenic autoregulation participates in the intraocular pressure (IOP) response to mean arterial pressure (MAP) by minimizing arterial-pressure-dependent changes in choroidal blood volume. To test this hypothesis, the IOP response to MAP was quantified before and after im...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1047
更新日期：1994-05-01 00:00:00
abstract：:The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0952
更新日期：2001-03-01 00:00:00
abstract：:Recent studies of the distribution and relative concentration of caveolin-1 in fractions of bovine lens epithelial and fiber cells have led to the novel concept that caveolin-1 may largely exist as a peripheral membrane protein in some cells. Caveolin-1 is typically viewed as a scaffolding protein for caveolae in plas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.05.011
更新日期：2007-10-01 00:00:00
abstract：:To determine the effect of roscovitine, a potent antiviral in tissue culture administered intramuscularly to rabbits or by eye drops to mice for the treatment of herpetic keratitis this study was commenced.New Zealand white rabbits infected with McKrae strain herpesvirus (HSV-1) were treated twice a day with 10mg Rosc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00056-3
更新日期：2003-06-01 00:00:00
abstract：:For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.03.027
更新日期：2018-07-01 00:00:00
abstract：:Anti-Vascular Endothelial Growth Factors (Anti-VEGF) agents have received recent interest as potential anti-fibrotic agents for their concurrent use with trabeculectomy. Preliminary cohort studies have revealed improved bleb morphology following trabeculectomy augmented with ranibizumab. The effects of this humanized ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.08.005
更新日期：2014-10-01 00:00:00
abstract：:Lens epithelium-derived growth factor (LEDGF) has been shown to enhance survival of lens epithelial cells (LECs) against stress. The objectives of these studies are to determine how LEDGF controls PKC gamma activity in normal LECs: how this control of PKC gamma regulates the phosphorylation of Connexin 43, the inhibit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00049-6
更新日期：2003-05-01 00:00:00
abstract：:Corneal epithelial defects are a common cause of ocular morbidity and can result in corneal scarring if they do not heal properly. Matrix metalloproteinases (MMPs) are extracellular matrix proteinases that regulate multiple aspects of corneal repair. We have previously shown that MMP12 has a protective effect on corne...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.04.007
更新日期：2017-07-01 00:00:00
abstract：:The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107869
更新日期：2020-01-01 00:00:00
abstract：:Optic fissure closure defects result in uveal coloboma, a potentially blinding condition affecting between 0.5 and 2.6 per 10,000 births that may cause up to 10% of childhood blindness. Uveal coloboma is on a phenotypic continuum with microphthalmia (small eye) and anophthalmia (primordial/no ocular tissue), the so-ca...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.107940
更新日期：2020-04-01 00:00:00
abstract：:A simple culture method that was recently developed in our laboratory was applied to the chick iris tissues to characterize neural stem/progenitor-like cells. Iris tissue is a non-neuronal tissue and does not contain any neuronal cells. In the present study we isolated iris tissues from chick embryos just prior to hat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.04.007
更新日期：2014-06-01 00:00:00
abstract：:The suitability of the rat derived SV-40T immortalized RPE-J cell line for identifying proteome changes associated with RPE differentiation was evaluated by surveying changes in protein expression levels. Rat RPE-J cells were induced to undergo differentiation in culture by growth at the nonpermissive temperature of 4...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1058
更新日期：2001-10-01 00:00:00
abstract：BACKGROUND:The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. Thi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108392
更新日期：2020-12-15 00:00:00
abstract：:Keratoconus (KC) is a progressive, early onset, and often bilateral eye condition, in which the cornea gradually weakens and bulges out, and in advanced cases may eventually become cone-shaped. The available evidence suggests that it is a multifactorial disease with environmental and genetic contributions. Matrix Meta...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.03.016
更新日期：2019-05-01 00:00:00
abstract：:The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00068-x
更新日期：2003-07-01 00:00:00
abstract：:The biomechanical environment within the optic nerve head (ONH) is complex and is likely directly involved in the loss of retinal ganglion cells (RGCs) in glaucoma. Unfortunately, our understanding of this process is poor. Here we describe factors that influence ONH biomechanics, including ONH connective tissue microa...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.02.005
更新日期：2017-04-01 00:00:00
